1) In the two patients of conjac asthma, on which I reported in the previous paper with regard to scratch test, ocular test (conjunctival test) and Prausnitz-Küstner test, etc, I succeeded to induce asthmatic paroxysm with the conjac flying-powder or its extract.
2) Asthmatic paroxysm could not be induced with the conjac flying-powder in any of eleven patients of general asthma. Nor could it be induced in either of the two patients of conjac asthma by the inhalation of powdered root of Colocasia antiquorum and spinach, etc.
3) The evident paroxysm could not be induced in the two patients by subcutaneous injection of histamine or mecholin (β-methyl-acetylcholine), but it could be induced in both by the inhalation of acetylcholine, and in case 2 by the inhalation of histamine.
4) Analysis for the allergen by the inhalation test revealed that it was found in the protein fraction
5) Administration of cortisone and ACTH could not prevent the development of paroxysm.
From these results, conjac asthma was considered to be allergic asthma due to the allegen of the conjac flying-powder

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A case of cryptococcal meningitis of a 32-year-old Japanese female was reported. The diagnosis of this case was determined only by histological examination after autopsy.
She died about a half year after se complained of headache as initial symptom.
1). Pathologically. A grayish, gelatinous, small nodule irregural in shape was found at the subpleural part of the right upper lung. There was thickening an i opacity with a bloody appearance of the meninges over the basal and occipital surface.
2). Microscopically. In the lung a granulomatous lesion was disclosed being composed of lymphocytes, mononuclears, multinucleated giant cells with dense fibrosis. There was no leucocytic element. Numerous cryptococci were presented in the necrotized center of this lesion.
3) From the histopathological findings, it would appear that death resulted from cryptococca meningitis spreading from primary focus in the lung.

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1) Two true aortic aneurysms which destructed the vertebrae were observed in an autopsy case of a 62 years old male, who died of apoplectic cerebral hemorrahge.
2) These aneurysms were found at the posterior wall of the descending thoracic aorta and of the abdominal aorta, respectively, and bath were saccular and fist-sized. The thoracic aneurysm was adhered to the bodies of the 5th, 6th and 7th thoracic vertebrae, and the abdominal aneurysm to the 11th, 12th thoracic and 1st lumbal vertebrae. In the both regions, the adherent vertebral bodies were markedly destructed and the posterior wall of the aneurysms was defected, so that the bone marrow of the vertebral bodies were exposed to the lumen of the aneurysms. Rupture of the aneurysms was not seen.
3) Histologically, marked syphilitic mesoaortitis and arteriosclerosis of the aorta were noticed, and both of them caused the aneurysms.
4) In the intima of the aorta, not only old arteriosclerotic lesions such as intimal hyalino-fibrosis, but also early changes, that is, intimal edema, swelling, fibrinoid degeneration, and dissolution of the newly formed elastic fibers and of calcium deposits, were noticed.

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Habu's (Trimeiesurus flavoviridis) bite shows two main local morbid changes : Hemorrhage caused by the venom and necrosis of the muscles. By animal experiments we discovered that these morbid changes were nothing but a digestive action of proteinase, which was contained in the venom of, Habu. We, the members of the Pathological and Microbiological Institutes, also made it clear that this proteinase-activity could be prevented when we cheleted Mm·· with EDTA, which is a component of this enzyme.
I have tried a therapeutical experiment of Habu venom in animal, using EDTA-Ca as cheleting substance.
Experimental animals (guinea-pigs) were given 0.5mg (dry weight) venom subcutaneously. Immediately after the injection, EDTA-Ca was injected by incision. Hemorrhagic necrosis (a characteristic venomous effect of Habu venom) was repressed.
It is true that antivenom serum therapy is quite effective for Habu venom therapy, but when this therapy is not available in the places like mountain sides or fields where the bites often occur (and that is the case we often meet), it would be advisable to take this compound as first-aid in the Habu-prevailing districts.

