Serum and gingival crevicular fluid (GCF) from the patients with periodontitis were examined for local immune responses against 4 periodontopathic bacteria. Immunoglobulin G antibody titers to the bacterial antigens were measured by the enzyme-linked immunosorbent assay (ELISA) . As bacterial antigens,
Bacteroides gingivalis, Capnocytophaga Ochracea, Actinobacillus actinomycetemcomitans and
Fusobacterium nucleatum were used. They were ultrasonicated and the soluble supernates were obtained as antigens. The GCF was collected from the maxillary and mandibular anterior regions of 10 patients. Some were allowed to collect the GCF from different regions. The ratio of the antibody titer of the GCF to the serum was calculated. The IgG titers against
B. gingivalis and
F. nucleatum showed statistically a significant correlation between the values of the serum and the GCF. The highest ratio was found in the IgG titer against
B. gingivalis, showing an average value of 0.80. However, the GCF IgG titers against
B. gingivalis and
A. actinomycetemcomitans showed sometimes different values in different regions of the same mouth. A positive correlations was not found between the GCF IgG titers and the various clinical parameters.
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