The purpose of this study was to investigate the hypergravity-induced responses and their mediators of osteoblastic cell line, MC3T3-E1. The synchronized G
1 or the S Phase cells were exposed to 5 and 18×g hypergravity at 37°C. The migration velocity was measured and the morphology was observed. MC3T3-E1 cells were cultured for 1, 2 or 3 days at 37°C, exposing to 5, 10, 20 and 40×g hypergravity. The proliferation, prostaglandin E
2 (PGE
2) production rate and alkaline phosphatase (ALPase) activity were measured. The results were as follows:
1) In the G
1 phase of the cell cycle, the migration of MC3T3-E1 cells was increased by 18×g. In the S phase, the morphology altered depending on the g-stress.
2) The proliferation of MC3T3-E1 cells was enhanced at 20 and 40×g but reduced at 10×g. The proliferation of HeLa cells and JTC-12 cells was also enhanced at 40×g.
3) Indomethacin (10
-5M) reduced the proliferation of MC3T3-E1 cells induced by 40×g. But indomethacin (10
-5M) did not reduce the proliferation of HeLa cells.
4) The increase of the released PGE
2 from the cells depended on the time (1-8h) and the gravity (1-40×g) .
5) The increase of the ALPase activiy of MC3T3-E1 cells also depended on the gravity.
These results suggest that the hypergravity enhanced the proliferation of MC3T3-E1 cells via PGE
2-mediated mechanism.
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