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Volume 62, Issue 1
Displaying 1-15 of 15 articles from this issue
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Tsuneo Tabata1995 Volume 62 Issue 1 Pages 1-15
Published: March 31, 1995
Released on J-STAGE: October 08, 2010
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Masako Ishii1995 Volume 62 Issue 1 Pages 16-28
Published: March 31, 1995
Released on J-STAGE: October 08, 2010
JOURNAL FREE ACCESSMandibular condylar cartilage is considered as one of the growth cartilages, but it repre-sents many distinct features. Collagens in cartilage matrix consist mainly of type II collagen but lack type I collagen. Recently it is reported that condylar cartilage contained both type I and type II collagens, while type I collagen was lacking in growth plate cartilages of adult rat tissue.
In order to investigate whether such difference in matrix collagen exists in the embryonic period, an immunohistochemical study of type I and type II collagen was performed on fetal mouse condylar cartilage and compared to the limb bud cartilage (tibial anlage) .
When the first appearance of condylar cartilage was recognized at the 15th day of gestation, reaction to anti-type I collagen was detected in the cartilage matrix, while reaction to anti-type II collagen was very faint. After the 16th day, the pattern of immunohistochemical reaction was almost the same as that after birth. These observations of fetal condylar cartilage were completely different from those of limb bud cartilage.
These results suggest that the abilities of the condylar cartilage and the limb bud to synthesize matrix collagen are different from the embryonic period.View full abstractDownload PDF (9501K) -
Yasuhiro Sato1995 Volume 62 Issue 1 Pages 29-47
Published: March 31, 1995
Released on J-STAGE: October 08, 2010
JOURNAL FREE ACCESSThe purpose of this research was to investigate influence on the sternocleidomastoid muscle (SCM) activity in clenching depending on body and head positions.
Seven male subjects without jaw-dysfunction were asked to exert maximum voluntary contraction in three body positions : sitting, supine and prone. As for the head positions, those of normal and full rotations (right and left) in each body position were required. In addition, in the sitting position, those of half rotation (right and left) were performed.
EMG activity was recorded by means of bipolar surface electrodes.
The results were as follows
1. In full rotated head positions, EMG activity of the contralateral SCM varied about twice as much as in normal head positions.
2. In half rotated head positions, EMG activity of the contralateral SCM varied 1.20 times as much as in normal head positions.
3. In rotated head positions, EMG activity of the ipsilateral SCM varied from 0.81 to 1.22 times as much as in normal head positions.
4. EMG activity of SCM without clenching (background activity) had little influence on that with clenching.
5. In normal head positions, EMG activity in the supine position varied 0.86 times as much as in the sitting position.
In conclusion, the head positions have much more influence on EMG activity of SCM in clenching than the body positions.View full abstractDownload PDF (2127K) -
—Radiotherapy Cases—Nobuyuki Tanaka, Kenichi Shionoya, Takao Miyamoto, Kensuke Nakano, Mas ...1995 Volume 62 Issue 1 Pages 48-55
Published: March 31, 1995
Released on J-STAGE: October 08, 2010
JOURNAL FREE ACCESSWe examined the ultrastructures of the tongue squamous cell carcinomas which received radiotherapy and compared the metastatic cases with the non-metastatic cases. The results were as follows:
1. The non-metastatic cases were clinically T1N0 or T2N0 and histologically Grade I or II of the WHO classification. Electronmicroscopic observation of these cases revealed that numerous microvilli and a small number of desmosomes were found and the cell attachment seemed to be weak. These findings were different from those of the non-metastatic cases which received surgery.
