An aluminium silicate suspension was infused into the carotid of dogs, and clinical Symptoms and blood distribution and histological change in the brain were investigated in the course of time. Soon after the infusion, flaccid or spastic paralysis occurred on the opposite side of the infusion, or on both sides. At this time, general congestion was observed in the brain. With the lapse of time, the congestion increased, became uneven, and from about 30 minutes, ischemic foci appeared. Change in nerve cells became conspicuous, as the blood distribution became uneven and ischemic foci appeared, but this did not coincide with the appearance of clinical symptoms. The development of clinical symptoms under the present experimental conditions is considered to be closely related with the general congestion. After 1 hour, an intravascular substance which reacted metachromaticly with thionin was observed around a particle of aluminium silicate. This substance became greater with the lapse of time, and is considered to play a part, a s thromboid substance, in bringing about the uneven distribution of blood, and pathohistological changes in the brain. However, the appearance of this substance was transient, and after 75 hours, it was no more observed.

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Experiments were carried out with normal primigravidas and multigravidas from the later stage of pregnancy to puerperal time, and the following results were obtained :
1. Circulating acidphil count was determined by the Landolph's method with 5 primigravidas and 3 multigravidas, and it was found that in each case, the count tended to lower from the later pregnancy to the time of parturition, and to rise thereafter. Further it was found that in the primigravidas, it tended to lower gradually as parturition drew near.
2. Urinary chemocorticoid was determined by the Nakao-Aizawa's method with 13 pregnant, 9 parturient, and 7 puerperal cases, and it was found to increase in the later pregnancy, to increase still more in parturition and on the first day of puerperum, and then to decrease.
3. Urinary 17-ketosteroid was determined by the Holtroff's (extraction) and Drekter's (colorimetric) method with 5 cases of first pregnancy, and it was found to change nearly in parallel with chemocorticoid. Further, the determination by the Adachi's method revealed that, at parturition, fraction II abruptly decreased, IR, M and increased, and IV and V slighly increased, and that thereafter N and V abruptly increased and IV and MI decreased.
4. From these observations, it was concluded that parturition can be regarded as a stress.
In Patients with various gynecological diseases Urinary 17-ketosteroid was extracted by the Robbie-Gibson's method, and determined by the Callow's method, with the following results :
1. The value was abnormally high for one of 17 cases of ovary dysfunction, and was low for 2 cases of the same. It was generally low for those of small gonadtropin excretion, and it had no definite parallel relation with urinary estrogen. For cases of recurrent amenorrhea, it was low within 6 mont hs, and later tended to rise to the immediately above the normal range.
2. The value was found rather high for 2 cases of primary sterility without organic abnormalities.
3. The value was normal for 3 cases with cervical cancer, and it was rather low for 4 cases of uterine myoma.
4. When serum gonadtropin from a pregnant horse was administered for 4 consecutive days, urine 17-ketosteroid value increased remarkably at 34 days following the administration, but then decreased again.
5. When 19-norsteroid was given to women of amenorrhea and normal menstruation, no change was observed : but when 19-norsteroid was given in association with estrogen to amenorrheal women, decrease in urinary 17-ketosteroid was observed.

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1. In rabbits with chronic uterine fistula, estrogen was found to enhance oxytocin sensitivity of uterine muscle, and progesterone conversely to depress it.
2. The relation between automatic uterine movement and oxytocin sensitivity was pursued with rats by the Magnus method, and the following results were obtained : (a) Uterine movement of non-pregnant rats was most rhythmical in oestrus with high oxytocin sensitivity; (b) in early pregnancy, oxytocin sensitivity of the uterus temporarily lowered, but gradually rose from the middle through the late pregnancy, attaining the maximum in the time of labor; (c) when estrogen was administered in late pregnancy, oxytocin sensitivity was raised to such chdegree as in labor; (d) when the animals were pretreated with progesterone, labor was not induced even after the time of the expected period, and the administratione of estrogen could not immediately produce such particular oxytocin sensitivity as in labor; (e) this particular enhancement of oxytocin sensitivity in labor is considered to have important bearing on the induction of labor, and estrogen is assumed to act as a cause of this enhancement.

