The antitumor antibiotic, Neocarzinostatin (NCS) was isolated by Ishida et al. from the culture filtrates ofStreptomyces carzinostaticusF-41. The antitumor activities of NCS have been demonstrated against various mouse and rat ascitic and solid tumors and clinical applications of NCS have been shown to be effective against acute leukemia, stomach cancer, pancreatic cancer, bladder cancer and oral cancer. The purpose of this study is to examine the localization of NCS in oral tissues by means of enzyme-labeled antibody method. The experimental methods were as follows, anti-NCS antibody was made by immunization with NCS against rabbits and anti-NCS IgG was prepared by DEAE cellulose column chromatography. Labeling of horseradish peroxidase to IgG and preparation of tissue specimens were followed by Nakane's method. The findings were as follows; (1) In oral region, NCS was distributed highly in squamous cell epithelium of oral cavity and it had a tendency to be affinitive to gland tissues of salivary glands and it was detected highly and sebaceous glands, too. (2) The distribution of the drug was little in lymphatic tissues such as the spleen and the thymus but it was detected highly in gland tissues of the gastro-intestinal tract and the pancreas. In the kidney, NCS was detected very highly in renal tubules. (3) Against ascitic Sarcoma 180 cells, NCS adheared to the membranes of tumor cells within 5 minutes after injection and disappeared after 4 hours.
The purpose of this investigation is to analyze the relationship between craniofacial pattern and intermaxillar disharmony in cases of anterior cross-bite at IIA stage. The materials consisted of standardized lateral head roentgenograms and study models taken from 51 children who had anterior cross-bite and 25 children who had good occlusion. Anterior cross-bite samples were divided into two groups, by the value of ANB angle, group A (ANB≤O; 14 children) and group B (ANB>O: 37 children) . After the comparison between 3 groups on 90 measurements, the factor analysis was employed in this study on 25 measurements which reveal structure of craniofacial complex, jaw relation, total teeth size and arch length size. In control group and group A, five factors were extracted which acounted for 76% (control) and 78% (group A) of the total variance in each groups, and these were identified as; I) craniofacial rotation, II) total facial hight and depth of midface, III) mandibular morphology, IV) teeth size and arch length size, and V) intermaxillar relationship. And then four factors, in group B, which acounted for 60% of the total variance was extracted. In group A, ANB and over-jet showed high factor loadings on factor I, and cranial base angle (NSBa), one of variances which reveal craniofacial rotation, was significantly smaller than that in control group.
In an attempt to clarify the metabolic characteristics of condylar and tibial epiphyseal cartilage matrix in the organ culture, the materials taken from a few day-old neonatal rabbits were cultured in the Ham F-12 medium modified by Nakamura. Histochemical and Autoradiographic studies and Biochemical Analysis using35S-sulfate showed the following results. 1. The increase in35S-Activity of the condylar and epiphyseal cartilage continued for the first 24 hoursin vitro. The activityin vitrowas about 500 of thatin vivo. 2. When the35S-Activityin vivoandin vitrowere compared, the epiphyseal cartilage showed a closer resembalance than the condylar cartilage. 3.35S-Activity increased remarkably since 12 hours after35S administration in our organ culture. 4. The greatest number of grains was present in the transitional zone in the condylar cartilage and the deeper proliferative zone in the epiphyseal cartilage. 5. Some of the hypertrophic chondrocytes, traditionally thought inactive, revealed marked35S-sulfate uptake. 6. 94-96% of acidic mucopolysaccharides of the condylar and epiphyseal cartilages consisted of chondroitin sulfate A and C, bothin vivoandin vitro.
For the purpose of studying the vascular permeability of periodontal membranes in relation to tooth movement, monophasic stimuli were provided by putting 0. 20, 0.13 and 0.08 mm thick bands on the upper first molars of rats. Horseradish peroxidase was used as a tracer to examine the permeability histochemically. The results are as follows: 1. In periodontal membrane compressed to less than 2/3 of the original width: 1) The permeability increased immediately after tooth movement and reached its maximum after 3 and 6 hours. 2) After one day the area with the increased permeability was observed at the periphery of the so-called anoxia tissue. After 5 days it appeared at the periphery of the necrotic tissue and the granulation-like tissue. 3) On the control side the permeability also increased in the first 6 hours. 2. In the periodontal membrane not compressed to less than 2/3 of the original width faint and transient increment of the permeability appeared, and no remarkable tissue changes were noted. 3. On the tension side the permeability was always weaker than that of the pressure side. 4. The mechanism of the enhancement of the permeability during the first 6 hours seemed different from that of one and 5 days.
