(1) Using the pollen of Pinus Thumbergii as an antigen, allergic conjunctivitis was experimentally provoked in male albino rabbits, and by means of fluorescence, relation between the circulating antibody 2nd the tissue antibody on the 35th day after the beginning of the experiment, and the effect of 6 -MP on the antibody were observed.
(2) Fluorescences in the whole area of the conjunctiva were conspicuously more numerous in the sensitized than in the non-sensitized group, and the circulating antibody and antibody-producing (or containing) cells were approximately in parallel relation.
(3) Comparison between the antigen-administered and the non-administered eye samples revealed that fluorescences were significantly more numerous in epithelial cells of the foimer than in those of the latter.
(4) When the production of the circulating antibody was inhibited by oral administration of 6-MP, a mitotic poison, the incidence rate of fluorescence was lowered. And there was ample parallel relation between the fluorescence and the circulating antibody.
(5) One and the same sample was twice stained with hematoxylin-eosin to identify the antibody producing or containing cells, and it was confirmed that not only lymphocytes and plasma cells but also eosinophils and pseudo-eosinophils were fluorescent.
(6) As reported in the previous paper, eosinophils and pseudoeosinophils were observed more remarkably in the sensitized than in the non-sensitized animals. Specific features of their fluorescence will be studied later.
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