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1) The constituting peptides of the protein obtained by dilute sulfuric acid extraction from defatted healthy lung tissues and caseosus lesions, have been studied.
2) On the basis of counter current distribution study of the performic acid oxidized DNP-proteins of the original samples could have separated into several single peptides.
3) Qualitative analysis of the N-terminal residues together with that of constituting amino acids of the individual single peptides, have shown ultimately, that the constituting peptides of the protein of the healthy lungs were : aspartyl, alanyl, and leucyl peptides in contrast to the aspartyl, beta-alanyl, and histidyl peptides from the caseosus focci respectively.

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In the previous paper it was reported that Staphylococcus aureus produced the substance promoting the leucocytes migration. And the experimental conditions and method were discussed. In the present paper, the experimental conditions for the purification of L. P. S. was described. The production of L. P. S. by Staphylococcus aureus Terajima, Staphylococcus aureus K. 363 and E. coli was compared. K. 363 was most eminent in the production of L. P. S. and leucocytes migration reached maximum after 4 hours of the injection of K.363. There was no difference in L. P. S. between living and killed bacteria. But the L. P. S. curve with time in the injection of bacteria was qualitatively different from the curve in the injection of glass powder or physiologic saline solution. The possibilty to extract L. P, S. was discussed.

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In the previous paper it was reported that immunization of mice with living attenuated S. enteritidis strain SER was followed by strong active immunity to challenge infection with virulent strain 116-54. And one hundred percent of mice survived infection with 100 fold 10^-7 mg (usual challenge dose) of virulent strain 116-54. The present investigation is an attempt to elucidate the relation between S. enteritidis and the abdominal monocytes of the mice. Monocytes were obtained from the abdominal cavity of normal and immune mice which had been given an intraperitoneal injection of glycogen solution as hemotactic agent four to five days prior to the collection. Cells were washed twice by centrifugation and then resuspended in Hanks's solution.
The attenuated strain SER of S. enteritidis was used for living bacterial vaccine and chrome-alum vaccine for killed bacterial vaccine.
The results were as follow :
1) The phagocytic activity of monocytes derived from mice immunized with killed bacterial vaccine was most active. And the activity of monocytes derived from normal mice was greater than that of monocytes derived from the mice immunized with living bacterial vaccine.
2) There was on difference in the number of bacteria ingested in the monocytes derived from normal or immune mice.
3) The monocytes derived from the mice immunized with living bacterial vaccine inhibited the intracellular multiplication of bacteria without regard to the presence of antibody.

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In the previous papers it was reported that immunization of mice with 10^-5 mg of living attenuated S. enteritidis strain SER was followed by strong active immunity to challenge infection with virulent strain 116-54. But killed bacterial vaccine was also effective against the infection with virulent S. enteritidis. Sixty percent survival occured when vaccine and challenge dose were administered intraperitoneally.
In this paper immunization of mice with virulent and attenuated S. enteritidis strain killed by ultra-violet irradiation was investigated.
The results were as follows :
1) If the logarithm of the surviving fraction was plotted against time of irradiation, points lie on a exponential curve. After 20 minutes irradiation, the surviving fraction decreased to the range of 10^-7 of the original cells (10 ), but ultraviolet-irradiation was not able to kill completely the living cells.
2) The cell suspension irradiated with ultra-violet light contains living and killed cells. But the UV irradiated cell suspension has not so high immunizing effect against the infection of mice with S. enteritidis. When the mice were immunized with most effective dose of UV irra diated strain, mice were found in the carrier state. So it is suggested that the effectiveness, of UV irradiated strain may be caused by living cells containing in the UV irradiated strain.
The mice immunized with killed cells of UV irradiated strain were not so resistant to the challenge infection of virulent S. enteritidis, when compared with chrome-alum vaccine.
3) When the mice were injected with killed cells of UV irradiated strain, so-called faomy cell change of the vaccine nodule and numerous vaccine nodules were observed in the peripheral area of the liver.
But the accumulation of fatty acid in the vaccine nodule were not observed.

