A phosphatidylinositol bisphosphate phosphatase (EC 3.1.3.36) has been purified 100-fold from the ox brain 105, 000 xg supernatant fraction by ammonium sulfate fractionation, DEAE-cellulose column chromatography, second ammonium sulfate fractionation, Phosphocellulose column chromatography and Sephadex G-200 column chromatography. Five percent polyacrylamide dise-gel electrophoresis showed that the main protein band exhibited the activity of phosphatidylinositol bisphosphate phosphatase. The enzyme was highly specific to phosphatidylinositol bisphosphate. The reaction product was identified as phospha-tidylinositol monophosphate. The Km value for phosphatidylinositol bisphosphate was calculated to be 6.67×10^-4M. Phosphatidylinositol monophosphate was a poor substrate. Cetyltrimetylammonium bromide and MgCl₂ were required for the full activity of the enzyme. No phosphatidylinositol bisphosphate phosphodiesterase activity was detected in the purified preparation.

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Yoshida sarcoma cells labeled with ¹³¹I-iododeoxyuridine were inoculated into Donryu female rats intraperitoneally. In these animals, the fractional rate of cell death and migration from the peritoneal cavity were monitored by measuring the radioactivity in the whole body and extraperitoneal site with a whole body animal counter. The radioactivity of the tumor cells in ascites fluid was measured by a well type scintillation counter. ³H-TdR-labeled Yoshida sarcoma cells were inoculated into Donryu female rats intraperitoneally. The radioactivity in the DNA fractions from the lungs, liver, spleen, kidneys, solid tumor on the omentum and the tumor cells in the ascites fluid was measured by a liquid scintillation counter 24 and 96 hours after the intraperitoneal inoculation in order to chase changes in the anatomical distribution of the ³H-labeled tumor cells. The results are as follows : (1) The death rate of ¹³¹I-IUdR-labeled tumor cells inoculated intraperitoneally was 6.3% per day. Six hours after the inoculation of the ¹³¹I-IUdR-labeled tumor cells, 18.5% of the radioactivity were in the extraperitoneal tissues, indicating rapid migration of tumor cells from the peritoneal cavity. The extraperitoneal radioactivity did not change until 72 hours after the inoculation, then it was increased at a constant rate to 32.1% 120 hours after the inoculation. The rate of cell loss from the ascites fluid was 9.2% per day until 48 hours after inoculation and thereafter it was increased to 17.0% per day. (2) Radioavtivity present in the lungs, liver, spleen and kidneys 24 hours after the inoculation with ³H-TdR-labeled tumor cells was 1.27±0.05%of the total amount of radioactivity administered. Ninety-six hours after the inoculation, radioactivity distributed in these organs and solid tumor on the omentum was 8.49±0.19%. From these results, solid tumor on the omentum, liver and lungs were seemed to be the major place of the tumor cell migration. It was concluded that the tumor cell death and the migration from the peritoneal cavity might participate a deceleration in the growth curve of the tumor cells in ascites fluid.

