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Volume 34, Issue 2
Displaying 1-7 of 7 articles from this issue
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ATSUMI WAKABAYASHI, MITCHEL MITSUO YOKOYAMA1987 Volume 34 Issue 2 Pages 49-52
Published: August 01, 1987
Released on J-STAGE: August 11, 2009
JOURNAL FREE ACCESSAn automated instrument was constructed and used for the DNA spot test. This assay gave more quantitative information than the conventional test on the presence of antibodies to DNA in the test serum. The results were reproducible and also the procedure was simple.View full abstractDownload PDF (425K) -
RYOHEI OGURA, TOSHIHIKO SAKANASHI, OSAMU NAGATA, MASAYASU SUGIYAMA, KI ...1987 Volume 34 Issue 2 Pages 53-58
Published: August 01, 1987
Released on J-STAGE: August 11, 2009
JOURNAL FREE ACCESSThe thiobarbituric acid (TBA) reaction method established by Yagi to determine the lipid peroxide level in serum and animal tissues was applied to isolated mitochondria with some modifications. The TBA reaction was carried out in acetic acid (pH 2.0) in the presence of 0.7 % sodium dodecyl sulfate at 95°C for 45 min. Chloroform was used to remove the lipid and protein from the reaction mixture. An aqueous layer was used for spectrophotometric measurement at 532.5 nm to determine the TBA reaction product with lipid peroxide. To evaluate the TBA assay system, the lipid peroxide content of mitochondrial suspensions exposed to ultraviolet light was examined. The lipid peroxide formed in mitochondria was proportional to the dose of ultraviolet energy. The lipid peroxide of methyl linoleate exposed to ultraviolet light was determined both with the TBA reaction and with the hydroperoxide reaction. The linear increase in TBA values following UV exposure was similar to the increase of hydroperoxide values determined iodometrically. The TBA assay was shown to be a good predictor of lipid peroxide formed in mitochondria. This assay system is useful for lipid peroxide determination in various tissues: epidermis, Ehrlich ascites tumor cells and B-16 melanoma culture cells.View full abstractDownload PDF (637K) -
KIMINORI KAJIYAMA, MASAYASU SUGIYAMA, HIROSHI IWAHASHI, TOSHIHIRO HIDA ...1987 Volume 34 Issue 2 Pages 59-64
Published: August 01, 1987
Released on J-STAGE: August 11, 2009
JOURNAL FREE ACCESSAdriamycin (ADR), which is a potent antitumor agent, has severe cardiotoxicity; however, the underlying mechanism is not well understood. In our present study, the electron spin resonance (ESR) technique was employed to analyze the physical properties of cardiac mitochondrial membrane treated with ADR. After intercalcation of spin probe into the isolated cardiac mitochondrial membrane, mitochondria were incubated with 3.45×10-4M ADR for 10 minutes at 37°C, the membrane fluidity was measured by ESR spin probe; 5-doxyl stearic acid (5-DSA) and. 12-doxyl stearic acid (12-DSA). The ADR in vitro treatment resulted in an elevation of the order parameter S and S (T_??_). It was suggested that the membrane fluidity of cardiac mitochondria decreased in both 5-DSA and 12-DSA regions of the lipid bilayer.View full abstractDownload PDF (655K) -
TOYOHIKO URAKAWA, NOBUYUKI HAMADA, MASAHISA SHINGU1987 Volume 34 Issue 2 Pages 65-73
Published: August 01, 1987
Released on J-STAGE: August 11, 2009
JOURNAL FREE ACCESSDense particles (buoyant density in CsCl: 1.45-1.47 g/cm3) were isolated from HeLa cells infected with swine enteroviruses and coxsackievirus type B5. These dense particles had important properties which were different from those of poliovirus and other picornaviruses described previously; for instance, they could be isolated under various conditions of virus growth. The smallest capsid polypeptide, VP4, was not detected by SDS-PAGE analysis. The preparations of dense particles were non-infectious and showed H antigenicity by a modified enzyme immunoassay. Judging from these properties, dense particles might be equivalent to “A particles”. These findings suggest that the VP4 polypeptide is responsible for the conformational stabilization of the intact virion.View full abstractDownload PDF (2302K) -