2. In the metastatic cases of which the primary lesions were controlled, microvilli were not developed and a number of desmosomes were shown as compared with those in the nonmetastatic cases. However, cell atypia and dispersion of the nuclear chromatin were clearly recognized.View full abstractDownload PDF (10007K) -
Sayuri Kikuchi1995 Volume 62 Issue 1 Pages 56-77
Published: March 31, 1995
Released on J-STAGE: October 08, 2010
JOURNAL FREE ACCESSIn order to elucidate the genetic effects on the growth and development of craniofacial regions, HIV-1 transgenic mice (TG mice) which exhibited the phenotype of anterior crossbites were investigated by various methods.
Northern hybridization showed that the introduced gene was expressed from 14.5 days post coitum to adult. In morphological measurements using digital converter “GRADICON”, significant differences between TG mice and wild type mice (WT mice) were found at 5 weeks of age (5W) for most measurement items. Especially at 7W, the results indicated remarkable reductions in maxilla and catch up growth in the mandible. On the other hand, the serum alkaline phos-phatase activity of TG mice (3W, 5W, and 7W) was significantly higher than that of WT mice, and also the low level of inorganic phosphate was detected at 3W for TG mice. These results suggested that TG mice had a disorder of bone metabolism.
Other TG mouse lines carrying the same DNA construct showed normal figures in the craniofacial regions, suggesting the possibility that the phenotype of anterior crossbites and the disorder of bone metabolism were not caused by functions of HIV-1 genome but by insertional mutation in craniofacial development.View full abstractDownload PDF (7512K) -
Satoshi Yamaguchi1995 Volume 62 Issue 1 Pages 78-93
Published: March 31, 1995
Released on J-STAGE: October 08, 2010
JOURNAL FREE ACCESSThe purpose of this study was to investigate molecular mechanisms of growth arrest of normal human epithelium. By using a primary outgrowth culture system of normal human ectocervical epithelial cells which undergo density arrest, several known genes and 3 unknown genes, whose expression is induced during growth arrest, were identified. In this report, we characterized one of these novel growth arrest Inducible genes, clone 6A1. The size of 6A1 transcript was 1.3Kb, and the isolated clone was 1, 356bp long and had a putative open reading frame that encodes 309 amino acids (34Kd), which was consistent with the results of in vitro transcription-translation.In normal human epithelial cells contact inhibition dramatically induced 6A1 expression but serum starvation induced little. And the expression of 6A1 was transiently induced in the early phase of growth arrest. In contrast to normal epithelial cells, normal fibroblast and 2 out of 4 carcinoma cell lines expressed little or no 6A1 mRNA either during contact inhibition or during serum starvation. These results suggested that 6A1 may be important in contact inhibition of normal epithelial cells and loss of its expression may play a role in tumorigenesis.View full abstractDownload PDF (8339K) -
-Expression of Type I Collagen and Bone Sialoprotein mRNAs-Sayaka Domon1995 Volume 62 Issue 1 Pages 94-105
Published: March 31, 1995
Released on J-STAGE: October 08, 2010
JOURNAL FREE ACCESSThe purpose of this study was to investigate expression of type I collagen and bone sialoprotein (BSP) mRNAs in the alveolar bone accompanied with tooth movement. The right side of the upper jaw of the rat was used for orthodontic tooth movement, and the left side was used for physiological tooth movement. After the proper number of days of movement, his-tological specimens were decalcified and sliced into paraffin sections. The expression of mRNAs was examined by the in situ hybridization method. In the samples of physiological tooth movement, a high level of expression in both mRNAs was observed in osteoblasts along the mineralization front and adjacent osteocytes in the interradicular septum (IRS) . In contrast, a low level of mRNA expression was observed on the opposite side of the IRS. In the specimens of experimental tooth movement, a high level of expression was detected in the osteoblasts on the tension side of IRS, but negligible reaction in those on the compression side.
These results suggest that BSP gene expression as well as that of collagen are related not only to mineralization in physiological bone remodeling but also to the process by activated osteoblasts induced by orthodontic force. In addition, response to the artificial force was observed in osteocytes in IRS.View full abstractDownload PDF (7955K) -
Hong Zhao1995 Volume 62 Issue 1 Pages 106-126
Published: March 31, 1995
Released on J-STAGE: October 08, 2010
JOURNAL FREE ACCESSGallium alloys that have been developed as a substitute for dental amalgam were investigated for fatigue properties and the effects of environmental factors were also examined.
