The Kurume Medical Journal
Online ISSN : 1881-2090
Print ISSN : 0023-5679
ISSN-L : 0023-5679
10 巻, 2 号
選択された号の論文の7件中1~7を表示しています
  • KYOZO KOKETSU, RYOZO KITAMURA, HIROYUKI SOEDA
    1963 年 10 巻 2 号 p. 61-67
    発行日: 1963/04/20
    公開日: 2009/08/11
    ジャーナル フリー
    The membrane of frog's skeletal muscle fibers is known to be depolarized under the effect of 2, 4-dinitrophenol (DNP) in the bathing Ringer's solution. Since DNP is a powerful inhibitor of oxidative phosporylation of frog's skeletal muscle1), it is considered useful in investigating whether or not the maintenance of the bioelectrical potential is actually linked to energetic metabolism in excitable cells. The present paper is an account of experiments dealing with secondary restoration of the membrane potential of muscle fibers previously dropped under the effect of DNP. Results of this study may have an important bearing on the problem of the mechanism underlying the maintenance of the bioelectrical potential.
  • KENICHI NODA
    1963 年 10 巻 2 号 p. 68-75
    発行日: 1963/04/20
    公開日: 2009/08/11
    ジャーナル フリー
    It has been attempted to dynamically clarify the membrane property by comparatively studying the asymmetrical phenomena taking place in the membrane of frog sartorius muscle fibers. The change in transmembrane flux, depending on the direction of membrane potential changes, was also investigated. If the same resting potential shows the existence of a constant electrical driving force, the K efflux should be equal at the same level of resting potential. But this is not always true. Moreover, in Ringer solution it is very difficult for the sites of Ca ions in the membrane to be replaced by other cations.
  • MASAHISA SHINGU, SHIGERU YAMAMOTO, YOH NAKAGAWA
    1963 年 10 巻 2 号 p. 76-88
    発行日: 1963/04/20
    公開日: 2009/08/11
    ジャーナル フリー
    Within the past several yesrs, studies have been made of at least seven markers of poliovirus which can be determined by tissue culture, physical property and serological method, and which tend to discriminate between neurovirulent and attenuated strains. Strains possessing the d+ marker grow more rapidly than d- streains at low bicarbonate concentration under agar (1); strains possessing the MS+ marker grow more rapidly on the monkey kidney stable cell line than MS- strains (2); strains possessing the T+ marker grow readily at 40°C in contrast to the T- strains (3); CF antigen of strains possessing the CFt+ marker are more resistent than CFt-strains at various temperatures (4); strains possessing the A+ marker grow more rapidly on the primary human amnion cell than A- strain (5); strains possessing the E+ marker elute more rapidly from an DEAE column than E- strain (6). The most promising advance in the identification of virus strains is the intratypic sero-differentiation test investigated by McBride, Gard and Wecker (7), (8), (9). At the present time, the ECHO viruses consist of 28 serotypes. All 28 serotypes have been derived from the alimentary tract (and sometimes from the throat) of human beings. Many have been associated with outbreaks of benign aseptic meningitis (e, g., ECHO types 1, 2, 3, 4, 5, 6, 7, 8, 9, 12, 13, 14, 16, 19, 21, 24, ), and paralysis (e.g., ECHO types 2, 4, 6, 9, 11, 16). Although considerable attention should be paid to the studies on the properties of ECHO viruses, there is as yet very little information as to the general properties of the viruses in the tissue culture. The problem of the in vitro marker of ECHO viruses, that is to say, whether the in vitro marker test can be used in the ECHO viruses employing same method as for polioviruses, has been an interesting problem in the field of virulence of enteroviruses. The present paper deals with the multiplication of viruses in tissue culture and the in vitro marker test of ECHO viruses.