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The author has studied some biochemical and morphological properties of mitochondria of several kinds of muscle, chiefly giant mitochondria of flight muscle of dragon fly. The results were as follows;
1) Giant mitochondria of flight muscle of dragon fly were situated on the A band region of myofibrils, and neither A substance nor I substance could be found in the myofibrils.
2) Giant mitochondria of flight muscle of dragon fly were able to oxidize the intermidiate metaboliates of the tricarboxylic acid cycle. Their ATPase was not activated by DNP and usually they had no latent ATPase. However, in the fresh mitochondria isolated as quickly as possible, DNP-activation of ATPase and latent ATPase could be observed.
3) Maximum magnesium-activation of ATase of giant mitochondria was obtained at a concentration of about 10^-3M of MgCl₂. CaCl₂ slightly inhibited mitochondrial ATPase.
4) Mitochondrial and microsomal ATPases isolated from bee flight muscles were distinctly different from each other in regard to the effect of MgCl₂ and CaCl₂. Therefore it seemed apparent that there exist two different kinds of particulate ATPases in the muscle and they have different functions.

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The authors have studied the effects of various ions and other factors upon the contraction of melanophore of cat-fish skin induced by acetylcholine, with a viw to its utilization for microdetermination of acetylcholine. The decrease of ATP in the course of rigor mortis was also observed. The results were as follows.
1) Melanophores of cat-fish skin contract by addition of acetylcholine to the soaking medium (0.7% NaCl) at a concentration higher than 0.01μg/ml but are not influenced by various ions at pH 5-7.
2) If eserine sulfate (10^-6M) is added to the medium to inhibit the activity of acetylcholinesterase, tissue extracts do not influence the contraction of melanophores by acetyl-chol ine and it is possible, to determine approximate coetnnt of acetylcholine of various tissues e xtracting it by 0.78% NaCl which contains 10^-6M eserine sufate.
3) When ATP content in the skin of cat-fish drops below 70% of initial value, melanophores begin to contract, and at a ATP content of 20-30% of initial value the contraction is complete.

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Previously I reported on the effect of autonomic and mandibular neurotorny on the development and calcification of rabbit dentine. In the present, I observed the effects of stimulants and blocking agents of autonomic nerve. There are reports on the effects of autonomic nerve toxin on the development of the tooth, but many points are still in obscurity. I applied the Okada-Mimura's vital staining method with lead acetate, by which, change in the speed of formation and development of dentine and variation in the stripes figure of calcification can be observed in tissue; slices. At the same time, serum Ca level was determined.

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Since the Warburg's method was established for the investigation of metabolism in the irradiated tissue. many reports have been published, but the results are not always in agreement.
As to the cause of the discordance, it can be considered that various may secondarily be produced during irradiation. In the present experiments, effects of roentgen irradiation on Yoshida sarcoma, which is wandering single cell, were investigated with the Warburg's manometer, and the following results were obtained.
1. The respiration of Yoshida sarcoma was slightly inhibited by irradiation in adose of 5700 r (11%). In the case of endogenous respiration, increase was sometimes observed (below 600 and above 950 in 1 mm³).
2. The metabolism of aerobic sugar decomposition was more affected than respiration (24.6%)
3.Anaerobic sugar decomposition was most sensitive, being remarkably diminished with change in the, dosage of irradiation.

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1) It was found out that in antigenic transformation of Salmonella group E by epsilon 15 phage, and that of group B dy iota phage, strains, undergoing the transformation, followed by mitoses and multiplications, are lysogenized by the respective phages, producing them, while those not undergoing the transformation are not lysogenized, not producing them. Further, when the transformation was carrid out with the phage combined with radioactive isotope P³², radioactivity was stronger in those undergoing the transformation than in those not undergoing it.
From these facts it was assumed that the antigenic transformation by the phage would be different from the transduction of characters conveyed by the phage, but a change produced by the genetic material contained in the phage itself.
2) Anti-15 factor serum did not inhibit so much the multiplication of strains of subgroup E₁, containing somatic antigens 3, 10, but had strong activity to inhibit the multiplication of subgroup E₂, containing 3, 15. On the other hand, anti- epsilon 15 Phage, prepared with pure epsilon phage did not exert so much inhibitory action on the strain having antigens 3, 15. Further in experiments with the antibody combined with I¹³¹, it could not be demonstrated that neither the anti-15 antiboody nor the anti-epsilon 15 phage antibody could enter the cell body.
These facts seem to indicate that in antigenic transformation of Salmonella group E by anti-15 factor serum, in which from a strain of subgroup E₂ is produced by mutation a strain of subgroup E₁ through the loss of epsilon 15 phage, the factor serum would have at least selective action.

