The Kurume Medical Journal
Online ISSN : 1881-2090
Print ISSN : 0023-5679
ISSN-L : 0023-5679
Volume 44, Issue 2
Displaying 1-11 of 11 articles from this issue
  • MASAO HAMASAKI, KENTARO SAWADA, HITOSHI MATSUO, TOSHINORI MAEHARA, HID ...
    1997 Volume 44 Issue 2 Pages 71-81
    Published: June 14, 1997
    Released on J-STAGE: August 11, 2009
    JOURNAL FREE ACCESS
    The morphological properties of cell organelles and nuclei in rat hepatocytes were compared, when the hepatocytes were either chemically fixed in glutaraldehyde and osmium (GA-Os), chemically fixed only in osmium (Os-2h) or physically fixed with a microwave-irradiated osmium (Mw-Os) fixative for a short duration. Judging from the fine structure, the mitochondria and rER were best preserved after physical fixation; because of the smooth surfaces and regular open contours, but both organelles shrank in the two chemical fixatives. The Golgi complexes and peroxisomes of the hepatocytes were not markedly changed by any of the fixative procedures. However the lysosomes and sER were changed to some extent after physical fixation. In the nucleus, the two kinds of chromatins and the nucleolus were best preserved with physical fixation. In conclusion, physical fixation is a very simple and fast procedure that is convenient for routine electron microscopy if some attention is paid to the morphological changes.
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  • SYOJI NISHIOKA
    1997 Volume 44 Issue 2 Pages 83-90
    Published: June 14, 1997
    Released on J-STAGE: August 11, 2009
    JOURNAL FREE ACCESS
    Although lichen sclerosus et atrophicus at an early stage and morphea can be differentiated clinically and histologically, both diseases at a late stage present diagnostic difficulties. In this study, collagen and acid glycosaminoglycans in the skin of both morphea and lichen sclerosus et atrophic us were examined by polarization microscopy. Picrosirius red and Toluidine blue (pH 5.8) were used as stains. Under polarized light, the picrosirius red-stained collagen fibers appeared green in the papillary and reticular dermis of morphea, except the fibers immediately below the epidermis, which appeared orange yellow. In lichen sclerosus et atrophicus, the collagen fibers appeared green in the reticular dermis at both early and late stage. In the papillary dermis the fibers appeared orange at an early stage and greenish orange at a late stage. Toluidine blue-stained birefringence in morphea diminished in the presence of MgCl2 at 0.2 M, in lichen sclerosus et atrophicus in the presence of MgCl2 at 0.3 M. Histologically, there were some differences in epidermal changes between the two diseases; the epidermis was thick in morphea and thin in lichen sclerosus et atrophicus. This difference seems to relate to that in the fibers beneath the epidermis.
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  • KIKUO KOUFUJI, JINRYO TAKEDA, ATSUSHI TOYONAGA, ISSEI KODAMA, KEISHIRO ...
    1997 Volume 44 Issue 2 Pages 91-97
    Published: June 14, 1997
    Released on J-STAGE: August 11, 2009
    JOURNAL FREE ACCESS
    Seventy-seven octogenarian patients with gastric cancer (Group 1) were retrospectively investigated and compared with patients of age from 75 to 79 years (Group 2). The incidence rates for renal impairment, hepatic impairment, and hypoproteinemia, as preoperative complications, were higher in Group 1 than in Group 2 (each p<0.05). The resectability rate was 83% in Group 1 and 89% in Group2. D1 lymphadenectomy was performed for 45% of Group 1 and for 18% of Group 2 (p<0.001). On the other hand, D2 lymphadenectomy was performed for 39% of Group 1 and for 71% of Group 2 (p<0.001). A postoperative complication occurred in 25% of Group 1 and in 9% of Group 2 (p<0.01). However, the postoperative mortality rate was zero in both groups. The t1 (early cancer) rate was 27% in Group 1 and 41% in Group 2 (p<0.05). On the other hand, t2 was 31% in Group 1 and 19% in Group2 (p<0.05). The overall 5-year-survival rate was 38% in Group 1 and 48% in Group2. When causes of mortality other than gastric cancer were excluded, the 5-year-survival-rate was 51% in Group 1 and 67% in Group 2 (p<0.01).
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  • SOMSONG NANAKORN, BUALONG CHIN-ON, RYOKO OSAKA, HIROKI INUTSUKA, KATSU ...
