OKAMOTO, one of the authors, investigated the amino acid contents in the crystalline and non-crystalline regions separated by means of the diluted HCI hydrolysis method, and concludedthat there could not been observed the differences in contents of both regions. Whiletwo components of silk fibroin were reported recently by COLEMAN, DRUCKER and AKABORI etal successively. But the relation between the two regions and the two components is complicatedand not clear, so that the authors tried to clarify this complicated relations and gotthe following results.
Silk fibroin of domesticated silkworm (
Bombyx mori) and wild silkworm (
Antheraea pernyiand
A. yamamai) was dissolved in strong hydrochloric acid or in 85% phosphoric acid, andthen regenerated by neutralization, and these processes were repeated several times. Atevery process the regenerated fibroin was gathered and hydrolysed completely with 6 N HCI, and the hydrolysates were concentrated and devoted to the paper chromatographic method, and amino acids varieties composing of each fractions were detected and compared eachother.
In all cases the amino acids varieties of both fractions, corresponding to the regenerateand non-regenerate components, were fairly different.(Fig. I, Ia, II) MURASE reported that the crystalline region of the wild silkworm separated by means ofthe diluted HCI hydrolysis method was composed of such small molecular amino acid asglycine, alanine and serine. But we could not separate from the wild silk such region asabove mentioned with the same method.(Fig.III, IV)
Judging from the above mentioned facts, it might be concluded that the two componentsof fibroin are not the same with the two regions of it, and asumably the two or more componentsof fibroin intermingle in both regions and are not observed the distinct differences incomponents of both regions.
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