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A 45-year-old man was admitted complaining of headache, pain in the neck, lumbago, dizzines and progressive weakness of one and a half year's duaration. Examination revealed enlargement of s the liver, hyperproteinemia (9.6mg/dl) due to increased γ-globulin (39.0%), Bence Jones proteinuria and multiple pounched out areas cf bone destruction in the skull, ribs and ileum. He died on the 57th hospital day.
Autopsy disclosed multiple nodules or areas of bone destruction disseminated in the scull and ribs. Microscopically, the nodules were composed of loosely packed cells with moderate pleomorphism growing solid sheets, which were characterized by oval shape, excentrically placed rounded nuclei, fairy abundant cytoplasms of dark blue tint, its Pyronin affinity and perinuclear pale areas, having nuclear and cytoplasmic characteristics similar to those of tissue plasma cells. Extramedullary tumor cell infiltration was observed in lymphnodes, liver and spleen. Hyaline casts and calcific deposits were noticed in the distal part of the tubules of the kidneys with associating foreign body reaction. The condition was summarized as plasma cell myeloma.

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Neurosecretory phenomena in the adults of Bufo vulgaris formosus and Rana nigromaculata under the normal and the experimental conditions were studied histologically.
Two kinds of the neurosecretory activities in the cells of the preoptic nucleus could be demonstrated which seems to be responsible for the production of different kinds of secretory material, for these neurosecretes the terms of “A substance” and “B substance” were given by the present anthor as follows.
One of the secretory activities is concerned with the production of fine granular secretes (B substance) which are stained with aniline blue component of Mallory's triple stains and chrome-alume hematoxylin (CH) in Gomori's stains, rejecting iron-hematoxylin. As the results of the histological observations, it could be mentioned that the preoptico-hypophysial tract served as a pathway for this neurosecrete into the neurohypophysis. Another kind of neurosecretory material (A substance) was in the form of large or small spherules and showed trong affinities for acid fuchsin, iron hematoxylin and CH.
Production of “A substance” is pronounced remarkably in the female toads in the breeding season and become feeble in summer. When the animals were brought in the cold circumstance in summer, there were observed considerable increase of the secretes.
A series of experiments in which various kinds of drugs were injected to the hypophysectomized frogs showed that the cells of the preoptic nucleus were stimulated to produce “A substance” by Anteron, Prehormon and Progynon. Under such a condition, “A substane” which was released directly into the interstitial spaces, passed over the optic chiasma and reached at the tissues of eminentia interpeduncularis where it was discharged into the blood circulations. On their way to the eminentia interpeduncularis, “A substance” loses the stainability for CH and gains for phloxine.
As to the results of the experiments mentioned, some considerations were made concerning the possible physiological influences of “A substance” on the gonadotrophic activity of the adenohypophysis.

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An association between hypertension and body build, based on the data of 516 adults in a farming population of Gunma Ken, was studied. An assessment of the body build was carried out with the “Wetzel Grid” technique. Furthermore, physical measurements were analyzed by several indices, such as skin-fold at iliac spine, circumference of abdomen and chest. Our findings disclosed the fact that the male subjects rated as A₄ and those of larger abdomen circumference of both sexes were more hypertensive. However, these hypertensive subjects were only one fifths among total hypertensive population. Thereore the preventive approach by means cf “weight control” in public health practice is not fully efficient, although some fractions of over-weight and obese subjects are more hypertensive.

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The characteristic symptom of the staphylococcal infection is the infiltration of polymorphnuclear leucocytes in the inflamatory area. Menkin reported the permeability factor (i.e. leucotaxine) which was extracted from the exsudate of inflamation. But the new substance which promotes the leucocytic migration is discovered in St. aureus or in the culture filtrate of St. aueus.
In this paper the assay method of L.P.S. (the substance which promotes leucocytes migration) was described.
Intracutaneous injection of St. aueus was followed by the skin resection, and the rcsected skin was crushed. The extracted leucocytes from crushed skin were counted by the ordinary method.
Another method for the determination of L.P.S. was adopted. The subcutaneous air cyste was followed by the injection of the fractions of St. aueusr. The conditions for the assay of L.P.S. were desecribed.

