Using the bridge-voltage clamp method after Matumoto et al., current flow during and after application of the depolarizing clamped voltage on the skeletal muscle fibers separated from the bullfrog was observed. The obtained results were the following.
1. During application of the depolarizing clamped voltage, the current change due to increase of the apparent resistance by the polarized potential caused by the applied voltage (polarization current) occurred and with increasing the clamped voltage a transient inward current (peak current) associated with the polarization current occurred.
2. The peak current occurred early after application of the clamped voltage when the clamped voltage was large, and occurred occasionally several times rhythmically.
3. After the clamped voltage turned to zero, the polarization current reduced exponentially, but the peak current reduced with a course similar to that when the clamped voltage continued longer.
4. During the peak current the resistance of the muscle fibers reduced, and this had no special reference to Na-ion.
5. The polarization current was assumed to be produced by the polarization of the tissue constituting the muscle fiber and the peak current by the action potential of the muscle fiber.

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Antibody reacted with urine from cancer patients was produced in the rabbit sera immunized with the urine from gastric cancer patient which inhibited the reaction of anti-gastric cancer immune serum. Specimens of the intact urine from cancer patients showed the direct precipitation reaction with the anti-cancer urine serum by the agar gel diffusion technique, and the reaction of anti-cancer urine serum was inhibited by most of concentrated urine from cancer patients. The reaction was inhibited by D-glucosamine and D-galactosamine as simple sugars just like the reaction of anti-gastric cancer immune serum.

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Organ distribution of ⁶⁵Zn injected i.p. or s.c. into Ehrlich ascites cancer-bearing mice was examined with the following results.
1) Distribution of ⁶⁵Zn injected i.p. was examined as a function of time (1-24 hr). ⁶⁵Zn was found mainly in ascites cancer cells reaching a maximum (26%) 6 hr after the injection and its concentration was gradually decreased thereafter. Radioactivity of ⁶⁵Zn in liver, kidney, pancreas and spleen were less than that in cancer cells. Disappearance of the radioactivity contained in cancer cells was very little after washing.
2) Subcellular distribution pattern of ⁶⁵Zn in ascites cancer cells was examined extending from 1 to 24 hr after i.p. injection. The amount of ⁶⁵Zn in supernatant fraction was highest followed by nuclear, microsomal and mitochondrial fractions in the descending order and the above distribution pattern was same at any times.
3) Radioactivity of ⁶⁵Zn administered s.c. into cancer-bearing mice was significantly increased in the liver and decreased in pancreas and blood with no changes in the kidney and spleen compared with that in normal mice. After the i.p. injection of ⁶⁵Zn into cancer-bearing mice, 66% of total radioactivity was found in ascites and ⁶⁵Zn concentration in the liver, kidney, pancreas, spleen and blood were less than that in normal mice.
4) When the radioactivity of ⁶⁵Zn injected i.p. or s.c. in the subcellular fractions of liver and cancer cells are expressed per mg protein, the highest concentration of ⁶⁵Zn in the liver was found in the supernatant fraction followed by microsomal, nuclear and mitochondrial fractions in the decending order. These distribution pattern of ⁶⁵Zn was equal with that of cancer cells.
5) ⁶⁵Zn in the liver and cancer cells binds loosely with protein. After the treatment of each subcellular fractions obtained from liver and cancer cells with (NH4) ₂SO₄ (70% saturation) and trichloroacetic acid (10% in final), most of ⁶⁵Zn was distributed in supernatant excepting the liver nuclear fraction in which most of radioactivity exists in precipitate by (NH₄) ₂SO₄.
6) Subcellular distribution of ⁶⁵Zn in mammary carcinoma was examined 24 hr after the s. c. injection. ⁶⁵Zn concentration is equal in nuclear and supernatnat fractions followed by microsomal and mitochondrial fractions in the descending order. The distribution pattern of ⁶⁵Zn in nuclear fraction was different from that in the liver.

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Various drugs (anti-cancer agents, chelating agents, amino acids and salts) were injected i.p. with ⁶⁵Zn into Ehrlich ascites cancer-bearing mice, and after 1 hr its effect on the organ distribution of ⁶⁵Zn was investigated.
1. Mitomycin C (200 μgg/mouse), Toyomycin (50 μg/mouse) and Nitromin (500 μg/mouse) had no effects on the distribution of ⁶⁵Zn. Merphyrin (500 μg/mouse) caused a decrease in ⁶⁵Zn uptake by cancer cells without any effects in the other organs.
2. EDTA and BAL (each 1 mg/mouse) inhibited ⁶⁵Zn> uptake by cancer cells and conversely elevated its concentration in ascites supernatant. BAL elevated also ⁶⁵Zn concentration in other various organs. Tetracycline, dihydrothioctic acid and Na-thioglycolic acid (each 1 mg/mouse) did not affect the ⁶⁵Zn uptake by cancer cells.
3. Penicillamine (D-and DL-type, each 1 mg/mouse) caused a decrease of ⁶⁵Zn concentration in cancer cells with an increase of its concentration in the other organs. Cysteine, histidine and glycine (each 1 mg/mouse) had no effects on the ⁶⁵Zn distribution in every organs.
4. Various salts (each 0.1 ml of 3.5 ×10^-2M solution was injected per mouse) also affected organ distribution of ⁶⁵Zn. ZnSO₄, CoCl₂ and NiSO₄ inhibited the uptake of ⁶⁵Zn by cancer cells, however, MgCl₂, MnSO₄, FeCl<₂ and CuSO₄ had no effects on it. Organ distribution of ⁶⁵Zn was also affected by various salts without definite relationship between them.
5. In ascites cancer cells, the highest concentration of ⁶⁵Zn was found in supernatant fraction, next in nuclear and microsomal fraction. The mitochondrial fraction contained the lowest amount. All of the above drugs had no effect on the intracellular distribution pattern of ⁶⁵Zn in cancer cells.