There is a few investigations on the conditions of males, females with OPA attachments and abutment teeth. The subjects were 103 cases followed up for about these seven years, including 192 abutments with OPA attachments which have been applied to the few remaining teeth in overlay dentures and removable partial dentures. Methods 1) Male parts were observed on the Standarized photograph. 2) Female parts with O'ring rubber were observed by taking apart from the denture. 3) Abutment teeth were investigated about the X-ray photograph, tooth movility, pocket and infiamation of the marginal gingiva. Conclusion 1) Only 5.2 % of 192 abutments were extracted, but other cases followed-up examinations have been in almost good conditions. 2) The proportion of the female parts of dentures that had fractured was 28.80 of resin-based dentures and 18.3 % of metal-based dentures. 3) On the almost casting males except neck part two types abrasion by denture resin have been observed. 4) On the lower part of O'ring rubber the five types wears have been observed, while on the upper part very little abrasion. 5) The proportion of enlargement of inner diameter of O'ring rubber was 39.2 0. The enlargement was often seen in bucco-lingual direction. 6) A highly significant correlationship between the enlargement of inner diameter of O'ring rubber and denture movement was seen.
The purpose of this study was to examine the interrelation between the morphological and the physiological changes induced by rapid palatal expansion. The subjects were consisted of 20 orthodontic patients without any TMJ dysfunction. A path of habitual jaw movement and EMG were recorded with a sound of occlusal contact at the time before and after expansion. The results obtained are as follows: 1. Most of the subjects showed an increase of the sagittal difference of habitual jaw movement at the level of 5 mm from the intercuspal position. 2. Changes of EMG pattern were characterized by the loss of co-ordination among the jaw closing muscles, and also the silent period, especially on its duration and latency, which were much varied after expansion. 3. After two months however, these variations of silent period tended to return to the stage before expansion. 4. It seemed that the occlusal changes induced by rapid expansion were affected not only by the amount of integrated afferent stimuli but also on its force distribution in the course.
The structure and characters of the transparent layer and adjacent layers of dentin of extracted human third molars having decay was investigated by microscopy, Knoop hardness determination, optical microscopy, microradiography and scanning and transmission electron microscopy, obtaining the findings below: 1. On the vertical sections of decayed dentin, the normal layer, the lightly transparent layer, the heavily transparent layer and the discolored layer were distinguished. 2. Deposition of platelike crystals on the dentinal tubule lumen walls was found starting from the uppermost part of the normal layer and filling up many of the tubules in the lightly transparent layer. 3. In the heavily transparent layer, most dentinal tubules were filled up with rhombohedral crystals resulting remarkable increase of X-ray opacity in the layer. 4. Knoop hardness was highest at the junction between the normal and lightly transparent layers and to some extent lower at the heavily transparent layer showing crystal decrease of intratubular layer and intertubular dentin due to carious lesion.
The materials used for this study were the normal enamel from freshly extracted human erupted permanent teeth. The structural features of dental cuticle were observed using light microscope, scanning electron microscope and transmission electron microscope. The preparations used for light microscopic observations were ground sections and sections decalcified with Kirino's decalcification method. The decalcified sections were stained with hematoxylin-eosin. The material used for transmission electron microscopic observations were cut from the surface layer of enamel using a dental diamond disc. The cubes of enamel were fixed and embedded with routine method. The ultrathin sections were cut nearly vertical and parallel to the enamel surface through the surface layer of enamel with diamond knife without decalcification. As the results, the dental cuticle was divided into two layers, the primary dental cuticle and the secondary dental cuticle. The primary dental cuticle was composed of enamel crystals and the secondary dental cuticle was composed of fine granular crystals. The dental cuticle was more resistant to acid than normal enamel, especially the secondary dental cuticle showed the highest resistance for acid etching.
We compared radiographically the results of endodontic treatment performed by Ingle's standardized technique with those done by non-standardized technique. The standardized and non-standardized technique groups included 187 teeth (289 root canals) and 216 teeth (292 root canals) respectively. Both two groups were treated by undergraduate students of the Faculty of Dentistry, Tokyo Medical and Dental University. The results obtained were as follows: 1) In the standardized technique group, the average distance between an obturating point tip and the radiographic root apex was 1.4mm short of the apex with standard deviation of 1.4mm. In the non-standardized one, it was 0.3mm beyond the apex with standard deviation of 1.2mm. There was a statistically significant difference between them (Student's t test, p<0.05) . 2) In the standardized technique group, an adequate seal was found in 235 root canals, a doubtful seal in 46 and a bad seal in 8. In the non-standardized technique group, an adequate seal was found in 90 root canals, a doubtful seal in 176 and a bad seal in 26. There was a statistically significant difference between them (chi-square test, p<0.05) .