In experimental Salmonellosis, produced in mice with S. enteritidis, Mice, immunized with 10^-5mg of an attenuated strain of S. enteritidis (SER), and 3 weeks threeafter reimmunized with 10^-7mg of a virulent strain of this bacillus, achieved high grade immunity and survived the challenge of 1, 000MLD of the virulent strain.
On the other hand immunized mice acquired hypersensitivity against the endotoxin of S.enterisidis and showed marked hyaline granules in the liver cells accompanied by severe damage.
In the previous papers, we reported the experimental condition of the appearance of hyaline granules and histochemical characters.
In this report, we performed the nonspecific immunization or repeated immunization with endotoxin and both results in hypersensitivity against the endotoxin and appearance of hyaline granules.
Mice immunized with living vaccine of S. enteritidis showed anaphylactic shock against the endotoxin of E. coli which had no common antigens with S. enteritidis and died with in 24 hours accompanied by marked hyaline granules.
In the other group, imumnization was done by repeated subcutaneous and intraperitoneal injection of the endotoxin of S. enteritidis amounting to 0.15mg in total with in 25 days. Anaphylactic shock was induced by the same endotoxin injection given after the interval of 10 days and appearance of hyaine granules was conspicuous.
It is considered that hypersensitivity against the endotoxin nonspecific with regard to the O-antigen and immunization with the living vaccine of S. enteritidis induced antilethal resistance against the infection with the virulent strain of living vaccine but repeated immunization with endotoxins in the subcutis and the peritoneal cavity, produced hypersensitivity against the same endotoxin accompanied by marked hyaline granules.
It seemed interesting to us that interrelation was found between the hypersensitivity and appearance of hyaline granules.

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Experimental studies on small vessel anastomosis, small arterial transplantation and closure of small vessel incision were done using 102 mongrel dogs by hand suture method.
Significances of operative binocular microscope, suture material (Silk and Nylon monofilament and plastic adhesive (Aron-Alpha 201, 202 and Eastman 910) were also investigated in small vessel surgery.
1) In end to end anastomosis of small arteries, patency rate was 90% in nylon thread suturing with plastic adhesive, It is considered that the suturing procedure must be done under operative binocular microscope in the arteries less than 2.9mm of external diameter, and that the arteries more than 3.0mm can be sutured under the naked eye observations.
2) In end to end anastomosis of small veins, patency rate was obtained 90% in every method because of their large size of diameter.
But hand suture anastomosis of mesenteric vein was so diffcult that it should be anastomosed with mechanical apparatus.
3) The most excellent substitute for small arteries was autogenous veins, and the patency rate was 85% in this grafting.
4) patency rate in simple closnre of incised vessels using plastic adhesive was 100% with and without suturing.
5) In operative procedures of small vessels, all layer of vascular wall were sutured roughly and then sutured area was glued over with plastic adhesive.
By doing so, stenosis and bleeding in the site of anastomosis could be almost prevented. In practice, this method is significant to supplement the imperfection of mechanical suturing apparatus and microscopic method.
6) Hand suture method using plastic adhesive was applied in 45 clinical cases, and it obtained very effective results.

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In order to study the histological and biological features of experimental gliomas, transplantation experiments were made with the following results.
1) By implanting methylcholanthrene pellets into the brain of various strains of mice and rats, a total of 48 gliomas were produced. They were transplanted into the subcutis of the back of the same strain of animals. Twenty-eight transplanted tumors yielded positive “takes” and of these, 16 gliomas were capable of subsequent transfers through more than 4 generations.
2) Microscopically, the transplants of early generations recapitulated in some measure the features of the histology of the parent tumors. After subsequent transfers, they became more simplified in cell composition and more undifferentiated in cellular appearance, irrespective of their histological types of the primary neoplasms. According to the cytological features of the constituents cells the transplanted tumors over several generations were classified into three types.
3) The transplanted gliomas showed occasionally variation of the histology into mesenchymal tissue tumors. In six cases, the tumors appeared as sarcoma, after they had been transplanted repeatedly. It is argued that this suggests the frequency of the primary tumors which were composed of different component parts, glioma and sarcoma.
4) Experimental gliomas were transplantable into the various parts of the body of the homologous strains and species of animals : the brain, subcutis of the back and tail, anterior chamber of the eye, abdominal cavity, and the kidney. Injections of a suspension of the tumor cells into the vein of the tail also developed multiple tumor growth in various of the body. No significant variations of the histology were visible according to the sites of the tumor growth. The transplantation rate was considerably higher when trnasplanting yonger animals than employing adult ones.
5) Transplantation of experimental gliomas into heterologous strains of animals were also possible. The transplanted tissues showed local growth in the brain, anterior chamber and the subcutis of the back. No variations in the histology were noted between the original and heterologous transplants. In several cases, however, evidences of cell differentiation were observed in the histology of heterologous transplants.
6) Transplantation experiments were also made on the allantoic membrane of the chick eggs. The transplanted cells showed an evidence of growth with highly anaplastic cellular appearance.

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In order to clarify the pathogenesis of hypothalamic ovarian insufficiency, a series of basic and clinical experiments were carried out.
Continuous administration of β- phenyl-isopropyl-hydrazine (monoamine oxidase inhibitor MAOI) to immature female rats induced the retarded vaginal opening and initial estrous, and the reduction of the pituitary, ovarian and uterine weight and of the pituitary gonadotropic potency. Single administration of the same MAOI to mature female rats increased the pituitary gonadotropic potency significantly. These results suggest that the metabolism of the amines in the hypothalamus may play some role in regulation of the gongdotropin secretion from the pituitary.
Electrolytic lesions of the hypothalamus, used with the Krieg's stereotaxic instrument, induced the two types of the experimental ovarian dysfunction in rat, that is the constaut estrous rat and thd constant diestrous rat. Gonadotropic potency in rat pituitary inereased in the former and decreased in the latter.
The hypothamic function in 65 cases of the amenorrheic women was tested, using with Mecholyl test, Horten' s water test, X-ray test of the sella turcica and electroencephlographic test. 41 women with hypothalamic amenorrhea showed abnormal results in Mecholyl test. The results of the Horten's water test was not parallel with the grade of amenorrhea and the results of the Mecholyl test. 4 cases among 32 amenorrheic patients showed the enlarged sella turcica and 8 cases showed the deformed sella turcica.
In the electroencephalography after activation by Pentamethylentetrazol injection in 39 cases of the amenorrheic women, θ-burst in 6 cases and spike and wa e in 12 cases were observed respectively. The frequency of such abnormal EEG wave was significantly higher in the normal women, so this test has diagnostic value in evaluation of the amenorrheic patients.
The influeuce of surgical stress to the woman ovulation and menstruation was different between in the follicular phase and the luteal phase.
The urinary hormone analylis in the amenorrheic aud psychiatric women, inducing 20 cases of schizophrenia, 5 cases of the other psychiatric diseases and 3 cases of epilepsy, demonstrated that the amenorrhea combined with psychiatric disorders belongs to the so-called “hypothalamic amenorrhea”.

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