The present study was carried out to examine the distribution of six periodontopathic bacteria in deep periodontal pockets and to reconfirm the effect of Periocline
® on these periodontopathic bacteria. Samples from sixty-two periodontal pockets were collected at pocket depths of over 4 mm in twenty-one periodontitis patients aged 43 to 75 years. After sampling, Periocline
® was applied topically to the selected pockets once a week for four weeks and reexamined.
The detected rates of the periodontopathic bacteria were
Capnocytophaga sputigena (37.1%),
Prevotella intermedia (22.6%),
Porphyromonas gingivalis (22.6%),
Fusobacterium nucleatum (20.1%),
Actinobacillus actinomycetemcomitans (9.7%) and
Eikenella corrodens (4.8%) .
The distribution of the bacteria was compound because two or three bacterial species were found to coexist.
In view of the MIC of minocycline-HCI for these bacteria, increase of most of the measured bacteria was suppressed by the concentration of drugs, including Periocline
®. However, clinical strains of
P. i. were considered to have low susceptibility to minocycline-HCl.
In view of the effect of topical application of drugs, no significant differences were found.
From these results, it was suggested that Periocline
® contained effective concentration of minocycline-HCl.
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