巻号一覧
49 巻 (2002)
- 4 号 p. 167-
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49 巻, 4 号
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Laparoscopic Intraperitoneal Repair of Postoperative Ventral Incisional Hernia Using ComposixTM MeshYASUMI ARAKI, NOBUYA ISHIBASHI, MASAMITU KANAZAWA, YUKIYA KISHIMOTO, K ...2002 年 49 巻 4 号 p. 167-170
発行日: 2002/12/16
公開日: 2009/08/11
ジャーナル フリーThis report describes the technique and early results obtained with a simple laparoscopic intraperitoneal onlay ComposixTM mesh repair for postoperative ventral hernia. ComposixTM mesh is constructed from one layer of polypropylene mesh and another layer of expanded polytetrafluoroethylene (ePTFE). From March 2000 to October 2001, we performed laparoscopic repair of postoperative ventral hernia in 9 patients. Four (44%) of these patients had a history of at least one failed hernia repair. The size of the abdominal wall defect varied from 4×5 cm to 10×12 cm (median, 8×9 cm). In all cases, the ComposixTM mesh (Bard Inc. USA) was stapled to the peritoneal surface of the abdominal wall, leaving the sac in situ. No death occurred as a result of surgery. Intraoperative small bowel injury occurred in one patient (11.1 %) for whom surgery was converted to laparotomy and small bowel resection. No infection was observed. The length of hospital stay varied from 5 to 10 days (median, 5.6 days). During the follow-up period of 8 to 15 months (median, 2 months), there was no recurrence of hernia. Laparoscopic ComposixTM mesh onlay repair is a safe, easy, and effective procedure with minimal discomfort and a low early recurrence rate.抄録全体を表示PDF形式でダウンロード (2686K) -
TOSHIHIKO YAMAUCHI2002 年 49 巻 4 号 p. 171-176
発行日: 2002/12/16
公開日: 2009/08/11
ジャーナル フリーTransforming growth factor-beta (TGF-β) is known as an important cytokine for scar formation in wound healing. The purpose of the present study was to investigate the effects of TGF-β and its neutralizing antibody on normal skin fibroblasts and scar-derived fibroblasts in culture. Endogenous TGF-β levels were similar in all fibroblasts. Cell proliferation increased when TGF-β1 or β2 was added to the cultures, and the increase was higher and started at a lower level in the scar-derived fibroblasts (p<0.05). The increase of fibroblasts was suppressed by the addition of TGF-β-neutralizing antibody to the cultures, and the suppression rate was higher in the scar-derived cells (p<0.05). Percentages of the cells in the growth phases of cell cycle decreased in the normal skin fibroblasts (p<0.05) when TGF-β-neutralizing antibody was added. Our findings showed that scar-derived fibroblasts and normal skin fibroblasts have a different sensitivity to TGF-β. Further study is needed on the effect of the neutralizing antibody to the cell counts and cell cycle of scarderived fibroblasts.抄録全体を表示PDF形式でダウンロード (2527K) -
KEISUKE OHTA, TETSUO INOKUCHI, YUUHO HAYASHIDA, TETSUYA MIZUKAMI, TOMO ...2002 年 49 巻 4 号 p. 177-183
発行日: 2002/12/16
公開日: 2009/08/11
ジャーナル フリーArachnoid granulation is a protrusion of the arachnoid membrane into the cranial sinus, and is thought to play an essential role in the cerebrospinal fluid (CSF) absorption. Because the cells covering the apex region of the arachnoid granulation have different morphological features compared to the ordinary endothelial cells lining of the cranial sinus lumen, it has been expected these covering endothelial cells perform some specific function in the CSF absorption mechanism. However, little is known about functional differences between the covering endothelium of the arachnoid granulation and the ordinary sinus endothelium. In the present study, the characteristics of the covering cells located at the apex of arachnoid granulations of human, monkey and dog brain were examined by histochemical and immunohistochemical methods. The endothelial cells lining the cranial sinus lumen generally expressed such proteins as von Willebrand factor (vWF), CD31 and