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By the Cabaud method, SGOT was determined chiefly with inpatients in the Shibusawa surgery clinic and experimental animals.
In acute myocardial infarction, SGOT remarkably increased, attaining the maximum at 24 hours. In fresh infarction, SGOT value was proportional with the extent involved by infarction, and above 200 units indicated unsatisfactory prognosis.
Concerning hepatic diseases, up to 2, 000 units was shown in hepatitis, 20, 000 units in intoxication, and 300 units in regressive degeneration. In carbon tetrachloride intoxincation, SGOT value was approximately proportional with in jury of hepatic cells. Histologically, correlation between centrilobular zonal necrosis and SGOT was confirmed. SGOT is a good index for the degtee of destruction of hepatic cells. The administration of 200 mg of cortisone was highly effective for chronic hepatitis, rapidly restoring SGOT to the normal value.
When the elevation of alkaline phosphatase level was due to bone disease, SGOT did not rise, but when it was due hepatic disease, SGOT rose.
In extrahepatic occulusivc icterus, SGOT showed slight rise. When it was surgically removed, SGOT returned to the normal value in 12 weeks.
SGOT can be used for the differentiation between icterus, liver cirrhosis, liver tumor and extrahepatic occulusive icterus.
There is gross correlation between surgical operation and SGOT. In the operation of the chest, cardiac vessel, and bile duct, and in severe wound, remarkable rise in SCOT was observed. Excepting these cases, only slight and transient rise of SGOT was noted.
Rise in SCOT was observed in experimentally produced infarction of the heart, lung, spleen, intestine, kidney and liver.
Relation of SGOT to g-6-pase, aldolase, amylase, alkaline phosphatase, blood ammonium and serum irou was sought.
Determination of liberated glucuronic acid was performed after the Ishidate-Nakajima's method using ion exchange resin, In hepatic diseases, bound glucuronic acid in blood showed greater increase than free one.

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The flight muscles of insects have a high α-glycerophosphatedehydrogenase activity, and according to Kennedy and others α-glycerophosphate is one of the components of α-lecithin in its biosynthesis.To throw some light on the metabolism of lipids in muscle, the author has studied the oxidation and dehydrogenation of lecithin especially in bee flight muscle comparing with muscles of rat, frog and sparrow. The results were as follows :
1) Addition of egg lecithin to the homogenate of bee flight muscle increases the rate of respiration in the presence of DPN by 10-15% and in the presence of methylene blue by 25%. The addition of egg lecithin to the homogenate of rat femoral muscle, however, does not increase respiration.
2) In the presence of NaF (or iodoacetate) and methylene blue, the addition of lecithin increases the rate of respiration of the homogenate of bee flight muscle more than twice as much as in the case without the addition of lecithin.
3) The muscles of bee, rat, frog and sparrow have been found to possess dehydrogenase activity both soy-been lecithin and egg lecithin. Among these muscles, bee flight muscle has the highest dehydrogenase activity upon both lecithin varieties.

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There are two kinds of substance or substances which react positively to mercuric-iodide reaction (i. e. prevent the color change of yellow mercuric-iodide precipitation to red one) : one is colloidal and the other is non-colloidal. The rate of excretion of the former is estimated by method A and that of the latter by method B of the method modified by Yamazoe and his associates. The results of the present investigation were as follows :
1. The values of the method A, as well as that of B. are low where urine specimens are taken immediately after the subject gets up in the morning, keep a relatively high level during the daytime and decrease during the night. The change of urine volume per unit time hardly affects the value of the reaction.
2. As effect on the reaction of surgical operation (six cases of thoracoplasty and two cases of pulmonary resection) the values of method A showed a distinct elevation, maximum value being at third or forth day after the operation and restored to the preoperation level about a week after the operation. The value of method B also showed a marked increase, maximum being at first or second day and restoration about a week after the operation.
3. The values of the reaction in normal adults (twenty male subjects) were compared with those in patients with pulmonary tuberculosis (eight light, fifteen intermediate and eight severe cases). No difference was found between the normals and patients with light and intermediate cases, but patients with severe cases showed distinctly higher values than the former in method A as well as in method B. No correlation was found between erythrocyte sedimentation rate and the value of the reaction even in patients with severe pulmonary tuberculosis.

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1) Ether inhalation anesthesis and chemical hibernation in cases with hepatic disorder produced remarkable chauge in serum protein fractions.
2) In preerserved blood, with the prolongation of days of preservation, decrease in Al and increase in γ-Gl were shown.
3) Effect of blood transfusion on serum protein began to appear immediately after it, and around 20 hours, improvement in blood protein picture was observed. Thereafter it gradually returned to the pre-transfusion value. In the case of cancer, this improvement was not satisfactory despite sufficient amount of transfused blood, and the return to the pre-transfusion value was more rapid.
4) Dialysis with the artificial kidney on cases with renal insuffiency produced decrease in α₂-Gl and increase in γ-Gl, which is closely related with hepatic disorder. Decrease in lipoprotein was due to blood transfusion and heparin, used as anticoagulant.
5) Change in serum protein picture in cancer was dependent on the site of its development, the state of its advance, hypotrophy, and hepatic function, and not on cancer itself.
6) Hepatic function and change in serum protein fractions after the operation showed close similarity, and had great bearing on determining serum protein picture.