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With a test method devised by Matumoto et al., which was an application of putting in the golf play, the grade of skill for the action requiring fine control, which an individual subject attained after the training of the primary step, was intended to measure. The training of the secondary step was carried out after 3 months on the subjects (5 females and 5 males) who performed the training of the primary step (one time of training course on every day over 30 days). The results were the following.
1. The score at the beginning of the test in the secondary training was higher than that in the beginning of the primary training on all subjects.
2. The curve of learning effect for the individual subject ascended more steeply than the one in the primary training on all subjects and soon reached to the maximum level which was attained by the subject in the training of the primary step.
3. The curve of learning effect reached to a plateau after the training of 40-50 days and this level was maintained when the training was continued there after.
4. The height of the plateau of the curve of learning effect, namely the grade of the skill was different for each subject.
5. The grade of the skill had little relation to the curve of learning effect in the training of the primary step.
6. At the plateau state of the curve of learning effect, the score shaked daily, the shaking was little on some subject but was large on the other.

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Fluorescence changes following the excitation in a Pacinian corpuscle were examined under the fluorescence-microscope by using ANS (Anillino naphthalene-sulfanic acid) or Acridine-orange which were injected through the cat's saphenous vein. And the observation of the fluorescence on an Ameba membrane was done too.
1) An increase of fluorescence was taken as a picture after repetitive stimuli when a certain concentration (0.5 to 1.0% 1ml/Kg) of these dyes was used.
2) When the stimuli were not applied to the corpuscle any increase was not shown on the picture.
3) If the fluorescent dyes were directly added in Ringer-Locke's solution not in the vein, any fluorescence was not seen in the corpuscle even after repetitive stimuli.
4) The excitable membrane was not permeable to fluorescent dyes because any Pacinian corpuscle couldn't elicite the fluorescence by means of dyes which were directly applied in the outer solution.
5) Ameba proteus also couldn't elicite the fluorescence at 0.002% to 0.1% concentration of ANS when the cell membrane was sustained normality. As soon as the membrane was broken the protoplasma fluorescenced a light green color.

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A mass survey for stomach cancer was performed in Gunma Prefecture since ten years ago. The gastric mass survey has been called the Gunma-Method and 258, 090 cases have been examined.
As the primary screening test, Shichijo's reaction (a non-specific, biological test for gastric cancer) was adopted, combining with indirect radiography and stomach complains of the patients.
From this survey, 620 cases of gastric cancer including 134 cases of early gastric cancer were detected. The detection rate of the former was 0.24% and that of the latter was 0.052%. About 25 per cent of the cases received the primary screening survey were needed the more detailed examination, and this percentage was a little higher than that of other screening methods reported by others in Japan.
In an early period of our survey, the primary screening test was consisted of the patient's complains and Shichijo's test without radiography. The recent screening test including the indirect radiography, however, made a significant improvement of the detection rate, namely, the rate of gastric cancer was 0.29% and that of early gastric cancer was 0.065% in the recent survey.
Shichijo's reaction had been developed to detect gastric cancer and had given notice that in gastric cancer the positivity rate was much high (8090%). But in the early gastric cancer detected in mass survey, the positive reaction accounted for 28 of the total 118 cases (23.7%), that was significantly lower than those in out-patient clinic.
Therefore, this reaction alone was not satisfactory for the screening test. It should be adopted, however, as a supplementary examination, since gastric cancer in the positive cases of this reaction was detected ninetimes as many as in the negative cases. In the types of gastric cancer in cases of mass survey, type II c (depressed type of early gastric cancer) was observed mostly (60%). Type I (protruded type), type IIa (elevated type), type IIa-IIc and type III (excavated type) were observed a little and their each percentage were only 10%. In advanced gastric cancer, Borrmann III type was observed mostly (60%) and Borrmann II type 20%. Borrmann I and IV type were only 10% each other. In ages, the cases of gastric cancer in the mass survey were detected mostly in the fifth decade, and interestingly, in much older age of the sixth decade in out-patient clinic. The five-year survival rate of gastric cancer in the mass survey showed better prognosis than in out-patient clinic, that is, in mass survey the five-year survival rate of early gastric cancer was 97% and of advanced gastric cancer was 45.6%; in out-patient clinic the former was 91% and the latter 16%.
Since 1968, the statistical death rate by gastric cancer has been lowered in Gunma Prefecture. The decline of this death rate might be due to the results of this mass survey.

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