Remarkable studies on O substance have been reported by many workers : Hooker and Anderson (1921), Witebsky (1927), Yamasaki (1933), Fukao (1937), Schiff (1927), and Miyazaki (1930). Eisler (1930) confirmed anti dysentery bacilli goat serum agglutinated human blood cells, and Kagaya (1937) found that in red blood cells of white rat, rabbit, pig, dog, sheep, and cat and in kidney of guinea pig and horse, there was a common antigen to human O type blood cells-the third heterogen antigen. Inoue (1943) devided O substance into three parts, i.e. O
I, O
II and O
III, by agglutinin reaction with sera, of sheep and domestic fowl immunized with human O type blood cells, dysentery bacilli, blood cells of rabbit and white rat, and kidney of guinea pig. This finding was confirmed later by Yamaguchi (1943) and Nakano (1951). Ôta and Oka (1952) and Sakimura (1952) studied on A and B substances in human saliva, and Watanabe (1952) on secretive condition of partial antigens of O substance in human saliva by precipitin reaction and classified human O type saliva not only in usual secretive and nonsecretive types but in the more detailed types. The present investigation was designated to make clear the secretive condition of partial antigens of O substance, by analysing the partial antigens of human O type saliva using the partial antibodies prepared by absorption.
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