Cylindrical specimens were fatigue-tested employing cyclic tensile loading. Statistical definition of the fatigue limit at 105 cycles was obtained according to the staircase method. The significance of environmental factors were evaluated by analysis of variance using the ortho-gonal table L8 (27) .
The fatigue limit of gallium alloys was higher than that of a conventional high copper amalgam and was influenced by the experimental environment such as temperature and water. The fatigue fracture occurred in the matrix and the interface between the matrix and the core.
Gallium alloys have excellent fatigue properties as a filling material.View full abstractDownload PDF (19363K) -
—Age-related Changes of Osteoblastic Cells from Mouse Mandible—Atsushi Yoshida1995 Volume 62 Issue 1 Pages 127-148
Published: March 31, 1995
Released on J-STAGE: October 08, 2010
JOURNAL FREE ACCESSTo investigate the age-related changes of mandibular bones, three osteoblastic cell lines (11-4tc, 32-3c and 84-3c cells) with a high level of alkaline phosphatase (ALPase) activity were isolated from the mandibular bones of mice at the three different ages (11, 32 and 84 weeks old) .
Growth rate, ALPase activity, mineralization ability and the expressions of mRNAs of collagenα (I) (CO) and osteopontin (OP) were investigated in these cell lines.
The effects of β-glycerophosphate, retinoic acid, lα, 25 (OH) 2D3 and estrogen on cellular activity were also examined.
The results were as follows
1. The doubling times of 11-4tc, 32-3c and 84-3c cells were 20, 16 and 39 hours respectively.
2. The maximum ALPase activity of 11-4tc, 32-3c and 84-3c cells in a confluent monolayer were 0.403, 0.020 and 0.035μ moles phenol/min/mg protein respectively
3. The mineralization ability and the growth response to estrogen were decreased with advancing age of the donor
4. The mRNAs of CO and OP were expressed in these cells. 1α, 25 (OH) 2D3 and retinoic acid increased the expression of mRNAs of OP when 11-4tc cells were cultured for 7 days and when 32-3c and 84-3c cells were cultured for 18 days.
These results suggest that the aging of the donor relates with the depression of proliferation, mineralization and biological responses to estrogen, 1α, 25 (OH) 2D3 and retinoic acid in osteoblastic cells in mandibular bones.View full abstractDownload PDF (22153K) -
[in Japanese], [in Japanese]1995 Volume 62 Issue 1 Pages 149
Published: March 31, 1995
Released on J-STAGE: October 08, 2010
JOURNAL FREE ACCESSDownload PDF (144K) -
[in Japanese], [in Japanese]1995 Volume 62 Issue 1 Pages 150
Published: March 31, 1995
Released on J-STAGE: October 08, 2010
JOURNAL FREE ACCESSDownload PDF (156K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese]1995 Volume 62 Issue 1 Pages 151
Published: March 31, 1995
Released on J-STAGE: October 08, 2010
JOURNAL FREE ACCESSDownload PDF (2058K) -
[in Japanese], [in Japanese]1995 Volume 62 Issue 1 Pages 152
Published: March 31, 1995
Released on J-STAGE: October 08, 2010
JOURNAL FREE ACCESSDownload PDF (144K) -
[in Japanese], [in Japanese]1995 Volume 62 Issue 1 Pages 153
Published: March 31, 1995
Released on J-STAGE: October 08, 2010
JOURNAL FREE ACCESSDownload PDF (166K) -
1995 Volume 62 Issue 1 Pages 156-174
Published: March 31, 1995
Released on J-STAGE: October 08, 2010
JOURNAL FREE ACCESSDownload PDF (2564K)
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