巻号一覧
48 巻 (2001)
- 4 号 p. 255-
- 3 号 p. 193-
- 2 号 p. 87-
- 1 号 p. 1-
48 巻, 2 号
選択された号の論文の16件中1～16を表示しています
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KOSUKE USHIJIMA, AKIHIKO KIMURA, TAKAHIRO INOKUCHI, YASUHIKO YAMATO, K ...2001 年 48 巻 2 号 p. 87-91
発行日: 2001/06/15
公開日: 2009/08/11
ジャーナル フリーTo investigate the role of placental transport of bile acids in fetal bile acid metabolism, such as with regard to synthesis of the unusual bile acids (1β-and 6α-hydroxylated and unsaturated bile acids), we measured the concentrations of bile acids in umbilical cord blood, amniotic fluid, maternal serum and maternal urine at delivery by means of gas chromatography-mass spectrometry. Serum and urine from healthy nonpregnant women were used as controls. We detected large amounts of unusual bile acids, especially hyocholic acid and 3β-hydroxy-Δ5 bile acids, in amniotic fluid and umbilical cord blood. The concentration of total bile acids in maternal serum was less than that of control serum and umbilical cord blood, and the concentration of total bile acids in maternal urine was higher than that of control urine and amniotic fluid. In conclusion, the fetussynthesized large amounts of unusual bile acids, and these compounds were transported from fetus to mother by placental transfer. We suggest that pregnant women may excrete large amounts of bile acids into the urine to control serum concentration of bile acids in fetus.抄録全体を表示PDF形式でダウンロード (687K) -
YASUMI ARAKI, HIROHARU ISOMOTO, KAZUO SHIROUZU2001 年 48 巻 2 号 p. 93-98
発行日: 2001/06/15
公開日: 2009/08/11
ジャーナル フリーIn the recent studies associated with the modulation of 5-fluorouracil (5-FU) and the development of new antifolates, attentions have been focused on the expression of the target enzymes, thymidylate synthase (TS) and dihydropyrimidine dehydrogenase (DPD), that affect tumor sensitivity and resistance to drugs. In order to evaluate predictability of therapeutic efficacy by intratumoral enzyme activity, we investigated the role of TS content and DPD activity in tumor sensitivity to 5-FU. Surgical specimens were obtained from 51 patients with colorectal cancer and used to measure TS content and DPD activity. TS content and DPD activity in tissues were measured by [3H]-FdUMP binding assay and radioenzymatic assay, respectively. The sensitivity to 5-FU in tumor specimens was determined by collagen-gel droplet embedded-drug sensitivity test (CDDST). The TS content and DPD activity did not correlate with Dukes' staging. There was no correlation between TS content and DPD activity in any tumors. Simple linear regression analysis showed that neither DPD activity (r=-O.267, p>0.05) nor TS content (r=-O.277, p> 0.05) in tumors had a significant correlation with 5-FU effectiveness independently. Four out of 24 patients, highly responsive to 5-FU, showed low levels in both DPD and TS. The patients with high value in either DPD activity or TS content proved not to respond to 5-FU. In conclusion, these results demonstrate that both tumor DPD activity and TS content are the factors predicting 5-FU responsiveness in colorectal cancer.抄録全体を表示PDF形式でダウンロード (2021K) -
YASUMI ARAKI, NOBUYA ISHIBASHI, YUTAKA OGATA, KAZUO SHIROUZU, HIROHARU ...2001 年 48 巻 2 号 p. 99-103
発行日: 2001/06/15
公開日: 2009/08/11
ジャーナル フリーThe aim of this study was to evaluate the effectiveness of laparoscopic-assisted total colectomy with ileal J-pouch anal anastomosis in comparison with that of conventional open total colectomy in patients with ulcerative colitis. From 1990 until 1999, 11 patients underwent open total colectomy, while 21 patients had laparoscopic-assisted total colectomy. Patients characteristics, perioperative course, and recovery were compared. Differences between the groups were tested using Student's t-test for independent groups and chi-square tests when appropriate. Nasogastric tube could be removed after POD 1.1 vs. 4.8 (p<0.05), the mean time to passage of stool was 1.7 (range, 1 to 3) vs. 5.4 (range, 3 to 7) days (p<0.05), and in the laparoscopic group watery stool was soon made solid after POD 24.3 vs. 87.3 (p<0.01). There were no significant differences in the mean operating time and mean estimated operating blood loss between two groups. Postoperative morbidity did not differ significantly between the patients treated conventionally (45.5%) and laparoscopically (33.3%). The findings of this study indicate that laparoscopic-assisted total colectomy improved cosmetic results and to be a safe and effective treatment for the elective surgery of ulcerative colitis.抄録全体を表示PDF形式でダウンロード (686K) -
