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Volume 56, Issue 4
Displaying 1-22 of 22 articles from this issue
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2007 Volume 56 Issue 4 Pages 174-176
Published: April 01, 2007
Released on J-STAGE: October 14, 2011
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Kazue KURIHARA2007 Volume 56 Issue 4 Pages 177
Published: April 01, 2007
Released on J-STAGE: October 14, 2011
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Naoki TANAKA2007 Volume 56 Issue 4 Pages 178-181
Published: April 01, 2007
Released on J-STAGE: October 14, 2011
JOURNAL FREE ACCESSMolecular chaperones suppress the protein aggregation to assist the protein folding. In this study, we have investigated the application of the chaperone peptide, derived from the substrate binding site of the molecular chaperone αA-crystallin. αA-crystallin is one of the major structural proteins within the eye lens, and exhibits chaperone-lik e activity to be involved in the maintenance of the lens transparency. Former study has shown that a synthetic peptide KFVIFLDVKHFSPEDLTVK, which corresponds to the residues 70-75 of αA-crystallin exhibited the chaperone-like activity [K. K. Sharma, et al. J. Biol. Chem., 275, 3767 (2000)]. In addition, the chaperone-like activity was improved by the K70D substitution in this peptide (mini-αA-crystallin). We have constructed an expression vector for the luciferase joined with mini-αA-crystallin by PCR technique, and the chimerical luciferase was produced by in vitro protein expression system PROTEIOS (TOYOBO). However, the expression efficiency and the thermal stability of the chimerical lucifrease were not improved compared with wild type luciferase. Alternatively, we immobilized mini-αA-crystallin on the surface of the nano-particle using affinity tag, and examined the effect on the amyloid fibril formation, which causes the neurodegenerative disorder. The chaperon-like activity of mini-αA-crystallin was significantly improved by the immobilization on the nano-particle, suggesting that it would be applicable for various fields including the protein engineering and medicine.View full abstractDownload PDF (1935K) -
Kiyotaka SHIBA, Ken-ichi SANO2007 Volume 56 Issue 4 Pages 182-186
Published: April 01, 2007
Released on J-STAGE: October 14, 2011
JOURNAL FREE ACCESSWe isolated peptide aptamers against the surfaces of titanium and carbon nanomaterials. Here, we show applied researches of these peptide aptamers in nanobiotechnology field. TBP-1 is the peptide aptamer against titanium. We found that the peptide binds to Ag and Si as well as Ti. Furthermore, the peptide mediates mineralization of TiO2, SiO2, and Ag. Thus, TBP-1 is a bifunctional peptide, i. e., it is a binder and a mediator for mineralization. By utilizing this unique character of TBP-1, we have established a novel method of nanofabrication, in which binding and mineralization by a peptide aptamer are alternately utilized to assemble multilayered nanostructures comprised of metal loaded cage proteins ornamented with Ti-binding peptides.View full abstractDownload PDF (3548K) -
Shigeki SASAKI2007 Volume 56 Issue 4 Pages 187-190
Published: April 01, 2007
Released on J-STAGE: October 14, 2011
JOURNAL FREE ACCESSRecently, important roles of non-canonical higher order structures of DNA and RNA have been identified in regulation of gene expression. Therefore, the molecules that can target specific DNA and RNA sequences will be useful not only as innovative bio-tools but also as potential candidates for new therapeutic agents. Triplex helix DNA formation with the use of synthetic oligonucleotides (triplex-forming oligonucleotides; TFO) provides a unique method for recognition of duplex DNA leading to inhibition of transcription with high sequence specificity in the antigene method. However, as the stable triplex DNA is formed only with the duplex with homopurinehomopyrimidine sequence, development of TFO as the biological tool has been hampered. This limitation arises from the fact that pyrimidine bases provide one hydrogen bonding site to natural bases within the major groove, and pyrimidinepurine base pairs interrupt triplex formation. Much effort has been devoted to overcome to this problem, and some successful approaches were recently reported with the use of newly designed nucleoside analogs. The author's group also succeeded the development of original nucleoside analogs for the recognition of pyrimidinepurine interrupting sites. This mini-review focuses on the development of nucleoside analogs for the formation of stable triplex DNA.View full abstractDownload PDF (1751K) -
Ichiro HIRAO, Michiko KIMOTO2007 Volume 56 Issue 4 Pages 191-195
Published: April 01, 2007
Released on J-STAGE: October 14, 2011
JOURNAL FREE ACCESSThe base pair formation of AT and GC in DNA is a fundamental rule of storage and expression of the genetic information. The creation of a new base pair, compatible with the natural ATan d GCb ase pairs, enables the expansion of the genetic alphabet and code for the site-specific incorporation of unnatural, functional components into nucleic acids and proteins. Recent progress of the development of unnatural base pairs that can function in replication, transcription, and translation is opening the door to novel biotechnologies.View full abstractDownload PDF (1966K) -