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Survey was carried out on medical cares for open tuberculous patients at home in the jurisdictoin of Shibukawa Health Center, the discovered at collective medical examinations before one year, and consequently with relatively short histories, and similar patients in the jurisdictions of 5 Health Centers in Gunma Prefecture, previously registered, and consequently with relatively long histories of the disease. In this survey, various statistical tables were prepared from the view points of the public health and economical conditions, and the following conclusions were drawn :
1. Those receiving medical cares were more public health minded than non-recipients, and desinfection of sputum and isolation were carried out more perfectly with the formers.
2. Rate of receiving medical cares had significant positive correlation with the presence or absence of socialized medical cares, and with their degree.
3. As to economical living conditions of the patients, there were problems with the subresponsible persons for the household economy and the group of low income (annual income of 80, 000 to 120, 000 yen). Namely, they had less opportunity for social security benefit, and further had various difficulties in receiving medical cares.
4. More than 10% or almost 20% of patients receiving medical care were non-eligible for the social security benefit paying all the cost of medical cares. Of the patients receiving medical cares by the social security system, 70% were afforded the benefits under social insurance, and 30% by the livelihood protection law. The rate of medical care recipients among all the patients eligible for the livelihood protection was almost 100%. Only a few eligible for the livelihood protection received medical cares at the onset of the disease, but the number of recipients conversely increased at about 4 or 5 years after the onset, which is the end of period of indifference, as reported by the author in the previous paper.

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In the previous papers, it was reported that immunization of mice with living bacterial vaccine (attenuated S. enteritidis strain) was followed by strong active immunity to challenge infection with virulent strain 116-54. But the immunization of mice with killed bacterial vaccine was also effective to some extent to the challenge infection of S. enteritidis. Sixty per cent survival occured when vaccine and challenge dose were administered intraperitoneally. However, mice were not so resistant to intravenous challenge when vaccine was administered intraperitoneally. This facts suggested that some of immunity observed mightbe “immunity owing to the local resistance” in abdominal cavity.
Dr. Hazato reported the effectiveness of intravenous administration of killed vaccine.
In the present paper, bacteriological and histopathological studies of the intravenous administration of killed vaccine were described. It was confirmed that the effectiveness of the immunization with killed vaccine varied with the combination of the routes of administration of vaccine and challenge infection. And mice were most resistant to intraperitoneal challenge when vaccine was administered intraperitoneally. The nodules formed in the liver, spleen, and other reticuloendotherial systems after receiving killed vaccine, are qualitatively different from true typhom caused by the injection of living vaccine.

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1) Patients with stomach cancer showed high rate in positive xerogelfigure, and besides, the procedures of this test is extremely simple. On account of these, the test is highly valuable as a supplementary diagnostic method for stomach cancer.
2) For those with negative xerogel-figure, the rate of stomach cancer was extremely low.
3) Positivity rate of this test was higher than that of the Matsubara's carcinoreaction in stomach cancer. When used in combination with the latter, this test will become still more valuable as a supplementary diagnostic measure for stomach cancer.
4) Many of patients with stomach cancer and positive xerogel-figure showed increase in serum mucoprotein. Further, when mucoprotein was added to normal serum, its xerogel-figure turned positive. Consequently, serum mucoprotein is considered to play an important role in determining the feature of the figure.

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1) The xerogel-figure test was carried out with patients with lung cancer, and the positivity rate was found to be 73.6%. The negativity rate for pulmonary tuberculous patients was 88.2%.
2) The X-ray examination combined with the xerogel figure test will lower the rate of missing lung cancer. This test has advantage in the simplicity of its procedures, especially in mass screening.
3) In patients with lung cancer and positive xerogel figure, increase in serum mucoprotein was observed.

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1) Many of patients with hepatogenic jaundice showed negative xerogel-figure, while those with obstructive jaundice showed positive figure.
2) The xerogel-figure and serum mucoprotein were in parallel relation both in hepatogenic and obstructive jaundice. In differentiating these two form each other, the xerogel-figure can substitute for serum mucoprotein.