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From Oct. 1953 to Novenber 1958, thoracoplasty was performed upon 18 cases with pulmonary tuberculosis patients over 50 years of age, at Maebashi Prefectual Sanatorium.
The patients were divided into 10 moderatly advanced and 8 far advanced pulmonary tuberculosis, and the mean of age at operation was 52 years of age.
Examination for tubercle bacilli gave negative results in about all cases of thoracoplasty, but there were two cases in which liver disturbance still remained after operation, of the whole patients showed in an average about 2, 3kg decrease in weight as compared to that of before operation.
We should treat surgically the old tuberculosis patients of good condition whom chemotherapy and antibiotics could not care.

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The A. P. of N. ishiadicus which was separated from a toad was recorded by means of monopolar or bipolar lead, and then the distance between two electrodes was changed on each experiment.
By monopolar lead, the configuration of A. P. led at a central part is large and abrupt (Fig. 8 a), and that at the peripheral is small and gentle (Fig. 8 d). By bipolar lead, if the distance of two electrodes is short, the configuration of A. P. is small, but if the distance is long, it is very large and a plateau. between two deflections appears.
This configuration of A. P. (bipolar lead) may be able to be composed of each A. P. of monopolar lead, considering its distance and direction.
Some results of the first reports which inquired into the nerve were proved logically, by means of the Model (The Yamagiwa-Lillie's and the Matumoto' s Model)

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As experimental materials we used solutions of dyes belonging to tryphenyl methan, azo anthraquinon. cyanin, thlazin and nitro groups, which were irradiated with the following results :
The colors of aqueous and chlorform solutions of these dyes faded or changed when irradiated with X-ray, the latter being sensitive more markedly. This can be attributed to the indirect action of Cl-base which is produced from chloroform. Cyanin and aminoazobenzene dyes, which showed especially remarkable change, can be used for determing the chemical dose of the ray below hundreds of roentgen.

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1. The extermination of Oncomelania nosophora (Robson) by means of a soil-burning flame thrower is a very effective method provided that the use and operation are appropriate. The standard method for ordinary geographical terrain is 4-minute buring per 5m², promising termination of more than 80 per cent.
If deposits and stones are not found in the area to be cleaned, 2-minute combustion per 5m² can provide a sufficient effect. On the other hand, when the area contains many stones destruction by fire for 6 minutes or more per 5m² is necessary. In the case of the area containing flowing water or swamp, the effect of this method is not promising at all.
2. The destruction rate reaches its maximum on the third day after the soil burning.
3. From the economic point of view, the performance of this method is comparatively expensive, therfore, it should be used as an adjunctive of other principal method of Oncomelania extermination.

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This investgation was based on 200 biopsy materials concerning the breast examined histopathologically at our department in 1954 and 1955.
The histological diagnoses as follows : Mastitis 13, mastopathia 100, fibroadenoma 30, intraductal papilloma 1, carcinoma 47, gynecomastia 6, and others 3.
Among 13 cases of mastitis, 4 cases of plasma cell mastitis were noticed. Three of them had been clinically diagnosed as mammary carcinoma.
There were cases showing simultaneously the picture of mastopathia and fibroadenoma. In some of mastopathia the histologic picture simulated to that of fibroadenoma was also noticed.
Concerning the histological change in the ground substance and the fibrosis of stroma;, some differences were noticed between mastopathia and fibroadenoma.
The 31 per cent of the cases of breast carcinoma were accompanied with mastopathia, but a histological picture showing the canceration of mastopathia was not observed.

