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Volume 35, Issue 4
Displaying 1-10 of 10 articles from this issue
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TOSHIHIKO SAKANASHI, MASAYASU SUGIYAMA, TOMOMASA SUEMATSU, TETSURO NAK ...1988 Volume 35 Issue 4 Pages 159-169
Published: March 31, 1989
Released on J-STAGE: February 08, 2010
JOURNAL FREE ACCESSThe response of eukaryocytes such as cultured B-16 melanoma cells to oxidative stress has been examined following ultraviolet (UV) exposure. Changes of lipid peroxide content, membrane lipid composition and cell membrane fluidity were measured. Lipid peroxide content, expressed as malondial-dehyde content, increased immediately after UV exposure and then rapidly decreased to less than the non-exposed controls, 6 hrs after UV exposure. The polyunsaturated fatty acid (PUFA) content, especially the content of arach-idonic acids (AA), of the phospholipids of the cells increased and the chole-sterol/phospholipid molar ratio decreased, 6 hrs after UV exposure. No further change was observed in phospholipid class distribution of the cells. These re-sults indicate that eukaryocytes, such as B-16 melanoma cells, have the ability to repair or remodel damaged membranes, partly through replacement of oxidized fatty acids in membrane phospholipids via deacylation and reacylation. On the other hand, the cell membrane fluidity, measured by spin label techniques, decreased immediately after UV exposure and then increased 6 hrs after UV exposure. It can be concluded that the biphasic change of cell membrane fluidity reflects the repair or remodeling of the membrane damaged by oxidation.View full abstractDownload PDF (1382K) -
Tissue Culture Cells, Cardiac Mitochondria, Erythrocytes and Epidermal CellsRYOHEI OGURA, MASAYASU SUGIYAMA, TOSHIHIKO SAKANASHI, TSUYOSHI NINOMIY ...1988 Volume 35 Issue 4 Pages 171-182
Published: March 31, 1989
Released on J-STAGE: February 08, 2010
JOURNAL FREE ACCESSThe spin-labeling technique with electron spin resonance (ESR) was introduced to determine membrane fluidity in plasma membranes. Three kinds of doxylstearic acid were used as spin labeling agents. The experimental conditions of ESR measurement, such as spin probe concentration, number of cells, decay of ESR specturum, incubation time, ESR cavity temperature, and microwave power, were discussed with the aim of obtaining reproducible ESR spectra. As biological material, B-16 melanoma cells, Ehrlich ascites tumor cells, epidermal cells, erythrocytes and cardiac mitochondria were used.View full abstractDownload PDF (3990K) -
SATOSHI MIHARA, KOZO IKEDA, SYOGORO NISHI1988 Volume 35 Issue 4 Pages 183-192
Published: March 31, 1989
Released on J-STAGE: February 08, 2010
JOURNAL FREE ACCESSIntracellular recordings were made from neurons of the cardiac ganglion of the guinea-pig heart. Application of muscarinic agonists produced a depolarization in the majority of neurons. The change in membrane conductance underlying the depolarization consisted of an initial low-conductance phase followed by a high-conductance phase. The first component was probably due to suppression of a potassium conductance, and the second was likely generated by activation of a chloride conductance. Both components of the depolarization were blocked by pirenzepine with an antagonist dissociation constants (KD) of 200-900 nM, suggesting that both are mediated through a muscarinic M2 receptor.View full abstractDownload PDF (7168K) -
A Possible Relationship to Ocular SarcoidosisTOMOKO KUBOSHIRO, HISAHARU YOSHIOKA1988 Volume 35 Issue 4 Pages 193-199
Published: March 31, 1989
Released on J-STAGE: February 08, 2010
JOURNAL FREE ACCESSThis report concerns four patients who exhibited characteristic ocular signs of Birdshot retinochoroidopathy and were diagnosed as suffering from sarcoidosis on the basis of histological evaluation of lymph node biopsy. The findings in the present series suggest that sarcoidosis may be one of the cause of birdshot retinochoroidopathy. Two patients exhibited reddish appear-ance of the posterior fundus (“sunset-glow” fundus). This report suggests when “sunset-glow” fundus is seen in the patient with uveitis, the presence of sarcoidosis should be considered besides that of Harada's disease and sympathetic ophthalmia.View full abstractDownload PDF (2718K) -