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According to the studies by Akamatsu and Furiya, the author devised a method for the determination of serum peptidase using metaamino benzoic acid peptide as substrate.
The method : 0.1cc of serum was mixed whit veronal buffer and M /400 glycylamino benzoic acid, kept at 37°C in a water bath for 1 hour, and deproteinized with 5% trichloracetic acid.
The separated metyamino benzoic acid was subjected to diazo reaction with Tsuda reagent to develop color, and examined with the electophotometer. The optimal acidity for serum peptidase was pH 7. Its action was inhibited by cobalt ions and manganese ions, but not by magnesium ions. Its normal value ranged 1418%.
The results : The rate of hydrolysis of peptidase was decreased in anemia.

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In the first report, the author described the method to determine the peptidase value with 1 cc of serum. In the present, he reports on the method of the determination with 0.1 cc of serum.
The method : 0.1cc of serum was mixed with veronal buffer and M/2000 dl-Leucyl amino benzoic acid (orM/400 glycyl amino benzoic acid) keep, at 37°C in a water bath for 1 hoar, and depro teinized with 20% trichloracetic acid. The separated metyamino benzoic acid was subjected to diazo reaction with Tsuda reagent to develop color, and examined with the electrophotometer. The optimal acidity for serum peptidase was pH 7. Its action was inhibited by cobalt ions and manganese ions, but not by magnesium ions.

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Serum peptidase level was compared with the results of various tests of hepatic function, and it was found that positivitiy rate of glcyl-m-aminobenzoic acid was higher than that of alkali phosphatase, and that of dl-leucyl-m-aminobenzoic acid was lower.

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1) Experiments were performed on the fusion of bacteria using Escherichia coli K-12, its biochemical mutant, W-1177, E. coli C₂, slightly different from W-1177 in antigenic structure, CW-25, produced by the fusion of W-1177 and C₂ and having the antigens of both, and Fstrains, produced from CW-27, K-12, and C₂ by removing F f actor with acriflavine. The f requency of the fusion was approximately 1 out of 10-10.
2) Some of Strains produced by fusion possessed the antigens of one of the parent strains, and the others possessed the antigens of both. The latter had, besides both parents' antigens new specific one. The sugar-fermenting abilities were combinations of their parents' ones in all of them regardless of the above mentioned difference in antigenic structures.
3) Since in the majority of cases the sugar-fermenting ability of the fused strains was nearer to that of F^-, it is generally considered that part of the ability of F⁺ would have appeared in the order of the arrangement of genes on the chromosome
4) The gene for the development of the specific antigen of K-12 was designated as W, that of C₂ as C, and their allelomorphs as W, w and C, c. And using these, the genetic mechanism underlying the development of the antigen was investigated. The gene of the antigen may be separate from nutritional requirement, SM resistance and sugar fermentation, not having direct connection with these. But if there is any connection, it will concern the locus. Namely, the gene of the antigen can be considered to be found between the loci of nutritional requirement for T and L and SM-resistance.

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One hundred percent of 5 week-old ddN mice died within 10 days after being inoculated by the intraperitoneal route with 0.5 ml containing 10^-7mg (300cells) of S. enteritidis strain 116-54.
But 5 week-old mice were completely resistant to the challenge infection of 10^-5 or 10^-4mg of the attenuated S. enteritidis strain SER. One hundred percent of 5 day-old mice died within 5 or 6 days after being infected with 10^-7 or 10^-8mg of the attenuated S. enteritidis strain SER. The resistance of mice to the infection of S. enteritidis varied with the age of mice after birth.
Immunization of mice with 10^-5 mg of attenuated S. enterilidis strain SER and with 10 ⁷ mg of virulent S.cnteritidis strain 116-54 was followed by strong active immunity to challenge infection with virulent strain.
One hundred percent of mice survived infection with 100 fold 10^-7mg of virulent strain 116-54 and twenty percent of mice survived infection with 100 thousand 10^-7 mg of virulent strain.
The body weight of mice immunized with living attenuated strain SER decreased rapidly within two days after being infected with 10^-2 or 10^-4mg of virulent S. entcritidis strain and recovered accompanying with the increase of body weight.
But the body weight of mice immunized with living vaccine did not decrease after being inoculated with 10^-5 or less amount of virulent S. enieritidis strain 116-54.

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