    1997 Volume 44 Issue 2 Pages 99-104
    Published: June 14, 1997
    Released on J-STAGE: August 11, 2009
    JOURNAL FREE ACCESS
    This report describes physical, psychological and overall health complaints of 513 female Thai nursing students obtained by using the Cornell Medical Index questionnaire (C.M.I.) Thai version. The subjects were 17 to 24 years old in their 1st, 2nd, 3rd, or 4th year-class of nursing school in the northeast region of Thailand. The Pearson correlation coefficient shows an adequate internal consistency of the C.M.I. The observed C.M.I. scores ranged from 1 to 110 with amean of 36.6. The highest percentages of yesresponse, among physical and psychological complaints, were observed in the questions concerning the genitourinary system, and sensitivity respectively. A statistically significant inverse correlation was observed between year of nursing school and C.M.I. scores in physical, psychological and overall health complaints: the mean C.M.I. score on physical and overall health complaints of the 1st and the 3rd year-class students were significantly higher than those of the 2nd and the 4th year-class.
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  • TATSURU HARA, HIROJI KANBARA, MASANORI NAKAO, TOSHIHIDE FUKUMA
    1997 Volume 44 Issue 2 Pages 105-113
    Published: June 14, 1997
    Released on J-STAGE: August 11, 2009
    JOURNAL FREE ACCESS
    Bloodstream forms of the parasitic protozoa Trypanosoma brucei gambiense derive all of needed energy through an unusual glycolysis. In an earlier study, we showed that D-mannose specifically inhibited the growth of bloodstream forms of T. b. gambiense. We investigated D-mannose transport into the T. b. gambiense bloodstream forms and its metabolism in the initial phase of the glycolytic pathway. D-Mannose was transported rapidly into the bloodstream forms of T. b. gambiense (Km=378μM), and Dglucose competitively inhibited D-mannose uptake. D-Mannose and D-glucose are transported into bloodstream trypanosomes by the same carrier. Hexokinase from the bloodstream trypanosomes could convert D-mannose to D-mannose 6-phosphate (Km=155.8μM; Vmax=0.93μmol/min/mg protein), with kinetic properties very similar to D-glucose phosphorylation (Km=199.4μM; Vmax=1.15μmol/min/mg protein). D-Mannose 6-phosphate could be further metabolized in the glycolytic pathway. However, the metabolic rate was extremely slow, and D-mannose 6-phosphate accumulated in the glycosomes. D-Mannose may cause growth inhibition of bloodstream trypanosomes through an extremely high concentration of D-mannose 6-phosphate in the glycosomes.
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  • YASUMITSU OKUBO
    1997 Volume 44 Issue 2 Pages 115-123
    Published: June 14, 1997
    Released on J-STAGE: August 11, 2009
    JOURNAL FREE ACCESS
    Several reports described a favorable effect of “low-dose and long-term” erythromycin (EM) on chronic obstructive pulmonary diseases including diffuse panbronchiolitis (DPB), although its mechanism still remains obscure. We investigated the effect of some macrolides, erythromycin (EM), rokitamycin (RKM), midecamycin (MDM) on the expression of neutrophil adhesion molecule Mac-1 using LPS-stimulated human whole blood as an experimental vivo model. Samples from six healthy volunteer were treated with various concentrations (0.02 ug/m1-20 ug/ml) of EM, RKM and MDM for 1 to 3 hs. Surface expression of Mac-1 antigen was determined by use of flow-cytometry. When pretreated with EM and MDM for 1 and 3 hs, significant reduction in Mac-1 expression was observed, but with RKM no substantial reduction. These findings indicate that some macrolides such as EM suppress the surface expression of Mac-1 on neutrophil and may alleviate local alveolar injury in chronic pulmonary diseases.
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  • MASASHI GOTO, HIROSHI HASUO, TAKASHI AKASU
    1997 Volume 44 Issue 2 Pages 125-133
    Published: June 14, 1997
    Released on J-STAGE: August 11, 2009
    JOURNAL FREE ACCESS
    Properties of ligand-gated K+ channels were examined in neurons of rat dorsolateral septal nucleus (DLSN). Application of muscarine (30μM), 5-hydroxytryptamine (5-HT, 10μM), adenosine (100μM), baclofen (10μM) and norepinephrine (NE, 10μM) to DLSN neurons caused hyperpolarizing responses associated with decreased membrane resistance. Hyperpolarizations induced by muscarine increased their amplitudes at membrane potential between -70 and -50 mV. Baclofen- and NE-induced hyperpolarizations were less sensitive to voltage. The agonist-induced hyperpolarizations decrease in amplitudes and reversed at a membrane potential between -90 and -100 mV. Ba2+ (1 mM) blocked all agonist-induced hyperpolarizations in DLSN neurons. Tetraethylammonium (TEA, 3 mM) blocked the muscarine-induced hyperpolarization but not the hyperpolarizations induced by the other agonists. Extracellular Cs+ and glibenclamide did not block the agonist-induced hyperpolarizations. These results suggest that muscarine, 5-HT, adenosine, baclofen and NE cause the hyperpolarization by increasing the activity of Ba2+-sensitive K+ channels, probably the GTP-binding protein (G-protein) activated inward rectifier K+ (GIRK) channels in DLSN neurons.