The Standard Renal Volume of Japanese Boys and Girls Determined by Three-Dimensional UltrasonographyTAKAYO NOMASA2001 年 48 巻 2 号 p. 105-110
発行日: 2001/06/15
公開日: 2009/08/11
ジャーナル フリーWe calculated the volume of the kidney in 442 kidneys in 221 normal children (15neonates, 45 infants, 82 boys and 79 girls between 1 and 15 years of age) by using three-dimensional ultrasonography. The standard curves of the renal volume by height and age were determined. No differences in the renal volumes were observed between boys and girls from 1 to 14 years of age, whereas the difference between volume in boys and girls 15 years of age was significant; the boys had larger kidneys. A significant correlation between height and the volume of the right kidney (r=0.756, P<0.0001), and between height and the volume of the left kidney(r=0.791, P<0.0001) was observed. In addition, a significant correlation was found between the renal volume and the standard renal weight in male and female Japanese children. The results provide a new standard for renal volume in Japanese children. It is considered that this standard curve might be useful in a clinical setting, as a reference for evaluating patients with renal diseases that may affect the volume of the kidneys.抄録全体を表示PDF形式でダウンロード (2083K) -
WAKAKO HIMENO2001 年 48 巻 2 号 p. 111-116
発行日: 2001/06/15
公開日: 2010/02/08
ジャーナル フリーPrevious studies have demonstrated that the expression of angiogenic growth factors is induced in hypoxic models. However, little is known about these factors in patients with cyanotic heart disease. The purpose of this study was to examine the relationship between the plasma level of angiogenic growth factors and the severity of cyanosis. The study included 85 patients with cyanotic heart disease and age matched 81 controls. Median age was 4.2 years in the cyanotic group and 4.8 years in the control group. Mean systemic oxygen saturation was 80.6±7.3% in the cyanotic group and 98.1±0.5% in the control group. In the control group, vascular endothelial growth factor (VEGF) in the neonatal period was significantly elevated, then rapidly decreased within 3 months after birth. After 3 months of age, VEGF levels remained at a plateau. In contrast, this age dependency did not occur in hepatocyte growth factor (HGF) levels. Although VEGF and HGF levels were not different between the cyanotic and control groups within 3 months after birth, the VEGF level in the cyanotic group after 3 months of age was significantly elevated compared to the levels measured in the control group (149.2±105.6 vs. 66.3±22.5 pg/ml, p<0.0001). Moreover, the VEGF level was negatively correlated with oxygen saturation (y=440.6-3.53x, R=0.47, p<0.0001) in cases more than 3 months old. In contrast, no correlation was found between HGF level and oxygen saturation. Although physiologically increased VEGF in the neonatal period was rapidly decreased under normal oxygen saturation, a higher VEGF level persisted if systemic hypoxia was present. Persistently higher VEGF level may be related to the development of systemic to pulmonary collateral arteries in patients with cyanotic heart disease.抄録全体を表示PDF形式でダウンロード (3043K) -
HIROTO JOJIMA2001 年 48 巻 2 号 p. 117-127
発行日: 2001/06/15
公開日: 2009/08/11