TERUO HIEDA, TAMAKA OKINA, HIDETOSHI WAKAE, SHIGERU HASHIMOTO, FUMIO G ...1987 Volume 34 Issue 2 Pages 75-81
Published: August 01, 1987
Released on J-STAGE: February 08, 2010
JOURNAL FREE ACCESSA case of neurilemoma on the palatal region of a 44 year-old man was observed. The patient noticed a tumor on the right palatal region during treatment for dental caries, and came to our hospital for an examination. No particular change in facial findings was observed, and a small tumor (about 15×12 mm) covered with healthy mucosa was found in the right palatal region. The clinical diagnosis was pleomorphic adenoma, and the patient was operated on under general anesthesia. Histopathologically, the tumor tissue was full of hyperplastic nuclear spindle cells partially in a shelf arrangement. S-100 protein staining by the PAP method gave a positive reaction. With scanning and transmission electron microscopy the tumor tissue appeared fascicular and had a convoluted arrangement in each direction. These findings were suggestive of a Schwann cell origin for the tumor.View full abstractDownload PDF (7630K) -
HISAO IKEDA, OSAMU YOSHIGA, KEIGO SHIBAO, TAKAFUMI UENO, YOSHIO HIDAKA ...1987 Volume 34 Issue 2 Pages 83-89
Published: August 01, 1987
Released on J-STAGE: August 11, 2009
JOURNAL FREE ACCESSRight ventricular ejection fractions were determined by videodensitometric computer analysis of digital subtraction angiography in 50 patients with various cardiac diseases. Using intravenous injection of 35 ml sodium meglumin diatrizoate at a constant rate of 12 ml/min, serial right ventriculograms were obtained in the right anterior oblique projection. Right ventricular enddiastolic and end-systolic counts and ejection fractions were obtained by integrating densitometric counts within the, right ventricular region of interest, in addition to application of the conventional pyramidal method. The obtained right ventricular densitometric counts correlated closely (r=0.91) with the single-plane right ventricular volumes. The right ventricular ejection fraction determined by video-densitometry also exhibited satisfactory correlation (r=0.74) with that by single-plane ventriculography. Thus, videodensitometric analysis of digital subtraction right ventriculograms are an acceptable method for the determination of the right ventricular ejection fraction, and therefore videodensitometric analysis is recommended as a convenient method for clinical practice.View full abstractDownload PDF (1947K) -
The Diagnostic Role of EchocardiographyTEIJI AKAGI, ON TOYODA, NORIKO RIKITAKE, TAKATOSI MIKASIMA, OSAMU INOU ...1987 Volume 34 Issue 2 Pages 91-95
Published: August 01, 1987
Released on J-STAGE: August 11, 2009
JOURNAL FREE ACCESSA cardiac tumor was detected in a 10 day old boy using 2-dimensional echocardiography. A heart murmur had indicated the presence of congenital heart disease; however a tumor in the right ventricular outflow tract was eventually detected by 2-dimensional echocardiography. Pulsed Doppler echocardiography was used and the pressure gradient across the tumor was determined to be 18 mmHg. The stenosis caused by the tumor had developed progressively, and a resection of the right ventricular tumor was performed at an age of 7 months. The results of the histological analysis indicated the tumor was a rhabdomyoma. Since a patient with a cardiac tumor generally has no specific symptoms, two-dimensional echocardiography was the most useful and most rapid noninvasive method to determine the extent and the location of the tumor. Furthermore the degree of stenosis produced by the tumor could be evaluated using pulsed Doppler echocardiography.View full abstractDownload PDF (4545K)
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