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  • MAMORU ARIYOSHI, KENSEI NAGATA, KIMIAKI SATO, MANABU KUBO, KOJI HIRAOK ...
    1997 Volume 44 Issue 2 Pages 135-139
    Published: June 14, 1997
    Released on J-STAGE: August 11, 2009
    JOURNAL FREE ACCESS
    We present five patients with acute traumatic hemarthrosis of the knee who also had hemorrhage within the bone marrow around the knee detected by magnetic resonance imaging. No additional bony, ligamentous, meniscal or osteochondral injuries were evident from clinical examination, initial and repeated plain radiographs, or magnetic resonance imaging. The main symptoms were sharply localized pain and tenderness of the contused area. When hemarthrosis was demonstrated at the first office visit, three cases had fat droplets in the blood. Two patients with hemarthrosis of the knee undergoing arthroscopy showed no obvious lesions of intra-articular structures. Instead they showed congestion of the joint capsule located in accordance with the traumatic episode. Magnetic resonance imaging demonstrated marrow abnormalities as diffusely low and high signal intensity areas in T1-weighted and T2-weighted images, respectively, in locations consistent with the traumatic episode and the symptoms. These disappeared within an average of 13 weeks (range in 4 cases, 12 to 16 weeks). These findings were considered diagnostic of bone contusion. Symptom duration was about 2 weeks in all cases. In conclusion the clinician should consider the possibility of coexisting bone contusion in cases of acute traumatic hemarthrosis of the knee with no evidence of bone or intra-articular lesions on clinical examination and conventional radiographs.
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  • Report of Three Cases and Review of Literature
    AKIHIRO HAYASHI, SHINZO TAKAMORI, KOHSUKE TAYAMA, MASAHIRO MITSUOKA, S ...
    1997 Volume 44 Issue 2 Pages 141-146
    Published: June 14, 1997
    Released on J-STAGE: August 11, 2009
    JOURNAL FREE ACCESS
    We report three recently resected cases of thymolipoma. Two cases (case 2 and 3) were female and the other (case 1) was male. The age of these three were 41 years, 63 years, and 29 years, respectively. While two cases (case 1 and 2) were asymptomatic, the other (case 3) had associated myasthenia gravis. Preoperative examination in each case was performed using chest CT scan or chest MRI, or both. Only one case (case 1) was diagnosed as thymolipoma preoperatively, and this was by a chest MRI showing the mass in continuity with the thymus. The diagnosis of each lesion was confirmed histologically by their composition of lobules of mature adipose tissue with minute islands of thymic tissue consisting of epithelial cells, lymphocytes, and Hassall's bodies.
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  • HISATO TANAKA, KOH-ICHI YAMAKI, TSUYOSHI SAGA, YOSHIAKI DOI, TETSUSHI ...
    1997 Volume 44 Issue 2 Pages 147-152
    Published: June 14, 1997
    Released on J-STAGE: August 11, 2009
    JOURNAL FREE ACCESS
    This report describes a rare case of the right-sided aortic arch with the right subclavian artery as the last branch, which was encountered in a Japanese male cadaver in the dissecting room at Kurume University School of Medicine in 1995. In this subject, the ascending aorta arose from the left ventricle and ascended obliquely, curving forward and to the right, and became the right-sided aortic arch. The aortic arch passed upwards, reaching a summit at the level of the third thoracic vertebral body, then curved dorsally. The left brachiocephalic, the right common carotid and the right subclavian arteries came off the aortic arch in that order. After the right subclavian artery, the aortic arch dilated and formed the aortic diverticulum behind the trachea and the esophagus. The right thoracic aorta, a continuation of the aortic arch, descended to the right of the vertebral column. This case is a type M anomaly according to the Adachi-WilliamsNakagawa's classification, and is the twelfth case with this type of vascular variation to be reported in Japan.
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  • KATSUMARO AIDA, TSUYOSHI SAGA, KOH-ICHI YAMAKI, YOSHIAKI DOI, TETSUSHI ...
    1997 Volume 44 Issue 2 Pages 153-156
    Published: June 14, 1997
    Released on J-STAGE: August 11, 2009
    JOURNAL FREE ACCESS
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