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1959年にCameronらは本来 A₁ (β) 型であった7例の患者血球が抗B (β) に凝集するようになった特殊な例を報告し, この弱いB型様抗原は後天的に獲得 (acquired) されたもので, 遺伝性はなく, 高年齢あるいは疾病, 特に直腸及び結腸がん又は両方に関連があるのではないかと述べている.Gilesらは遺伝子型が A₁A₂型の結腸がんの患者の血球が弱いB型様抗原を獲得した例を, MarshらもA₁型の同様な症例を pseudo Bとして報告した.その後, A型の直腸がん結腸がん, 幽門狭窄症, 胃がん, 腹膜炎, 慢性白血病, 腸閉塞, がん, 虫垂炎, 感染症, 消化器がん, 胃十二指腸炎などの患者のほか, 正常人にも出現していることが報告されている.
Stratton and Rentonは, 大腸菌の菌体多糖体を吸着した赤血球は, 正常ヒト血清によって凝集するので, Springer らが見出したB型活性を持つE. coli O₈₆の菌体抗原が或る環境のもとで生体内で血球に吸着すれば, 血液型が変る可能性があり, acquired B は B-like 菌体多糖体が血球に吸着したものであることを示唆した.Springer and Ansellは, Isokiらによって見出された B型活性抗原多糖体をもつ腸内細菌を含めて, S.berkeley O₄₃, Arizona O₂₁, E. coli O₄, E. coli O₇, E. coli O₈, E. coli O₅₅, Proteus mirabilis, Proteus vulgaris Citrobacter, Klebsiella, Bethesda-Ballerup, Pasteurella pseudo-tuber-culosis, Herrrella 等のB型活性物質及び E. coli-O₈₆ から純化した lipopolysaccharide と protein-lipopolysaccharide を吸着したA及びO型赤血球は汎血球凝集性をもつようになるが抗B抗体を吸着解離することを明らかにした.この菌の多糖体吸着血球の汎血球凝集性は, 生後6～12ヶ月以上の殆んどすべてのヒト血清中に存在する抗E. coli抗体によるとし, B型ヒト血球から吸着解離した抗B 抗体は E. coli O₈₆ 多糖体の吸着0型ヒト血球を凝集することをみている.また, Marshは, T賦活性細菌の培養濾液がOやA型血球に pseudoB抗原の産生能があることを報告している. Gerbal らは, acquired B がA型のヒトにだけ出現することは, A型の抗原決定基のN-acetylgalacto-samine のdeacetylation が考えられるとし, さらに, acquired B は細菌の doacetylase が A1 型の抗原決定基である N-acetylgalactosamine に作用して, galactosamine を形成したものであり, この糖が坑B抗体と交叉反応するという仮説を提出した。そして, 患者からの acquired B 血球をアセチル化するとB様活性は消失し, Marcusら, Yamamo-to and IsekiにょりN-deacetylaseを含むことが明らかにされている Clostridum tertium Aの培養濾液を A1 型血球に作用させると, B 様活性が出現することを証明した.
ここでは, Clostridum tertium O (H), Le^aから精製したN-deacetylase 処理 A₁ 型血球及び型物質と, これらと反応するヒト血清中の凝集素との反応性について報告する.

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The chemical structure of growth hormone-releasing hormone (GH-RH) is not disclosed yet because of some difficult problems on purification. One of the most troublesome problems is the lack of sensitive and specific in vivo-assay method for GH-RH. Therefore, this study was attempted to search for 1) a more sensitive assay method using rats, and 2) a new reference standard as GH-RH instead of crude hypothalamic extracts (SME).
Adult male or female rats weighing 180-250 g were used in all experiments. Hypothalamic lesion was placed in some rats, which medial hypothalami were completely destructed by using a modified Halász-Pupp knife. Urethane or sodium pentobarbital was used as anesthetics. Reserpine or/and Estrogen + Progesteron was given subcutaneously prior to experiments in an attempt to increase the sensitivity of an assay method. Samples in physiological saline were administered intracarotedly once or twice, or were given intravenously through the jugular vein. Blood samples were taken at the various times from the jugular vien, or were collected from trunk by decapitation. Plasma GH, LH and prolactin were determined by the double antibody radioimmunoassay.
The following results were obtained;
1) Urethane anesthesia was able to lower plasma rat GH levels to 0 ng/ml for over 4 hours, and was suitable for GH-RH assay, whereas pentobarbital anesthesia gave the opposite results.
2) Reserpine pretreatment was advisable to increase the sensitivity of GH-RH assay. Using reserpene + urethane-rats, the minimum effective dose of GH-RH was 3 rat SME when injected intracarotedly.
3) The pretreatment of Estrogen + Progesterone or repeated administration of SME gave no benefit of GH-RH assay.
4) Prostaglandin E₁ was able to stimulate the release of OH and prolactin in the hypothalamic lesioned rats as well as intact rats. This means that Prostaglandin E₁ can act directly on the pituitary gland. This strong stimulator of GH release could be useful as a reference standard for GH-RH on establishing the more sensitive GH-RH assay system.
5) Similar results with 2) and 3) were obtained by using PGE 1 instead of SME. the maximum concentration of plasma GH and prolactin reached 5 minutes after PGE₁ injection. Therefore, it should be tried to collect blood samples 5 minutes after sample injection in GH-RH assay.
Thus, the present investigation has shown a newly-established, improved in vivo assay system for GH-RH.