ジャーナル フリーThe early diagnosis of 58 patients with hematological neoplasms accompanied by severe pulmonary infections of Pneumocystis carini (Pc), or Cytomegalovirus (CMV) pneumonia was made by polymerise chain reaction (PCR) using sputum samples, and of pulmonary mycosis by measuring blood β-glucan levels by a Limulus test. The effectiveness of early treatment for opportunistic infection based on these early stage diagnosis was evaluated and the results of pathological analyses of the lung at autopsy were analyzed. PCR identified Pc pneumonia in 7 of the total 58 patients (12.1%), and early treatment was effective in all 7 patients (100%). PCR identified CMV pneumonia in 5 patients (8.6%), and early treatment was effective in 2 of the 5 patients . The level of β-glucan confirmed mycotic pneumonia in 9 of these 58 patients (15 .5%), and early treatment was effective in 7 of these (66.7%). These findings indicate that PCR and the β-glucan method effectively enabled clinicians to diagnose pulmonary opportunistic infection in the early stage in 21 of the 58 patients (36.2%), and that early treatment was effective in 16 of the 21 patients (76.2%). The results of the pathological analyses of the lung at autopsy were: pulmonary tumor cell infiltration in a total of 5 patients (2 with ATL, 2 with NAE and 1 with AML); infection in a total of 6 patients (2 with ML and 4 with ATL); and diffused alveolar damage in a total of 4 patients(2 with ML, 1 with ATL and 1 with AML). CMV infection was confirmed in a total of 5 patients (2 with ML and 3 with ATL), and mucormycosis in a total of 2 patients (1 with ML and 1 with ATL) . Despite these findings, Pc and other fungi or bacteria were not detected. Early diagnosis and treatment by the present PCR and .β-glucan method were useful, but the underlying disease and its disease state influenced the clinical outcomes of patients with terminal pulmonary infection caused by CMV or mucor, suggesting that prevention and early diagnostic measures for these infections remain to be established.抄録全体を表示PDF形式でダウンロード (8020K) -
SHIGEKI FUKAHORI2001 年 48 巻 2 号 p. 129-134
発行日: 2001/06/15
公開日: 2010/02/08
ジャーナル フリーThe measurement of Wilms' tumor gene (WT1) mRNA levels by reverse transcriptase-polymerase chain reaction (RT-PCR) is useful in detecting minimal residual disease (MRD) in leukemia patients. In the present study, we quantified the level of WT1 mRNA in the peripheral blood and bone marrow of patients with acute myelocytic leukemia (AML) at initial onset, remission and recurrence by the use of nucleic acid sequence based amplification (NASBA), and then ascertained the clinical usefulness of this method. At initial onset, the level of WT1 mRNA in the peripheral blood was above 103 copies/μg and that in the bone marrow was above 104 copies/μg. The level of WT1 mRNA was decreased in cases where therapy resulted in complete remission, but it was abnormally high in recurring cases. In AML (M3) patients, the relationship between the level of WT1 mRNA and the expression of the PML-retinoic acid a receptor (RARα) gene, assessed by fluorescence in situ hybridization (FISH), was investigated. When leukemia was in remission hematologically, the PML-RARα gene was negative and the level of WT1 mRNA decreased. These findings suggest that the quantification of WT1 gene expression by competitive NASBA is useful in assessing therapeutic effects and detecting MRD.抄録全体を表示PDF形式でダウンロード (2046K) -
JUN FURUI2001 年 48 巻 2 号 p. 135-143
発行日: 2001/06/15
公開日: 2010/02/08
ジャーナル フリーKawasaki disease (KD) is an acute, self-limiting systemic vasculitis syndrome ofunknown origin, that mainly affects small and medium-sized arteries, particularly the coronary artery, which affects primarily infants and young children. Cell adhesion molecules play important roles in the inflammatory process. The aims of this study were to investigate the pathophysiological role of cell adhesion molecules in KD, and to look for the evidence of direct relationship between the plasma levels of soluble cell adhesion molecules and the incidence of coronary artery lesion (CAL). The 52 patients with KD, Group