TAKASHI NISHIDA, TOSHIO OBUCHI, TOSHI SASAKI, NAOKI NAGASUE, MICHIAKI ...1988 Volume 35 Issue 4 Pages 201-205
Published: March 31, 1989
Released on J-STAGE: February 08, 2010
JOURNAL FREE ACCESSA case of an extragenital malignant mixed Müllerian tumor with heterologous elements is presented. The tumor originated from the pelvic peritoneum, as confirmed with a laparotomy and at autopsy. Combination chemotherapy with ifosfamide, adriamycin, cisplatin and etoposide resulted in a clinical remission, but the duration of the remission was limited. Histogenesis and therapeutic intractability are briefly discussed.View full abstractDownload PDF (5559K) -
OSAMU MORI, HIROSHI HACHISUKA, HIROFUMI NOMURA, KEIJI OKUBO, YOICHIRO ...1988 Volume 35 Issue 4 Pages 207-209
Published: March 31, 1989
Released on J-STAGE: February 08, 2010
JOURNAL FREE ACCESSThe effect of etretinate, applied systemically, on epidermal Langerhans cells was studied. The numbers and shapes of Langerhans cells were observed on split epidermis, and the percentage of Langerhans cells in whole epidermal cells was determined with a flow cytometrial system. On an epidermal sheet, the number of Langerhans cells changed biphasically, first the number increased, then decreased, and finally it returned to normal. The percentage of Langerhans cells was drastically decreased by etretinate and the low value was maintained during the etretinate treatment. This difference may be due to a decrease in the cell surface marker.View full abstractDownload PDF (403K) -
KATSUHIRO MIZOGUCHI, HIROTO JOJIMA, MASAMITSU TANAKA, MARIKO HOTTA, HI ...1988 Volume 35 Issue 4 Pages 211-215
Published: March 31, 1989
Released on J-STAGE: February 08, 2010
JOURNAL FREE ACCESSThe sphenoid sinus is deeply situated in the nasal cavity and infection of this sinus is less common than infections of other sinuses. Accordingly, sphenoid sinus infections are frequently misdiagnosed on initial evaluation. The walls of this sinus are adjacent to the pituitary gland, optic nerve and cavernous sinuses. A case of sphenoid sinusitis associated with meningitis, visual disturbances and total ophthalmoplegia was reported. The patient, a 58 year-old female, complained of visual disturbances and deep pain in both retroorbital regions. On admission, the neurological examination revealed a bilateral choked disc with III rd, IV th, V th and VI th cranial nerve palsies. The cerebrospinal fluid was clear with a pressure of 280 mmH2O, and contained 93 cells per mm3., consisting mainly of lymphocytes. The sugar content was 55 mg /dl and the protein content was 344 mg/dl. CT and MRI showed that the sphenoid sinus was filled with pus, but there was no evidence of bone destruction. Surgical drainage was performed for the sphenoid sinusitis. The CSF findings returned to the normal ranges, but the visual disturbance and total ophthalmoplegia still remained, two months after the drainage. The shenoid sinusitis can easily spread to the pituitary gland, optic nerve and cavernous sinuses, and result in serious irreversible neurologic complications. This case demonstrates that early diagnosis and treatment are important.View full abstractDownload PDF (3956K) -
TAKASHI AKASU, HIROSHI HASUO1988 Volume 35 Issue 4 Pages 217-220
Published: March 31, 1989
Released on J-STAGE: February 08, 2010
JOURNAL FREE ACCESSDownload PDF (502K) -
TOSHIHIKO NISHIMURA, TAKASHI AKASU1988 Volume 35 Issue 4 Pages 221-224
Published: March 31, 1989
Released on J-STAGE: February 08, 2010
JOURNAL FREE ACCESSDownload PDF (425K) -
MITSUNARI HIGUCHI, OSAMU MORI, HIROSHI HACHISUKA1988 Volume 35 Issue 4 Pages 225-227
Published: March 31, 1989
Released on J-STAGE: February 08, 2010
JOURNAL FREE ACCESSDownload PDF (3756K)
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