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Groups of female Donryu rats were inoculated intraperitoneally with 1×10⁷ Yoshida ascites sarcoma cells. Animals were then subjected to the daily treatment with subcutaneous administration of water soluble prednisolone (P). Injection of P was initiated 2 days after inoculation in Group I, while Group II started to receive treatment immediately following inoculation. Each Group was further devided into three subgroups of A, B, and C, to which 1 mg, 0.2 mg, and 0.05 mg of P per 100 g of body weight were administerd, respectively. The result were summerized as follows :
1) Growth rate of the tumor cells was significantly suppressed in A and B rats of Group I and in all subgroups of Group II. This initial suppression, however, was transient and was followed by a rebound increase in the 5th or 6th day after inoculation.
2) On the 4th day, when the cell growth was still suppressed, both mitotic index and ³H-TdR pulse labeling index of the tumor cells were significantly decreased in all animals treated with P. The duration of mitosis remained unaffected, being 1 hour. Although no difference was observed in DNA synthetic time among the rats of Group I, it was significantly prolonged in Group II. G₂ duration was 2 hours in both P-treated and control rats. Thus, treatment with P appeared to prolong the duration of G₁ phase proportionally to the dosis administered. Compartment size of non-proliferative cells was found to be enlarged significantly, when the growth rate was retarded by the treatment.
3) In Group II, in vivo ³H-uridine labeling index was significantly depressed on the 4th day, while the mean grain counts were nearly equal to the control. However, the amount of ³H-uridine incorporated into RNA fraction of tumor cells was significantly less in P-treated rats than in the control. Thereafter, the incorporation increased in the P-treated rats, and decreased in the control. On the 5th day after inoculation, no significant difference became appearant between P-treated and control rats.
4) It was suggested that P mainly affects the pre-DNA synthetic phase of Yoshida ascites tumor cells and inhibits the initiation of DNA synthesis. These effects were weak, though definitive, and seemed to disappear within several days despite the continued administration.

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The indirect inguinal hernia in childhood often appear within the newborn period, but rarely come on the surgical theater during first 28 days of life.
There have been 411 children subjected to herniorraphy for indirect inguinal hernia at the Department of Surgery I., Gunma University Hospital during the 20 year period from April 1957. Of these, only five were newborn infants and all of them were operated upon because of the presence of an incarcerated or strangulated hernia. This is partly because of our principle that the operation is delayed until the infant weighs over 6 kg, unless symptoms of incarceration or strangulation develop.
This is a case report of one of these newborn infants.
The patient was a 18-day-old boy. He had recurrent bouts of bile stained vomiting and melena since the 13th day of life. The scout film of the abdomen on the 18th day of life showed distention of small bowel gas with air-fluid levels, characteristic signs of distal obstruction. Physical examination revealed an incarceration of the left-sided inguinal hernia, but the film taken before the admission showed a small accumulation of gas over the right ishio-pubic area (arrow) indicating the right-sided inguinal hernia. Surgery disclosed the patency of both sides of vaginal process and the strangulated but not gangrenous terminal portion of the ileum which was not resected. The patient recovered uneventfully and discharged on the 12th postoperative day.

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A house wife, 40 year old, had a delutional idea in relation to the entrance-examination of her second son, who failed in the examination. She thought that her first son had been persecuted by his classmates because he entered in the high school through backdoor. She felt deeply that she was responsible for it. Her idea developed to commit suicide or family suicide.
She was cured relatively shortly after admission to our hospital and intensive medication.
The background of her delusional thought, immature personality, environment, physical conditions and the meaning of intesive medication which brought recovery, were discussed.

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