  • I. PHAGOCYTOSIS OF STREPTOCOCCUS BY VARIOUS KINDS OF ESTABLISHED CELLS
    HIROYUKI SHIKATA
    1963 年 10 巻 2 号 p. 89-99
    発行日: 1963/04/20
    公開日: 2009/08/11
    ジャーナル フリー
    Since Shepard's work (1) in which the HeLa cells, which were derived from human cervical cancer, had been for the studies on the mycobacteria-cell infection system, the application of the cells has been seen in a great number of reports concerning the host-parasite relationship (2-12). Because the established cells are not difficult to maintain in vitro, and these cells can be obtained easily any time under the same conditions, it may be expected that these established cells can be used for the studies on the host-parasite relationship in order to analyze the factors included in this infection system. In this paper, the fundamental data concerning the utilization of the established cells, especially the phagocytosis by varions kinds of cells, are described as a part of a series of the studies on the Streptococcus-cell infection system.
  • II. COMPARISON OF PHAGOCYTOSIS OF BACTERIA BY HeLa CELLS AND L CELLS
    HIROYUKI SHIKATA
    1963 年 10 巻 2 号 p. 100-104
    発行日: 1963/04/20
    公開日: 2009/08/11
    ジャーナル フリー
    Shepard (1-5) has been studying host-parasite relationships using HeLa cells. Similarly, the author cf this paper (6-8) has been reporting the studies on the hostparasite relationships between established cells and bacteria. In comparable researches on the phagocytosis rates of Streptococcus hemolyticus by various kinds of established cells, it was demonstrated that their phagocytosis rates varied according to the kind of cells used. Therefore, this present paper is to describe the comparisons between the phagocytosis of various kinds of bacteria by HeLa cells and by L cells.
  • SYOSAKU YASUTAKE, MASAHIRO MURAKAMI, TOMOHISA TANIZAKI
    1963 年 10 巻 2 号 p. 105-111
    発行日: 1963/04/20
    公開日: 2009/08/11
    ジャーナル フリー
  • PART I CHANGE OF ACIDIC POLYSACCHARIDES IN EXPERIMENTAL LIVER CIRRHOSIS OF RAT
    YUKIO TANAKA
    1963 年 10 巻 2 号 p. 112-128
    発行日: 1963/04/20
    公開日: 2009/08/11
    ジャーナル フリー
    A few biochemical investigations on liver cirrhosis have been reported by several investigators. They studied and discussed the problem mainly from a standpoint of chemical change in collagen protein included in the connective tissue of the cirrhosis.1)2)3)4)5)6)7) On the other hand, no suitable method for determining mucopolysaccharide, an important component of the ground substance of the connective tissue, has ever been established and many questions about mucopolysaccharide remain unsolved.It is now accepted that CSA plays an important role in the formation of connective tissue8)9)10). In the connective tissue formed while a wound is healing11) there appears first metachromasia -as well as PAS- positive substance and then a gradual growth of collagen. Though details of collagen growth in the liver are still unknown, it is possible that hepatic collagen may be produced in a different way from that of another connective tissue1)2)12). To follow up the role AMPS plays when liver cirrhosis occurs or improves is, therefore, of importance. Balasubrahmanyan12) who studied histo-chemically the fate of AMPS appearing in the tissue of liver cirrhosis, pointed out that histochemical observation alone was insufficient and thus purely chemical determination would be necessary in this field of investigation.A method for studying AMPS by measuring its hexosamine content5)6)7) is now widely adopted for the quantitative determination of AMPS, but this method is not the one most suitable since the liver is the only place carrying out the synthesis of glycoprotein13) and glycoprotein molecules contain a great amount of hexosamine14)15). Therefore it can not be expected that there is a proportional ratio inamount between hexosamine and AMPS, and it also is unknown how each fraction of AMPS (HA, CSA, Hep, etc.) correlates with the connective tissue formation.Thus, the author undertook to fractionate AMPS into HA, CSA and Hep by a modified method of Scott's16)17) to examine separately the role of each fraction played in the formation of hepatic connective tissue when the experimental liver cirrhosis was introduced by using hexuronic acid in place of hexosamine as an index of AMPS.
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