PEER, FEV_1.0 and FVC of children with bronchial asthma in comparison with those of healthy children of similar size-% PEFR, % FEV_1.0 and %FVC-were used as the indicators for the judgement of the therapeutic effect in childhood asthma. Those indicators were proved to correspond well to the change of severity of the asthmatic patients when supplied as the indicators for the effects of the inhalation therapy. Among those indicators, only %PEFR could indicate the effective limit of the inhalation therpapy. Namely, it became clear that when the %PEFR was under 20%, the attack could not be cured by inhalation therapy alone. From the above result, it can be concluded that the %PEFR using the peak flow meter is most useful as the clinical indicater. This apparatus has addition al benefits, as it can be usedby anybo dy, anywhere, every time and with ease.

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PEFR of asthmatic children was measured by Wright's peak flow meter every morning. By comparing PEFR of asthmatic children with non-asthmatic children of the same age, % PEFR was obtained.
The relationship between % PEFR in the morning and the incidence of the cases which required inhalation therapy was investigated during the following three periods, namely 1) during school hour, 2) daytime (morning till night) and 3) full day (morning till the following morning).
As a result of this, it is noted that the incidence of the cases which necessitate inhalation therapy to be higher in the low %PEFR group than the high %PEFR group, and this statistical observation may offer valuable suggestions to the daily life management for asthmatic children at the protective institution.

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By in vitro and in vivo cellular immunologic test using a panel of antigens as SK-SD, PPD, and PHA, the delayed hypersensitivity in 6 patients with JRA and a patient with RA was found to be considerably impaired.
In an attempt to restore the immunosuppression in the JRA and RA, transfer facter was clinically applied to those patients with JRA and RA. There was a striking improvement in 6 out of 7 cases, concomitant with the development of cellular hypersensitivity to the antigens. But one patient with JRA showed neither any amelioration in general condition nor convertion of the skin reactivity. No untoward side effects of transfer facter have been apparently recognized.
These clinical results suggested that clinical application of transfer facter to JRA were more valuable to promise further study.

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In the previous papers, Matsumura et al. reported that food sensitivity play an important role on the etiology of postural proteinuria (PP). Namely, PP was obviously induced by trial ingestion of allergenic foods such as milk, egg, soybean, pork, redbean and/ or mackerel, and clearly removed by their elimination. Avoidance also made the accompanying symtoms improved. PP in this series dealt with the proteinuria induced by the erect lordotic posture. In other words, our subjects should strictly be called lordotic proteinuria (LP).
In this paper, 9 cases of LP, aged 5 to 13 yrs. and consisted of 6 girls and 3 boys, were reported, in which LP still persisted even after eliminating a few or more suspected foods and finally disappeared or markedly improved by eradicating infectious foci such as inflammed tonsils and adenoid as well as decayed teeth. The periods necessary for inducing improvement were 2/3- 12 (average 3.9) months after eradication. The infectious foci removed were as follow carious teeth in 1 case ; tonsils in 3; carious teeth and tonsils in 4 ; tonsils and adenoid in one. On light microscopy of 2 renal biopsy specimens, one showed minimal abnormalities including a focal slight increase in mesangial cells, and another a diffuse slight mesangial proliferation. In the latter, immunofluorescent study revealed deposits of IgM in mesangial regions and walls of vasa afferentia as well as along the capillary loops. Deposits of IgG and β₁C were also seen along the peripheral capillary walls of glomeruli. On the electron microscopic examination, focal foot process fusions and so on were observed in both cases.
From these findings it might be suggested that focal infection coexisting with food sensitivity play an important role in the pathogenesis of postural proteinuria.

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Phenol soluble proteinaceous substance (PSPS) was extracted from the whole cell of E. coli using the Westphal's phenol-water method.
The procedure was similar to that of the extraction of protodyne (Berger et al) excepting some difference in the starting materials of whole cell for extraction in the former and, in the latter, of the cytoplasma, previously isolated from the cell wall.
The results in regard to the chemical and biological characteristics of PSPS were as follow :
1) PSPS consisted mostly of proteins ; small amount of hexose and ribose, none of desoxyribose.
2) Using the Sephadex G-200 column chromatography the PSPS was separated into 3 fractions with the high absorption of 280 mμ.
3) Molecular weights were determined by Andrews' method as having 150, 000 for Fr I, 30, 000 for Fr II and 10, 000 for Fr III, respectively.
4) PSPS has an ability to induce the some degree of Shwartzman reaction in the rabbits, which was far less than that of endotoxin.
5) Homocytotropic antibody was produced by the PSPS incorporated in alum-gel as adjuvant; reached the maximum titer at 3 weeks; remained the high titer at 4 weeks after the immunization
In conclusion, PSPS looks like the protodyne as to the chemical characteristic except for the contamination with small amount of endotoxin, because of its relatively high contents of sugar and of inducible activity of the feeble Shwartzman reaction in rabbits in comparison with protodyne. From these results PSPS should be much more purifed by means of column chromatography to have an exclusively proteinaceous substance just like the protodyne.

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In the previous manuscript, authors reported that PSPS consisted mostly of proteins with small amounts of hexoses and riboses ; that revealed and ability to induce feeble Shwartzman reactions in rabbits and to produce the homocytotropic antibody in rats with relatively high titers. In this paper, PSPS had another aspect of biological activity as an inhibitory effect upon the TdR incorporation into human leucocytes stimulated with PPD, streptococcal M-protein and SK-SD, respectively.
The results obtained were as follows :
1) PSPS was able to stimulate the human leucocytes of healthy subjects in 2 out of 7 persons in whom stumulation index (S. I.) revealed 3 and 13, respectively.
2) On the contrary, PSPS inhibited the TdR incorporation into leucocytes stimulated with PPD, streptococcal M-protein and SK-SD when that was given simultaneously with those antigens to a large extent of showing more than 70% of percentage inhibition in an individual case.
3) Of particularly curious thing was that in a case (donor T. K.) in which the PSPS obviously stimulated the leucocytes with an increased TdR in corporation, inversely that inhibited TdR incorporation with approximate 70% of percentage inhibitions when was given simultaneously with specific antigens.
4) There was a trend toward more increment of percentage inhibition in such a condition that the PSPS was given simultaneously or 2 hrs before the antigen stimulations with PPD, streptococcal M- protein and SK-SD in comparison with that 2hrs after antigen stimulations, suggesting a possible involvement of competitive binding between the receptors for antigens and for PSPS.
5) Trypan blue exclusion test was carried out in order to exclude the cytotoxic activity of PSPS upon the leucocytes with the resultant data of over 80% viable cells remaining at 3, 4 and 5 day cultures.
Thus, though PSPS was able to stimulate those leucocytes with the increased TdR incorporations in a part of healty subjects, that inversely inhibibited the TdR incorporations when was stimulated simulutaneously with the specific antigens. The mechanisms underlying these mutable contradictory actions of PSPS upon the human leucocytes should be more clarified on the basis of its chemical purification and mutual competitive inhibition of cell surface receptors involved in the regulation of DNA synthesis in nucleus.

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