glycoproteins containing GS-1 or LE-1 lectin reacting sugar residue which are endothelial cell markers. However, the endothelial cells specifically located at the apex of arachnoid granulations failed to show vWF immunoreactivity, whereas the other endothelial markers were positive in each species we examined. Double staining of vWF antibody with other markers has clearly demonstrated that the endothelial cells on the apex region of arachnoid granulations exhibit no expression of vWF whereas cells lining the lateral region of arachnoid granulations and the luminal surface of ordinary cranial sinuses showed co-localization of these markers. The structural and histochemical differences between endothelial cells located at the apex region of arachnoid granulations and those of the sinus wall may reflect functional differences.抄録全体を表示PDF形式でダウンロード (7681K) -
TAKASHI KUWAHATA, MASARU ISHIMATSU, YURI KIDANI, TAKASHI AKASU2002 年 49 巻 4 号 p. 185-190
発行日: 2002/12/16
公開日: 2010/02/08
ジャーナル フリーEffects of methylphenidate (MPH), an agent used clinically for the treatment of children presenting the attention-deficit/hyperactivity disorder (AD/HD), on synaptic transmission in the rat locus coeruleus (LC) were examined by intracellular recording methods. Bath-application of MPH (30 nM-3 μM) increased the amplitude of the inhibitory postsynaptic potential (IPSP), while it did not change the amplitude of the excitatory postsynaptic potential (EPSP). MPH increased the timetopeak and the half-decay time of the IPSP in LC neurons. MPH increased the amplitude of spontaneous IPSP: individual spontaneous IPSPs merged one into the other so as to produce regular, long-lasting waves of hyperpolarization. Clonidine (10 nM), a selective agonist for α2-adrenoceptors, depressed the IPSP without affecting the EPSP in LC neurons. The results suggest that MPH enhances inhibitory synaptic transmission in the rat LC by depressing the norepinephrine (NE) reuptake system.抄録全体を表示PDF形式でダウンロード (782K) -
SHINGO CHIHARA2002 年 49 巻 4 号 p. 191-199
発行日: 2002/12/16
公開日: 2010/02/08
ジャーナル フリーEffects of supplemental Sialyl Lewisx analogue, a major ligand for all three selectin family members, during warm blood Cardioplegia were assessed in the blood perfused isolated rat heart. The isolated hearts were arrested for 60 min with warm blood cardioplegia given at 20-min intervals. This was followed by 60 min of reperfusion. The hearts were divided into the following two groups according to the supplemental drugs added to the cardioplegic solution. The control group (n=6) received standard warm blood cardioplegia. The Sialyl Lewisx analogue group (n=6) received warm blood cardioplegia supplemented with Sialyl Lewisx analogue (60 μg/ml). Cardiac function, endothelial function, myocardial metabolism and myocardial myeloperoxidase activity were assessed before and after cardioplegic arrest. Left ventricular developed pressure and dp/dt were significantly (p<0.05) greater and -dp/dt was significantly (p<0.05) lower in the Sialyl Lewisx analogue group than the control group during reperfusion. Coronary flow at 15 min of reperfusion and NO production, when acetylcholine chloride was added were significantly (p<0.05) greater in the Sialyl Lewisx analogue group than the control group. Myeloperoxidase activity was significantly (p<0.05) lower in the Sialyl Lewisx analogue group than the control group. The results suggest that selectin-mediated endothelial-leukocyte interactions may play an important role in myocardial ischemia and reperfusion injury. Supplementation of Sialyl Lewisx analogue during warm blood cardioplegia may provide superior myocardial protection by suppressing leukocyte-endothelial interaction during early reperfusion period.抄録全体を表示PDF形式でダウンロード (1561K) -
SHUMEI CHIKAMA, SHIZUKA IIDA, MICHIRO INOUE, NOBUTOSHI KAWAGOE, KATSUR ...2002 年 49 巻 4 号 p. 201-210
発行日: 2002/12/16
公開日: 2010/02/08
