Noninvasive recording of His bundle activity from the body surface by using the signal averaging was attempted in 20 cases of atrioventricular block and 6 cases of atrial fibrillation. As described in the previous report, the R wave in the standard electrocardiogram was used for triggering the signal averaging.
An obvious His bundle activity (G potential) could be recored by noninvasive method in 17 cases of AV block and 3 cases of atrial fibrillation. The G pottential was clearly seen by signal averaging 50, 300 and 400 cardiac cycles in first and third degree AV block and atrial fibrillation respectively. The simultaneous appearance of the G potential with the H deflection in routine His bundle electrogram was confirmed in 6 cases. Three cases of third degree AV block, in which the noninvasive recording of the His bundle activity was not successful, were confirmed to be H-V block by routine invasive examination. The reproducibility of the G potential was excellent in 2 cases, in which the reexamination was performed.
The G potential was obvious in only one or some of the precordial leads in several cases. Therefore, multiple leads across the chest should be performed in order to record the potential. Multiple leads were also necessary for precise determination of the onset of the ventricular activation, although the anteroposterior lead usually yielded the earliest QRS complex.
The noninvasive recording of His bundle electrogram is safe and useful diagnostic method for determination of the site of block in atrioventricular conduction disturbance, although our present recording method is of limited value, because the recording of the G potential is theoretically impossible in second or third degree H-V block.

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Mouse leukemia cell (L1210) is sensitive to 6-mercaptopurine (6-MP) and does not usually grow in medium containing 0.1 μg/ml of 6-MP. However, cells which could grow in medium containing 0.3 μ/ml of 6-MP were easily obtained at a rate of about 10^-5.
There were two types of 6-MP resistant strains thus obtained in 0.3 pig/ml of 6-MP; the one was low-resistant strain which could grow in 1 μg/ml of 6-MP but could not in 3 μg/ml, and the other was high-resistant strain which could grow even in 30 μg/ml of 6-MP. Most (96.1 %) of the resistant cells obtained in medium containing 0.3 μg/ml of 6-MP belong to high resistant strain and 3.9% to low-resistant one.
The resistance mechamisms of these two strains were not clarified but might be different from each other by the following results;
1) Low-resistant strain could grow in HAT medium but high-resistant strain could not.
2) The sensitivity of low-resistant strain to 6-MP was increased by the treatment with Levamisole, whereas no change was found in high-resistant strain.
3) Reversion to sensitive cell could be observed in the low-resistant strain but not in high-resistant one.
4) Only high-resistant cell could obtained from the revertant cell but no low-resistant cell could be obtained.

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We have checked the medical situation of the Maebashi Red Cross Hospital on children's medical problems covering the period of two years. That is before and after the installation of the Maebashi Night Emergency Clinic. We have found the number of patients to be on the decrease after the installation of the Clinic. For example, before the installation there were 472 patients and after the installation there were 348 patients.
Looking at the age bracket we found the largest number to be infants during the periods both before and after the installation of the Clinic. Looking at the days of the week, there was no remarkable distinction between days either before or after the installation. From the standpoint of the hours of the day, the number of patients for early morning (0-8 A.M.) was 12.3% before the installation and 21.3% after the installation. This was quite an increase. Two thirds of patients received medical care only once or twice both before and after the installation.
As to the diseases (before and after the installation), more than half were respiratory diseases. The digestive sickness came in two types. One was periodic vomiting and the other was gastroenterocolitis. The first, vomiting sickness, was on the decrease and the second was on the increase. As to other types of sicknesses we could not find any significant differences.
As to the early morning patients we could find no significant difference in regard to the number of patients for any types of diseases. As to the rate of early morning of inpatients there were 0.64% before and 1.15% after the installation.
The number of patients that were admitted was 7.2% before and 17.0% after the installation. This was found to be a remarkable increase. As to the patients sent from the Night Emergency Clinic these were comparatively very sick people.

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Plasma lysosomal enzyme activities were determined in 55 shocked patients and 36 patients received open heart surgery under extracorporeal circulation. Plasma β-glucuronidase in shocked patients depicted a higher value than control one in unshocked patients. Mortality rate of the patients increased parallel to the elevation of plasma concentration of the lysosomal hydrolase. However, 47 percent of shocked patients failed to show a release of the enzyme into the circulating blood. It was observed that β-glucuronidase and cathepsin-D in plasma elevated significantly after 60 minutes of extracorporeal perfusion. These results suggest that lysosomal hydrolases release into circulation from ischemic region during shock and hypoperfusion state.

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The present study was undertaken to determine the contribution of pancreas as a source of plasma lysosomal enzymes released during shock. In part one, mongrel dogs were subjected to splanchnic ischemia shock by the temporary ligation of superior mesenteric and celiac arteries. Plasma levels of β-glucuronidase had been raised during ischemia and reached a 4.4 times of sham operated control values in the terminal stage after release of ligation. Pancreatectomy being performed just prior to arterial occlusion failed to yield those significant rise of plasma β-glucuronidase. Diversion of thoracic duct lymph during ischemia also suppressed the increase of plasma lysosomal enzyme. In part two, Dogs which had received chronic ligation of pancreatic ducts for the atrophy of exocrine glandular cells were led to hemorrhagic shock according to the Wiggers' technic. There were significant differences in plasma lysosomal hydrolase levels between ductligated and control groups after 120 minutes of oligemic hypotension.
Those findings lead to a conclusion that lysosomal enzymes released into circulating blood during shock originate primarily from ischemic pancreas.

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