A patients who were clinical responders of initial intravenous immunoglobulin (IVIG) treatment (n=30), Group B patients who did not respond to the initial IVIG treatment (n=22), were studied. The circulating E-selectin (105.6±12.6 ng/ml) in the acute phase of KD, while the peak plasma P-selectin level (238.4±26.8 ng/ml) occurred in the subacute phase of illness (p<0.05, respectively). Plasma L-selectin levels (1557.3±44.3 ng/ml) during the convalescent phase tend to higher than in the acute and in the convalescent phases (p=NS). The analysis of paired samples in Group A patients before (E-selectin: 131.2±9.8 ng/ml, P-selectin: 216.6±13.4 ng/ml) and 48 hour after (E-selectin: 98.9±9.2 ng/ml, P-selectin: 153.9±34.1 ng/ml) IVIG administration revealed significantly lower values of E-and P-selectins, however, no significant differences in those in Group B patients. There were also no significant differences in the values of L-selectins between the 2 groups. Before IVIG treatment, the plasma levels of E-(225.1± 46.1 ng/ml) and P-selectin (259.4±76.2 ng/ml) of patients with CAL (n=11) were significantly higher than those of patients (n=41) without CAL (p<0.05, respectively). Plasma L-selectin levels (1596.9±385.0 ng/ml) in patients with CAL tended to be lower than those in patients without CAL (p=NS). E-and P-selectin may have potential as predictors of CAL in patients with KD.抄録全体を表示PDF形式でダウンロード (1276K) -
HIROKI MOTOYAMA2001 年 48 巻 2 号 p. 145-150
発行日: 2001/06/15
公開日: 2009/08/11
ジャーナル フリーIt has been demonstrated that intercalated cells (ICs) change their cellular composition in acid stimuli in adult rabbits but not fully explained in developing rabbits. To clarify the mechanisms of adaptation in acid-base imbalance in collecting ducts in developing rabbit kidneys, we examined the cellular composition of cortical collecting ducts (CCDs) in 4-week-old rabbits. In the control group, the ratio of ICs to total CCD cells and that of peanut agglutinin (PNA) non-binding ICs to total ICs were 37.2±7.2% and 40.3±3.1%, respectively. By contrast, inconsistent with adult reports, in the acidotic group, these ratios were 38.4±5.1 % and 41.9±1.7%, respectively, similar to the control group. The urinary pH in the control group was 8.20±0.14, while that in acidemia was 4.98±0.33 (p<0.01). These data indicated that cellular remodeling of ICs in the acidotic state is less important for adaptation in the 4-week-old rabbit. Another mechanism, employing an acid-base related protein, might be playing an important role during development for acid base imbalance.抄録全体を表示PDF形式でダウンロード (5415K) -
HIROKO INADA2001 年 48 巻 2 号 p. 151-157
発行日: 2001/06/15
公開日: 2010/02/08
ジャーナル フリーPeripheral blood stem cells (PBSC) harvests were performed from children with various types of cancer in our institution. The PBSC was cryopreserved using the simplified method at-80°C with 6% hydroxyethyl starch (HES) and 5% dimethyl sulfoxide (DMSO) without a programmed freezer. To determine the optimum cryo-storage time in this method, 33 samples harvested from 33 patients were investigated. While there was no correlation between the time of cryopreservation and the nucleated cell recovery rates, the CFU-GM recovery rate was negatively correlated with the cryopreservation times (p<0.01). The CFU-GM recovery rate after 2, 3, and 5years cryopreservation was 74%, 56%, and 21%, respectively. To date, 22 of the 33 children have subsequently received PBSCT using their cryopreserved cells. The number of infused CFU-GM of PBSCs was 6.9-114.6 (median 20.5)×104/kg. The cryopreservation time of infused PBSCs was 1-35 (median 4) months. After PBSCT, the absolute neutrophils count (ANC) achieved 500/mm3 between 8-16 (median 10.5) days, and the platelet count achieved 5.0×104/mm3 between 13-200 (median 29) days. We have experienced PBSCT using PBSCs after 35 months cryopreservation as the maximum and successful engraftment. It has been clarified in this study that PBSCs could be clinically used successfully after 3 years cryopreservation.抄録全体を表示PDF形式でダウンロード (5106K) -
