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Article type: Cover
1995 Volume 26 Issue 1 Pages
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Published: March 31, 1995
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Article type: Cover
1995 Volume 26 Issue 1 Pages
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[in Japanese]
Article type: Article
1995 Volume 26 Issue 1 Pages
1-2
Published: March 31, 1995
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[in Japanese]
Article type: Article
1995 Volume 26 Issue 1 Pages
3-4
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Masaki SHIMODA
Article type: Article
1995 Volume 26 Issue 1 Pages
5-14
Published: March 31, 1995
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Development of implant, which not only fills a bone defect, but very slowly releases an antibiotic and also provides the base for osteogenesis, is a very important clinical task. The purpose of this study is to obtain fundamental data necessary to develop such implant. Namely, we prepared combination implants using gentamicin (GM), polylactic acid(PLA), and hydroxyapatite(HAP), and then examined slow release of GM in vitro and effects on such sustained release and osteogenesis in vivo. Five kinds of implants were prepared and used in experiments. Implants were statically placed in a water bath at 37℃ in vitro ; each implant was implanted in a tibia of white rabbit in vivo. As a result, the most satisfactory results were obtained from implants of group E ; namely, these implants, containing 20mg HAP, 10mg GM, and 20mg PLA, were prepared as follows ; they were compressed, and then melted at 120℃ and cooled in teflon tubes for curing. In group E, GM was very slowly released in vitro over 2 months; GM was also released very slowly in vivo at a level exceeding MIC for 5 weeks, and a bone cortex defect was covered with an osteogenetic layer 6 weeks after implantation.
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Yoshiko MIZUKAWA, Jun HAYAKAWA, Takehiko KOMATSU, Kazuhito HAYAKAWA, Y ...
Article type: Article
1995 Volume 26 Issue 1 Pages
15-19
Published: March 31, 1995
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A statistical study of malignant melanoma was carried out on the 29 cases seen from 1974 to 1993 at the Department of Dermatology, Kyorin University School of Medicine. Data were analized regarding the age, sex, primary location of the tumor, clinical type, period between the onset and medical consultation, Breslow's tumor thickness, Clark's level, tumor stage and treatment. The incidence of the tumor has increased recently ; seventeen out of the 29 patients were seen in the latest 5 years. The mean age of the patients at the time of presentation was 59.6 years. The male-female ratio was 11 : 18. Acral lentiginous and nodular melanoma in the clinical type were most commonly seen in this study. Most of the patients were treated with the radical operation ; the regional lymph node dissection was performed in approximately 40%. More than half of the patients have not been followed up for a minimum of 5 years yet. Therefore, they should be carefully observated continuously.
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Yoshio SHIINA, Junko IIJIMA, Shyuichi KOHRI, Michi NAGOYA, Hiroshi YAM ...
Article type: Article
1995 Volume 26 Issue 1 Pages
21-27
Published: March 31, 1995
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Using HeLa 229 cells stained by 1-step silver staining method, the distribution of nucleolar organizer regions (AgNORs) in the nuclei was studied lightmicroscopically. The distribution of intranucleolar AgNORs (In-AgNORs) was classified into four types; the type filled with small grains, the type mixed with small and large grains, the type which has uneven distribution of large grains, the type with the localization of large grains to the nucleolar margin. There was an interrelationship between the nucleolar shape and the distribution of In-AgNORs. The majority (87.4%) of irregular shaped nucleoli were filled with small grains, but these small grains tended to decrease in round nucleoli. These results, suggested that the increased replication of rRNA in irregular shaped nucleoli filled with small grains, the frequency of which in the adenocarcinoma might reflect the cell proliferation as mitosis does. The number of the outer nucleolar AgNORs (satellite-AgNORs: S-AgNORs) was also related to the nucleolar shape. The numbers tended to decrease in irregular shaped nucleoli and to increase in round ones. The pyroninophilic areas around S-AgNORs in that stage suggested the replication of rRNA there and therefore might indicate the cell cycle in which the RNA replication is under way. Hereafter, as hereby presented the diagnostic value of the finding of In-AgNORs and S-AgNORs to estimate the phases in cycle and activities of the cells should be evaluated.
