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Volume 18, Issue 4
Displaying 1-8 of 8 articles from this issue
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II. DENSITY GRADIENT SEDIMENTATION OF THE ANTIGENIC COMPONENTSEIJI KIMOTO, TAKAYO KURANARI, MITSUWO HARA1971 Volume 18 Issue 4 Pages 169-175
Published: December 15, 1971
Released on J-STAGE: August 11, 2009
JOURNAL FREE ACCESSProteinpolysaccharide complexes of human costal cartilage were extracted by the so-called “dissociative procedure” to ensue the integrity of their macromolecular structure. After centrifugation in cesium chloride density gradient, the macromolecular complex sedimented to the tube bottom as a dense component. Its hya luronidase-digested one gave a specific precipitate line against antiserum on an Ouchterlony assay. The antigenic moiety, possessing a chemical nature of sialoprotein rather than core protein of proteoglycan, existed as an aggregated complex of macromolecular protein-polysaccharide.View full abstractDownload PDF (3892K) -
TERUO MIYAGAWA, HIDEMASA KAJI, YOSHIO SAKATA, SHIGEYOSHI MINAKAWA, RYO ...1971 Volume 18 Issue 4 Pages 177-181
Published: December 15, 1971
Released on J-STAGE: August 11, 2009
JOURNAL FREE ACCESSThe use of paper chromatography has been demonstrated to be a simple and efficient means for the detection and separation of glutathione from the constituent amino acids and the related metabolite. The use of Nethylmalemide to stabilize the sulfhydryl group during chromatography was found to be a marked advance. This technic was applied to the study of glutathione in the epidermis. The incorporation of 14C-glycine into glutathione was demonstrated under the homogenate of rat epidermis.View full abstractDownload PDF (549K) -
K. NODA1971 Volume 18 Issue 4 Pages 183-193
Published: December 15, 1971
Released on J-STAGE: August 11, 2009
JOURNAL FREE ACCESS1) Evidence obtained from washout experiments of 45Ca ions in frog sartorius muscles has led to the conclusion that IAA promotes Ca++ ion release from the muscle fibre surface. The detailed mechanism to produce the enhancement has been investigated from the viewpoint of a direct effect of IAA on membrane lipoprotein, especially on its SH groups.2) As the concentration of externally applied IAA was increased from a threshold concentration of about 0.1 mM, continuous increase in 45Ca efflux began to appear. Near 2 mM, the increase in 45Ca efflux was complicated by an addition of the reduction in it.3) The increase in 45Ca dissociation was presumed as a result of the effect of IAA on membrane SH groups, while the reduction in the same ion movement was supposed as failed glycolysis. Accordingly, 45Ca efflux increase by IAA was not a response to the drug but a sequence of the destruction of normal lipoprotein conformation. The experiments of 22Na efflux also proved that the increase in 22Na efflux due to IAA was a consequence of disorganization of chemical membrane architecture.4) 45Ca efflux increase by IAA was not short-lived but continuous. IAA prevented the appearance of immobilizing effect of trypsin on membrane 45Ca ions. DNFB demonstrated a marked transient increase in 45Ca dissociation. As far as the kinetic behaviors of membrane 45Ca ions are concerned, the sites of Ca++ ions relating to IAA are clearly different from the sites of Ca++ ions connecting to trypsinization or DNFB action.5) 45Ca ions mobilized by IAA are not Ca++ ions extremely susceptible to the external Ca++ ions, which directly govern the concentration of Ca++ ions in the superficial layer of the membrane. When Sr++ ions were used instead of Ca++ ions in Ringer, 45Ca efflux was not stimulated by IAA, suggesting that the membrane structure maintaining properly by Ca++ ions may be a premise for an enhanced Ca++ efflux increase by IAA . The immobilizing effect of no external K+ on 45Ca efflux was hardly seen in the presence of IAA.6) The structural change of methylative amino acids, playing important role in performing biosynthesis or maintenance of membrane phospholipid, especially lecithin, produced by IAA, probably results indirectly in labilization of membrane Ca++ ions through rearrangement of the binding pattern altered secondary to conformational change in membrane lipoprotein.View full abstractDownload PDF (1414K) -
KOYO OKABE, SATOSHI SHIRAISHI1971 Volume 18 Issue 4 Pages 195-199
Published: December 15, 1971
Released on J-STAGE: August 11, 2009
JOURNAL FREE ACCESSInfection experiments of Oncomelania nosophora with Rhabditis oncomelaniae were carried out both in the laboratry and the field. In the laboratry experiments, 20, 40, 80, 330, and 100 Rhabditis were infected per one snail. The infection rate of the snails with the nematodes was very high (80 to 100%), when the small sized petri dishes with filter papers and tap water were used. But the mortality of the snail was very low. In the field experiments, 15, 000 and 450, 000 nematodes were sprayed with one liter of water by the use of watering can in one square meter of the habitat of Oncomelania snails. Surveys were carried out 7, 15, and 21 days after spraying.The infection rate of the snails with this kind of Rhabditis was not so high, and decreased with time. But the mortality increased with time, though this rate was not high. The authors think that it may be possible to get desirable result by improvement of the spraying method.View full abstractDownload PDF (625K) -