KOICHIRO UEDA2001 年 48 巻 2 号 p. 159-164
発行日: 2001/06/15
公開日: 2009/08/11
ジャーナル フリーA microarray system is a powerful and very useful technology for analyzing the expression profile of thousands of genes. In this study, we made a cDNA microarray system carrying 2007 cDNAs obtained from primary neuroblastoma cDNA library and identified retinoic acid (RA)-regulated genes in a RTBM1 neuroblastoma cell line. We repeated independent hybridization experiment twice and found that 7 genes were up-regulated, and 5 genes were down-regulated on the cDNA microarray. The semi-quantitative reverse transcriptase (RT)-PCR analysis to confirm the results showed that 4 genes which included amyloid precursor-like protein 2 (APLP2), P311, dihydropyrimidinase related protein3 (DRP3) and RGP4 were up-regulated, while 2 genes, ld-2 and vimentin, were down-regulated. Thus, our neuroblastoma cDNA microarray system is useful to screen the neuronal differentiation- and growth-related genes regulated by RA with high efficiency.抄録全体を表示PDF形式でダウンロード (5823K) -
SHIGERU IKEZAWA2001 年 48 巻 2 号 p. 165-170
発行日: 2001/06/15
公開日: 2009/08/11
ジャーナル フリーWe examined the prevalence of Chlamydia pneumoniae in acute respiratory tract infection and association of C. pneumoniae infection and reactive airway disease in Japanese children. Four hundred eleven children with acute respiratory tract infection were enrolled in this study, and C. pneumoniae was isolated from 58 (14.1 %) patients by culture. Evidence of infection with C. pneumoniae was detected in 58 children with pneumonia (34.5%), bronchitis (41.4%) and upper respiratory tract infection (24.1%). Twenty-nine (50.0%) out of 58 patients were younger than 5 years old and 18 (31.0%) had wheezing at first visit. A logistic test for anti-C. pneumoniae-specific IgE showed the deference in the fluorescence unit between the patients with C. pneumoniae infection with and without wheezing was statistically significant (P0=0.02748, to=2.31 891). In conclusion, C. pneumoniae seems to be an important respiratory tract pathogen among young Japanese children, and our results support the association of C. pneumoniae infection and reactive airway disease.抄録全体を表示PDF形式でダウンロード (3029K) -
HIDEO TAKASU2001 年 48 巻 2 号 p. 171-174
発行日: 2001/06/15
公開日: 2010/02/08
ジャーナル フリーAn important issue for developing a vaccine therapy for human malignancy is identifying adjuvants that can elicit T-cell responses to peptides. The present study evaluates interferon-α(IFN-α) as a vaccine adjuvant. C57BL/6 mice were immunized subcutaneously with peptide derived from influenza virus (Flu) either with or without IFN-α using different vaccine formulations. IFN-α significantly enhanced cytotoxic T lymphocytes (CTL) induction in mice immunized with Flu peptide in incomplete Freund's adjuvant (IFA). Flu peptide administered continuously for 3 days by osmotic pump with IFN-α could elicit CTL induction, whereas either Flu peptide or IFN-α alone was non immunogenic. Furthermore, injection of the liquid formation of Flu peptide with IFN-α in phosphate-buffered saline (PBS) did not elicit CTL induction. These results suggest that the continuous administration of peptide and local delivery of IFN-α are important for efficient CTL induction, and that IFN-α is an effective adjuvant for peptide-based vaccines.抄録全体を表示PDF形式でダウンロード (601K) -
NOBUKUNI YOSHIDA2001 年 48 巻 2 号 p. 175-181
発行日: 2001/06/15
公開日: 2010/02/08