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Akikuni YAGITA
Article type: Article
1995 Volume 26 Issue 1 Pages
29-37
Published: March 31, 1995
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HLA typing is now becoming a valuable means in investigating the etiology and pathophysiology of autoimmune diseases and irremediable diseases. In this study the HLA antigens were determined in 170 patients with ulcerative colitis (UC), 100 with Crohn's disease (CD) and 64 with Behcet's disease (BD) to be compared with those obtained in 310 healthy individuals. The results indicate that UC has a significant correlation with HLA-A24, -B52, -DR2 and -DQ1 (corrected P<0.05). An HLA family study performed on 4 families (8 patients) with a high prevalence of UC revealed all patients who had developed the disease to share HLA-A24, -B52, -DR2 and -DQ1 haplotypes. On the other hand, a significant correlation was also noted to exist between CD and HLA-B61, -DR4 and -DQ3 (CP<0.05). An HLA family study of 4 families (8 patients) with a high prevalence of CD demonstrated all patients with manifest disease have HLA-DQ3 and either-B61 or -DR4. BD was shown to be significantly correlated with HLA-A31, -B51, -DR4 and -DR8 (CP<0.05). The frequencies of occurrence of HLA antigens were studies inBD patients according to the presence or absence of enterocolitis. The results indicate that BD with enterocolitis (IB) is frequently associated with HLA-CW3, while BD without enterocolitis (NIB) correlated with HLA-A31, although it is not a significant difference.
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Akira MIYAKAWA
Article type: Article
1995 Volume 26 Issue 1 Pages
39-49
Published: March 31, 1995
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By establishing an experimental animal model: of intraarterial infusion chemotherapy for metastatic liver cancer, we evaluated the significance of hepatic arterial infusion of Adriamycin (ADR) and assessed the applicability of hepatic arterial infusion to Methotrexate (MTX)/5-Fluorouracil (5-FU) sequential therapy. After rabbits received intrahepatic inoculation of 10^7 VX_2 tumor cells or intraportal inoculation of 10^6 cells, a catheter 0.61mm in diameter was inserted into the left gastric artery, and the other end of the catheter was pulled out of the back. The animals were housed in an original fixation box, which permitted experimental chemotherapy for 2 weeks. The hepatic arterial infusion of ADR 0.6mg/kg/ day for 5 days showed similar antitumor effect to that by one shot i.v. injection, but it was superior in safety with little distribution into the heart, bone marrow and skin. Concerning MTX(2mg/kg/day×3)/5-FU (1.2mg/kg/day×3) sequential therapy by hepatic arterial infusion, however, it was only suggested that bone marrow toxicity was reduced compared with i.v. injection. From these results, we conclude that this new experinental model is useful for preclinical studies of intra-arterial chemotherapy for metastatic liver cancer.
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Hidemi KOIKE, Hiroshi IRIE
Article type: Article
1995 Volume 26 Issue 1 Pages
51-58
Published: March 31, 1995
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To investigate the somatotopy of fingers in area 3b, we examined topographic difference of somatosensory evoked potentials (SEPs) by stimulation of each finger in 5 normal adults. Fingers of the right hand were stimulated individually from thumb to little finger in order. SEPs were recorded from 19 electrodes placed on the scalp according to the international 10-20 system, referred to average potentials, and interpeak amplitude values between N 20 and P 25 were mapped. To detect the mapping difference among fingers clearly, we drew a line connecting maximal negative and positive point on the map (M-M line), and measured the angle between M-M line and the sagittal line. This angle means the dipole direction projected on the scalp. Another check point is the ditection of reversed phase point on the M-M line, that is supposed to be located just above the position of the holizontal dipole. The reversed phase point on each map of each finger stimulation was shifted from the point in the vicinity of C3 to more posteromedially in order. Because the somatotopy of each finger on area 3b is arranged from lateral to medial in order, our results are consistent with this somatotopy. The direction of M-M line evoked by each finger stimulation was clockwisely changed in sequence. This arrangement means that area 3b is curved with anterior convexity at the hand area. The method used in the present study may be applied to the non-invasive evaluation of the plasticity in the primary somatosensory cortex.
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Makiko MURATA
Article type: Article
1995 Volume 26 Issue 1 Pages
59-75
Published: March 31, 1995
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Just downstream of the divE gene for tRNA_1^<Ser> which locates at 22 min on Escherichia colt chromosome, the existence of open reading frame (ORF) of 657 bases was reported. By northern hybridization and maxicell method, it was shown that the cmp gene was transcribed and translated into a 23.5 kDa membrane protein. Disruption of the cmp gene by inserting the kanamycin resistant gene (Km^r) was attempted. Southern hybridization analysis of the Km^r derivatives showed that the cmp gene on the chromosome was not replaced by 2.6kb Km^r containing fragment but that the introduced fragment was inserted near the cmp gene. The results indicate although not conclusively, that the cmp gene may be indispensable for growth. Isolation of the homologous gene of the cmp from Bacillus subtilis by hybridization method was also tried. 3.3kb fragment cloned includes the 631 base stretch which shows 48% homology to the cmp cording region. An open reading frame was found including this region but there was no homology of amino acids sequences between two genes, indicating that it was not a homologue of the cmp gene.
