[in Japanese]

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It was made clear that the thoracic muscle of insects (especially honey bees and dragonflies) had peculiar metabolic pattern, which was different from the other skeletal muscle. The conclusion was as follows :
1. Oxygen consumption of thoracic muscle homogenate of dragonfly was largest when a-glycerophosphate was used as substrate.
2. α-Glycerophosphate oxidase activity of the dragonfly and the honey bee thoracic muscle was much stronger than the sparrow breast and femoral muscle, the rat liver and femoral muscle, and the toad femoral muscle.
3. In a homogenate of rat femoral muscle added α-glycerophosphate scarcely decreased, and some lactic acid accumulated aerobically. However, in a homogenate of bee thoracic muscle added α-glycerophosphate decreased remarkably and lactic acid scarcely accumulated.
4. α-Glycerophosphate oxidase of the dragonfly thoracic muscle was localized in the mitochondrial part of the cells.
5. α-Glycerophosphate oxidase activity of the dragonfly thoracic muscle was strengthened in the isotonic KCl medium by Mg⁺⁺, Ca⁺⁺ and HPO₄^--. In the isotonic NaCl medium, it was strengthened by Ca⁺⁺ and HPO₄^--, but rather inhibited by Mg++.
6. α-Glycerophosphate oxidase activity of the dragonfly thoracic muscle was inhibited by EDTA but it was recoveved Stored by Mg⁺⁺.
7. α-Glycerophosphate oxidase activity of the dragonfly thoracic muscle was inhibited by IAA and PCMB, but it was not affected by DNP, arsenite, and malonate.
8. The absorption spectrnm of reduced cytochrome c, which was isolated from the dragonfly thoracic muscle and the sparrow breast muscle, was coincident with that of the beef heart muscle. Cytochrome c content of the dragonfly thoracic muscle was 79.2 mg, and that of the sparrow breast muscle was 41.6 mg per 100g fresh tissue.
9. The dragonfly thoracic muscle produced anaerobically, much smaller amount of lactic acid comparing with the consumption of endogenous carbohydrate.
10. In anaerobic condition the honey bee thoracic muscle produced α-glycerophosphate and pyruvic acid, but lactic acid was only in a small amoumt. In a homogenate of honeybee thoracic muscle 1 mole α-glycerophosphate and 1 mole pyruvic acid were produced. from 1 mole of added fructose-1, 6-diphosphate, the production of lactic acid being scarcely observed.
11. The relation between the function and metabolism of the inset thoracic muscle was discussed.

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1. In fractionating α-glycerophoshate in the extract from the animal tissue, Sacks's method is complicated and needs much time. The author simplified Sacks's method by omitting the fractionation of adenylic acid and glucose-6-phosphate on the basis that there were much smaller quantities of them than α-glycerophosphate in the honey bee and the dragonfly thoracic muscle. So, the author ascertained that it is possible to determineα-glycerophosphate by the author's method.
2. The recovery test of α-glycerophosphate by the modified method showed that about 95% of added α-glycerophosphate could be recovered.
3. The quantity of α-glycerophosphate in honey bee and dragonfly thoracic muscle was 159mg, and 111mg per 100g fresh tissue respectively.

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An autopsy case of disseminated aspergillosis which continued from the initial lesion of tuberculous lymphadenitis in a 44-year old female farmer was discribed.
In this case, the fungus lesion was most remarkable in lungs and the lesion of the blood vessels caused by aspergillus was most severe as compared with common localized pulmonary aspergillosis.
Therefore, it is supposed that dissemination of the fungus in other several organs. was hematogenous from the focus in The lungs.
On the other hand, it is concidered that administration of both excessive amount of steroidhormone and antibiotics encouraged its infcetion and dissemination, in addition to operation stress of ilues in a few clays before death.

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The virulent strain of the S. entelitidis was infected intracutaneously to the mice and the course of infection was studied.
1. The least dose, 10^-8mg of the microorganisms was enough to kill all the mice infected, and the survival time shortened gradually with the increase of infectious dose.
2. When 10^-7mg of the bacteria was infected intracutaneously, they promptly multiplied 100 fold within 24 hours at the infected locus. In the skin, the bacterial growth reached almost the maximum 2 to 3 days after the infection. At the same time the infected bacteria in the local lymph node began to increase and they reached maximum cell density about 5 days after infection. Then the bacteria entered into the blood stream and were taken up by the reticulo-endothellial system. In the systemic infection thus formed, the cell number in the liver increased linearly until they reached about 10⁸/g, when the mice died.
3. The mice died in the intracutaneous infection later than in the intravenous infection. This prolongation may be due to the localization of the infected bacteria within the skin and lymph nodes in the case of the intracutaneous infection.
So in the experimental salmonellosis when infected intracutaneously, it must be considered that the infectious course is separated into the local and the systemic stadium.

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Intraperitoneal inoculation of Leptospira icterohaemorrhagiae (Mikawashima strain) was made in 3-week-old mice of ddN strain, it produced various of Jaundice and petechial bleeding mainly in the subcutaneous layer of the skin and in the lungs.
Histopathologic studies of these mice revealed following changes cloudy swelling, slight degeneration and necrosis of the hepatic cell in various places and the dissociation of the hepatic cell cord, necrosis characterized by degeneration and necrosis of tubular epithelial cells, vacuolation and hyaline degeneration at certain points of individual skeletal muscle fibers, and intraalveolar haemorrhage in the lung.
Leptospirae was demonstrated by Levaditi's or Dietrele's silver impregnation method in the liver, kidenys and skeletal muscle fibers.
The changes in the liver, kidneys and in the skeletal muscle, and petechial haemorrhage were observed to have developed in preicteric stage.

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This report deals with a male patient of 6 years, succumbed to testicular tumor origeniating in the left testis with widespread metastatic tumors in the whole body after extirpation.
1. Gross findings
1) Operative specimem : Hen's egg sized, soft, covered with thick white capsules and divided into many nodules by a small amoumt of connective tissues.
2) Metastatic tumor formations around the abdominal aorta and pelvic space having sizes of an infant's head and a fist, respectively.
3) Swelling of liver with numerous metastatic lesions.
4) Numerous metastatic lesions of omentum, diaphragm and lymphonodules around the gastric wall.
5) A pea-sized metastatic tumor formation in upper lobe of right lung.
6) In addition, cerebral swelling, hydronephrosis of left kidney and dilatation of left ureter were main findings.
2. Microscopic findings
Microscopicallg the tumor consisted of two types of cells. One had wide acidophilic cytoplasm with ovoid nucleus poor in chromatin and often showed large acidophilic inclusions bodies. The other had obscure cytoplasm with ovoid, chromatinrich nucleus and distinct nucleolus. Polymorphisms were dominant in a latter cell group. The former showed less polymorphisms and inclined to form cell masses or giant cell formation.
The tumor was rich in blood vessels and in some part showed radial arrangement around the vessel wall. But as a rule the tumor tissue had less resemblance to the mature tissues.
It is considered that this tumor belongs to a type of embryonal carcinoma on account of the above mentioned histological pictures.

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