KOYO OKABE, MINORU AKUSAWA, TAMIKO HANAMURA1971 Volume 18 Issue 4 Pages 201-204
Published: December 15, 1971
Released on J-STAGE: August 11, 2009
JOURNAL FREE ACCESSThe existence of Schistosoma japonicum antigen was ascertained in feces of rabbits infected with S. japonicum by means of precipitin reaction between anti-S. japonicum rabbit serum. In some example, no positive reaction was found. This may be depend on the quantitative relation of antigen and antibody in such examples.View full abstractDownload PDF (1761K) -
A STUDY OF 89 CASES AMONG 3890 NECROPSIES AND A STUDY OF ALPHA-FETOPROTEIN IN THE SERUM OF 17 CASES.MASAMICHI KOJIRO1971 Volume 18 Issue 4 Pages 205-222
Published: December 15, 1971
Released on J-STAGE: August 11, 2009
JOURNAL FREE ACCESS1. Eighty-nine primary liver cancers were noted among 3890 necropsies performed at Kurume University School of Medicine, in the period 1929-1971, a frequency of 2.28 per cent.2. The sex ratio of male to female was thus 7 : 1. The average age was 57.26±11.82 years. The average age for male was 56.96±11.'77 years and for female 59.60±10.76 years. Agewise, the largest frequency was in the sixth decade in life.3. General malaise was the most common as an initial symptom. Abdominal pain, ascites, enlargement of the liver, icterus, and fullsensation of the abdomen were prominent symptoms in the course of primary liver cancer. The average survival time was very short compared with other malignant tumors. About fifty per cent of patients died within three months after the appearance of initial symptoms.4. Bleeding from digestive organs including esophageal varices and tumor, and hepetic coma were frequent as a cause of death.5. Ascites was seen in 86 per cent and icterus was seen in 67.3 per cent of all cases. Association of liver cirrhosis somewhat effected liver and spleen weight.6. Metastasis was relatively rare compared with other malignant tumors, a frequency of 59.6 per cent. The lungs were the most frequent recipients (42.7%).7. The gross classification of primary liver cancer was carried out as follows; nodular form, massive form, and diffuse form.8. Histologically, the fifty-seven liver cell carcinomas were classified into trabecular, glandular, mixed type of trabecular and glandular; giant cell carcinoma, and small cell carcinoma. Seven cases of bile duct carcinoma and one case of combined carcinoma were observed.9. Most frequent associted lesion was liver cirrhosis and most of associated liver cirrhosis were Miyake's B type. Gastroduodenal ulcer and gastric erosion were observed in 30.3 per cent of all cases. Thirteen of the 89 primary liver cancers were associated with schistosomiasis japonica, but no causal relationship could be histologically found.10. Detection of alpha-f etoprotein was carried out in recent seventeen cases. Alpha-fetoprotein was positive in eleven cases but negative in six cases. No marked relationship between alpha-fetoprotein and histological findings could be found.View full abstractDownload PDF (7951K) -
HIDEFUMI KABUTA, SHIGERU YAMAMOTO, YOH NAKAGAWA1971 Volume 18 Issue 4 Pages 223-229
Published: December 15, 1971
Released on J-STAGE: August 11, 2009
JOURNAL FREE ACCESSNeutralization kinetic analysis for appropriate seven strains of herpes simplex virus (HSV) was performed using guinea pig antisera. Six strains among those were different in the diagnosis or plaque sizes in African grivet monkey kidney (GMK) cells. In the first place, the reliability of neutralization rate constant (K values) was examined. K values of each antiserum against every virus strain were determined under the suitable condition and the normalized neutralization constants (NK values) were calculated for antigenic comparison. The average NK value against the standard HF strain of six heterologous antisera was 41, which was the lowest of those against the virus strains tested. On the other hand, Z strain was neutralized most rapidly by heterologous antisera. The average NK value was 94 which was the highest value. For the serological differentiation by NK value, anti-HF serum was unsuitable. On the contrary, NK values of anti-Z serum were the lowest and the most separate values against the heterologous strains, so that it will be the most excellent for serological differentiation. The results were not so successively feasible as might have been expected. The some reasons were discussed.View full abstractDownload PDF (858K) -
HIROMICHI YANO, HIROYOSHI MIZOTE1971 Volume 18 Issue 4 Pages 231-232
Published: December 15, 1971
Released on J-STAGE: August 11, 2009
JOURNAL FREE ACCESSDownload PDF (2162K)
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