ジャーナル フリーAlthough γ/δ T-cells are known to contain the highest frequency of mycobacteria-reactive cells in humans, and recent studies have suggested that they play an important role in the initial immune response to Mycobacterium tuberculosis (Mtb), very few studies have attempted to analyze these cells in patients with active pulmonary tuberculosis (TB). The aim of the present study was therefore to evaluate the γ/δ T-cell populations present in the peripheral blood and the IFN-γ production of γ/δ T-cells stimulated by PMA before and after anti-TB chemotherapy in patients in the initial treatment stage for primary active pulmonary TB. Cell populations were measured by three-color flow cytometry of peripheral blood mononuclear cells. We compared the population of γ/δ T-cells and the production of IFN-γ between normal healthy controls and TB patients. Absolute numbers of γ/δ T-cells remained constant in the peripheral blood of TB patients. However, production of IFN-γ in γ/δ T-cells was dramatically suppressed prior to anti-TB chemotherapy in comparison with healthy control subjects, and further reduced following anti-TB chemotherapy. We also examined the influence of isoniazid (INH) in anti-TB chemotherapy. INH suppressed IFN-γ production of γ/δ and γ/δ T-cells. The findings demonstrated a strong correlation between the production of IFN-γ in γ/δ T-cells and manifestation of primary active pulmonary TB, which was consistent with the hypothesized role for γ/δ T-cells in the protective immune response to Mtb infection.抄録全体を表示PDF形式でダウンロード (1151K) -
HISAFUMI KINOSHITA, SHINJI SATO, MITSUO HASHIMOTO, KOTARO HASHINO, TSU ...2001 年 48 巻 2 号 p. 183-187
発行日: 2001/06/15
公開日: 2009/08/11
ジャーナル フリーWe encountered a patient with an ileus caused by implantation of cancer cells after surgical treatment of bile duct carcinoma. The patient was a 55-year-old male diagnosed as having cancer in the lower bile duct who underwent pancreatoduodenectomy in March 1996. The comprehensive curability was B. The patient experienced nausea and vomiting and was diagnosed as having ileus in October 1998. The symptoms were alleviated by medical treatment. Because ileus symptoms recurred in December 1998 but were not alleviated by medical treatment, the patient was hospitalized in Kurume University Hospital for surgery. A mirror reflection was observed by plain radiography of the abdomen. Ultrasonography (US) revealed dilation of the intestinal duct and hypertrophy of the intestinal wall. An ileus tube was inserted but the symptoms did not improve, and therefore, the patient underwent surgery in February 1999. Upon laparotomy, a node of milk white color about 2 cm in size was found in the region coinciding with the enhancement on CT images, and part of the intestinal duct was found to be folded and adhered to the lower surface of the liver. No recurrence was observed in the liver, peritoneum, and lymph nodes. The tumor was excised together with part of the intestinal duct and liver to remove the ileus. At present, the patient is alive without recurrence. Because recurrence of cancer by implantation of cancer cells is often localized in a limited region, early diagnosis and excision are important.抄録全体を表示PDF形式でダウンロード (5573K) -
TERUO SASATOMI, TETSUHARU ORIISHI, RYOICHI NAKANO, YASUHIRO NOZOE, TOS ...2001 年 48 巻 2 号 p. 189-192
発行日: 2001/06/15
公開日: 2009/08/11
ジャーナル フリーIntussusception accounts for almost all cases of intestinal obstruction in children. In contrast, intussusception in adults is relatively rare. An 86-year-old Japanese female with rectal bleeding came to our hospital via ambulance. At first, colonoscopy findings revealed the sigmoid colon cancer. Ultrasonography showed a hypoechoic mass with a multiple concentric ring sign. Computed tomography showed a round fluid-filled cystic structure. Colon contrast studies demonstrated stenosis in the rectosigmoid colon. A laparotomy was performed. The sigmoid colon was intussuscepted to the rectosigmoid colon. We employed both rectosigmoid and sigmoid colon resection. The resected specimen showed that the disease was advanced sigmoid colon cancer with ulcer formation due to an ischemic change. Tumor was 4.5 cm×2.0 cm in size. The disease was histopathologically diagnosed as advanced sigmoid colon cancer, well-differentiated adenocarcinoma. We report here a case of adult intussusception due to the sigmoid colon cancer.抄録全体を表示PDF形式でダウンロード (6027K)
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