ジャーナル フリーWe have reported that heparan sulfate (HS)/heparan sulfate proteoglycan (HSPG, syndecan-1) expression significantly increased in the rat kidney during calcium oxalate (CaOx) nephrolithiasis. Although the exact role of syndecan still remains unclear, HS/syndecan-1 is thought to have some important role in the CaOx crystal formation. Mardin-Darby canine kidney (MDCK) cells are most commonly used in kidney stone research. It was reported that MDCK cells do not express syndecan-1. In the present study, we established a novel MDCK cell line (KIC-synd-1) that expressed the human syndecan-1 gene. In this cell line, we confirmed stable expression of both sndecan-1 gene and core protein. Immunohistochemical study revealed positive staining of syndecan-1 monoclonal antibody in the basolateral and cytosolic area of the KIC-synd-1 cells. We also investigated the composition of glycosaminoglycans (GAGs) side chains in MDCK cells and KICsynd-1 cells by using high-performance liquid chromatography (HPLC). Four types of HS chains were identified in both cells as follows; ΔdiHS-NS, ΔdiHS-6S, ΔdiHS-diS1, ΔdiHS-diS2. Increased production of ΔdiHS-NS and ΔdiHS-diS2 were shown in KIC-synd-1 cells compared with production in MDCK cells (p<0.05). In contrast, only a small amount of ΔdiHS-6S and ΔdiHS-diS1 was contained in both cell lines. Total amount of HS was significantly increased in the KIC-synd-1 cells compare with that in the wild type MDCK cells (p<0.05). Scanning electron microscopy revealed no significant difference between cell surface of wild type MDCK cells and that of KIC-synd-1 cells in normal conditions. However, calcium oxalate crystal attachment was apparently decreased in the KIC-synd-1 cells. These results suggested that cell surface HS/syndecan-1 has preventive role for calcium oxalate nephrolithiasis via creation of a charge barrier against COM crystal attachment.抄録全体を表示PDF形式でダウンロード (11721K) -
MASAHIDE KAJI, YOSHIHIRO SATO, HARUKO KUNOH, AYA WATANABE, HISAMICHI A ...2002 年 49 巻 4 号 p. 211-216
発行日: 2002/12/16
公開日: 2010/02/08
ジャーナル フリーLittle is known about ischemic stroke occurrence in patients with myasthenia gravis (MG), although antiphospholipid antibodies are detectable in many MG patients. A 47-year-old woman with a 20-year history of generalized MG had an acute onset of right hemiparesis. She had undergone thymectomy 10 years previously and was treated for phlebothrombosis of the lower extremity 3 years previously. Computed tomography (CT) demonstrated an old infarct in the left frontal lobe and a new lesion in the right parietal lobe. Multiple small cortical and subcortical infarcts were demonstrated on fluid attenuated inversion recovery (FLAIR) images. Thrombocytopenia (5.9×10 4/μL), a prolonged activated partial thromboplastin time (aPTT; 50.2 sec), and an elevation of β2-lgG-glycoprotein I anticardiolipin antibodies (β2-GPIaCL; 55.7 U/mL) were observed. Neurological defects improved significantly over 2 weeks. She then was treated with oral prednisolone (30 mg/day) for 18 months, with resolution of laboratory abnormalities and no new cerebrovascular events or findings on imaging. We believe that our patient's multiple infarcts are caused by antiphospholipid antibodies and recommend glucocorticoid therapy to prevent recurrent of ischemic stroke in similar case.抄録全体を表示PDF形式でダウンロード (6402K) -
NAOFUMI ERIGUCHI, AKIRA MATSUNAGA, YASUHIKO FUTAMATA, KOSUKE TAYAMA, H ...2002 年 49 巻 4 号 p. 217-219
発行日: 2002/12/16
公開日: 2009/08/11
ジャーナル フリーAppendicitis can rarely occur in association with carcinoma of the caecum, particularly in elderly patients. We report a case of acute appendicitis provoked by an adenocarcinoma of the caecum which obstructs the lumen of the appendix in a 58-year-old man. The patient underwent an ilbocaecal reseciton with lymph node dissection. The diffculties of identifying a small tumor at laparotomy and the implication for optimal treatment are emphasized.抄録全体を表示PDF形式でダウンロード (3956K)
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