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The essential substance responsible for the reaction (a kind of colloidal reaction) modified by Yamazoe was studied. The results obtained were as follows :
1. Acid mucopolysaccharides, which may be present in urine, such as chondroitinsulfuric acid, hyaluronic acid and heparin, were not shown to be responsible for the reaction.
2. A mucoprotein fraction, which was precipitated by the addition of several times the volume of the alcohol to the urine specimen, showed a positive reaction, if its concentration was made higher than in normal urine. However, since in our method a highly diluted urine specimen is always used, the mucoprotein fraction also could not be regarded as responsible for the reaction. Also, Südhof's mucoprotein was responsible neither for this reaction, nor for Donaggio reaction.
3. Blood group-specific substance in urine had also no relation to the reaction, because it was easily precipitated by alcohol in contrast to the mercuric iodide reaction-positive substance.
4. The evidence of paper electrophoresis of the urine pigment, which was soluble in glacial acetic acid, lead us to the conclusion that urochrome is the essential substance involved in the reaction.

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When the mice were immunized with 10^-5 mg of attenuated strain and with 10^-7 mg of virulent strain of S. enteritidis, mice obtained the strong immunity against the challenge infection of virulent strain of S. enteritidis. And mice survived from the infection with 100 fold ordinary challenge dose of virulent strain. Attenuated strain is quite important to give strong immunity with living vaccine. But it is immunologically quite difficult to identify the attenuated strain SER. It was the purpose of this experiment to identify the effective strain of SER for living vaccine.
Phages were obtained from natural sourses and classified into three groups S-phage, SER-phage and R-phage. By using these phages, it is quite easy to classify S. enteritidis into S and R type. And it is also possible to determine the situation of SER between S and R type and to know the immunogenic properties of SER strain by phage typing.

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The thyroidal uptake of radioiodine was observed after oral administration of I¹³¹ for 72hours.In hyperthyroidism, the maximum uptake was botained at 3 or 6 hours after I¹³¹ administration and I¹³¹in thyroid decreased at 24 hours, after I¹³¹adrninistration.
In simple goiter, this phenomenon was not observed and the peak of the thyroidal uptake was seen at 24 hours.
The uptake was higher in hyperthyroidism than in simple goiter (higher than 30% at 1 hour, higher than 45% at 24 hours), and in hypothyroidism, the uptake was always lower than 10%.But the high uptake was observed in a few cases of simple goiter.
At early time (1 or 3 hours after administration of I¹³¹), the blood level of I¹³¹was higher in simple goiter than in hyperthyroidism, but in the latter, plasma I¹³¹ level decreased again 24hours after I¹³¹ administration.
This phenomenon was not observed in the former.
Thus the Blondal index (PI¹³¹ at 48 hours/PI¹³¹ at 2 hours) noticed no overlapping.
The protein bounded I¹³¹ increased also after 24 hours in hyperthyroidism and the conversion ratio to protein bounded I¹³¹ was higher than in simple goiter.
The metabolism of I¹³¹ in hyperthyroidism was changed by phenobarbital medication and resembled the type of simple goiter.
By the treatment with methylthiouracil etc, I¹³¹ metabolism was normalized, but after improvement of I¹³¹ metabolism, a few relapse was occured.

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The author investigated effects of chemicals in different concentrations on anaerobic metabolism of Ehrlich'S ascitic cancer cells and the mechanism of chemical protectin against the administration of them before and after irradiation.
1) Conditions of irradiation : 180 kvp, 25 mA, 20 mmAl, 16 em, 1073 r/min., 500 r, 1000 r, 2000 r, 3000 r, 4000 r, 5000 r, 6000 r ; ih the case of concomitant administration of chemicals, 6000r.
2) Gas : During irradiation, N₂ or O₂ was used, which was exchanged with 5% CO₂+N₂.
3) Method of determination : The routine method was followed with the warburg' S manometer.
4) Concomitant use of chemicals : NaCN, cysteine, glata-thione were used as KRB solutions in concentrations of 3 ×10^-1-M, 3 ×10^-2 M, 3×10^-3 M, 3×10^-4 M.
Results : Oxygen result was observed.
when NaCN, cysteine, and glutathione were used separately, metabolism was enhanced, but when they were used concomitantly, effect of NaCN to increase sensitivity, qrotective effect of cysteine, and inverstion of effect of glutathione by difference in the concentration of effect of glutathione by difference in the concentration were observed.

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