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Yoshitaka NAKATA, Tomoyuki GOYA, Tateo HANAOKA, Satoru HAMAKUBO, Syoji ...
Article type: Article
1995 Volume 26 Issue 1 Pages
77-81
Published: March 31, 1995
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A 68-year old female who noticed a sense of obstraction on swallowing consulted a nearby hospital. She was diagnosed as having an esophageal submucosal tumor through esophageal fluoroscopic and endoscopic examinations. The patient was referred to our department because of aggravation of symptoms four years after the initial examination. Fluoroscopic and endoscopic examinations, together with endoscopic ultrasonography revealed a sessile smooth surface submucosal tumor with a longitudinal diameter of approximately 3 cm in the cervical esophagus. Through a left cervical incision, the tumor was enucleated by applying longitudinal excision without leaving any impairment to the esophageal mucosa. The tumor was a soft consistent mass enclosed with a thin capsule and measuring 2.5×1.0×0.8 cm. The pathological finding showed a benign esophageal lipoma consisting of mature adipose tissue. The necessity of early treatment must be stressed as, if left untreated, the tumor will grow to a pedunculated state and it may give rise to a serious fatal symptom such as suffocation caused by obstruction of the larynx in regurgitation of the pedunculated tumor.
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1995 Volume 26 Issue 1 Pages
83-84
Published: March 31, 1995
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1995 Volume 26 Issue 1 Pages
84-
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1995 Volume 26 Issue 1 Pages
84-
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1995 Volume 26 Issue 1 Pages
85-
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1995 Volume 26 Issue 1 Pages
85-
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1995 Volume 26 Issue 1 Pages
86-
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1995 Volume 26 Issue 1 Pages
86-
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1995 Volume 26 Issue 1 Pages
86-87
Published: March 31, 1995
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1995 Volume 26 Issue 1 Pages
87-
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1995 Volume 26 Issue 1 Pages
87-
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1995 Volume 26 Issue 1 Pages
87-88
Published: March 31, 1995
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1995 Volume 26 Issue 1 Pages
88-
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1995 Volume 26 Issue 1 Pages
88-89
Published: March 31, 1995
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1995 Volume 26 Issue 1 Pages
89-
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1995 Volume 26 Issue 1 Pages
89-
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1995 Volume 26 Issue 1 Pages
89-90
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1995 Volume 26 Issue 1 Pages
90-
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1995 Volume 26 Issue 1 Pages
90-91
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1995 Volume 26 Issue 1 Pages
91-
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1995 Volume 26 Issue 1 Pages
91-92
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1995 Volume 26 Issue 1 Pages
92-
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Article type: Article
1995 Volume 26 Issue 1 Pages
92-
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Article type: Article
1995 Volume 26 Issue 1 Pages
93-
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Article type: Article
1995 Volume 26 Issue 1 Pages
93-
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Article type: Article
1995 Volume 26 Issue 1 Pages
94-
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Article type: Article
1995 Volume 26 Issue 1 Pages
94-
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Article type: Article
1995 Volume 26 Issue 1 Pages
95-
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Article type: Article
1995 Volume 26 Issue 1 Pages
95-
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Article type: Article
1995 Volume 26 Issue 1 Pages
96-
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1995 Volume 26 Issue 1 Pages
96-
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Article type: Article
1995 Volume 26 Issue 1 Pages
96-97
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1995 Volume 26 Issue 1 Pages
97-
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Article type: Article
1995 Volume 26 Issue 1 Pages
97-98
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Article type: Article
1995 Volume 26 Issue 1 Pages
98-
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Article type: Article
1995 Volume 26 Issue 1 Pages
98-99
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Article type: Article
1995 Volume 26 Issue 1 Pages
99-
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Article type: Article
1995 Volume 26 Issue 1 Pages
99-
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Article type: Article
1995 Volume 26 Issue 1 Pages
99-100
Published: March 31, 1995
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