Atsushi OHTA, Hiroshi MURAKAMI, Hiroaki SUGA2007 Volume 56 Issue 4 Pages 196-199
Published: April 01, 2007
Released on J-STAGE: October 14, 2011
JOURNAL FREE ACCESSThe translation apparatus is sophisticated machinery that polymerizes amino acids to polypeptide according to the genetic triplets encoded on messenger RNA (mRNA) sequence. Because of this property, ribosome generally acts as a superb template-directed polymer synthesizer and therefore translated polypeptide shows narrow distribution of molecular weights with complete controlled sequence. Unfortunately, this superior system is limited to polypeptide synthesis because of the lack of genetic code which converts genetic triplets to any monomers except for 20 natural amino acids. To expand the application of ribosome, we utilized the concept of genetic code reprogramming to assign several codons as hydroxy acids and performed mRNA-templated polyester synthesis. We have demonstrated the sequence and length of polyesters can be controlled by the mRNA sequence as designed. This is a novel technology for the synthesis of polyester and polyesterpolypeptide copolymer for the investigation of new materials.View full abstractDownload PDF (1707K) -
Yasuhiro AOYAMA2007 Volume 56 Issue 4 Pages 200-203
Published: April 01, 2007
Released on J-STAGE: October 14, 2011
JOURNAL FREE ACCESSGene coating with glycocluster nanoparticles derived from macrocyclic glycocluster amphiphiles affords size-regulated“glycoviruses”which are transfectious. This neutral, i. e., non-cationic glycoviral gene delivery is not only novel as such but also provides a basis to reveal the significance of the size factor free from the charge factor. Cationic liposomes which are readily fusible upon interaction with DNA can be made infusible upon ceramic coating of the surface. Analysis of transfection activities of neutral glycoviruses and cationic cerasomes (ceramic-coated liposomes) in a viral size allows to establish a structure-activity correlation for the otherwise complicated gene delivery processes which are governed by such many factors as charge, size, and receptorligand interactions usually in a difficultto analyze manner.View full abstractDownload PDF (3143K) -
Osamu KANIE2007 Volume 56 Issue 4 Pages 204-208
Published: April 01, 2007
Released on J-STAGE: October 14, 2011
JOURNAL FREE ACCESSCombinatorial oligosaccharide synthesis became possible at last. Despite the increasing biological interests, this has been a challenging and difficult task. Efforts to simplify the entire synthetic protocol resulted in mixtures of anomers, individual compounds in which were isolated by HPLC in the end. The importance of having synthetic combinatorial library is the possibility of finding novel structures those mimic the function of natural counter receptor. These compounds might be useful as pharmaceutical seeds. Furthermore, it is considered that physical data contained in these compounds are useful in the structural elucidation method using mass spectrometry. This account tries to introduce some of the successful synthesis of a combinatorial oligosaccharide library and the use in mass spectral investigation.View full abstractDownload PDF (2104K) -
2007 Volume 56 Issue 4 Pages 209
Published: April 01, 2007
Released on J-STAGE: October 14, 2011
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2007 Volume 56 Issue 4 Pages 209a
Published: April 01, 2007
Released on J-STAGE: October 14, 2011
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2007 Volume 56 Issue 4 Pages 209b
Published: April 01, 2007
Released on J-STAGE: October 14, 2011
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2007 Volume 56 Issue 4 Pages 209c
Published: April 01, 2007
Released on J-STAGE: October 14, 2011
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2007 Volume 56 Issue 4 Pages 209d
Published: April 01, 2007
Released on J-STAGE: October 14, 2011
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2007 Volume 56 Issue 4 Pages 209e
Published: April 01, 2007
Released on J-STAGE: October 14, 2011
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Yutaka AMAO2007 Volume 56 Issue 4 Pages 211
Published: April 01, 2007
Released on J-STAGE: October 14, 2011
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Eiji WATANABE2007 Volume 56 Issue 4 Pages 212
Published: April 01, 2007
Released on J-STAGE: October 14, 2011
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Toshihiro IHARA2007 Volume 56 Issue 4 Pages 213
Published: April 01, 2007
Released on J-STAGE: October 14, 2011
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Yoshiko MIURA2007 Volume 56 Issue 4 Pages 214
Published: April 01, 2007
Released on J-STAGE: October 14, 2011
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Takuro NIIDOME2007 Volume 56 Issue 4 Pages 215
Published: April 01, 2007
Released on J-STAGE: October 14, 2011
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Hiroyuki KATAYAMA, Fumiyuki OZAWA2007 Volume 56 Issue 4 Pages 216-220
Published: April 01, 2007
Released on J-STAGE: October 14, 2011
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Shohei MAKITA2007 Volume 56 Issue 4 Pages 221
Published: April 01, 2007
Released on J-STAGE: October 14, 2011
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