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Volume 38 (2007)
- Issue 4 Pages 75-
- Issue 2+3 Pages 29-
- Issue 1 Pages 2-
Volume 38, Issue 2+3
Displaying 1-5 of 5 articles from this issue
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Review Article
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Haruo HOMAREDA2007 Volume 38 Issue 2+3 Pages 29-36
Published: September 30, 2007
Released on J-STAGE: August 13, 2008
JOURNAL FREE ACCESSNa+/K+-ATPase, which is present in animal cell membranes, actively transports Na+ from the inside to the outside of cell, contrary to K+ in the reverse direction using the energy derived from the ATP hydrolysis. Oligomycin increases the affinity for Na+ of the ATPase but not the affinity for K+. This antibiotic inhibits the Na+ transport but little inhibits the K+ transport by Na+/K+-ATPase. In addition, this antibiotic inhibits Na+/K+-ATPase activity without clear effects on the partial reactions of the ATPase, which are the Na+-dependent phosphorylation and the K+-dependent dephosphorylation. The addition of Na+ and oligomycin stimulates the high-affinity K+-dependent dephosphorylation. The inhibitor of the type such as oligomycin is not known elsewhere. The study on the inhibition mechanism of oligomycin should contribute to elucidate the ion transport mechanism by Na+/K+-ATPase. In this paper, I review the interaction of oligomycin with Na+/K+-ATPase.View full abstractDownload PDF (496K)
Original Articles
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Hideki GONDO, Katsumi TSUCHIYA, Tetsuo SAKAI, Shinya NAGAMATSU, Akira ...2007 Volume 38 Issue 2+3 Pages 37-50
Published: September 30, 2007
Released on J-STAGE: August 13, 2008
JOURNAL FREE ACCESSTo clearify the mechanism of breast cancer proliferation under the influence of estrogen, we adopted a model system of MCF-7 cell line and phenol red in culture. MCF-7 cells were cultured with or without phenol red and harvested in the early logarithmic phase and in the late logarithmic phase. From these specific four groups of cells were extracted mRNA. The obtained mRNA specimens were transcripted to [33P]-labeled cDNA probes, which were hybridized to cDNA array arranged on a nylon membrane. In the 1176 genes on the array analyzed by ANOVA, thirty one genes were significantly different with each other between the two cell cultures with or without phenol red at the level of p < 0.03. In the signal transduction-related genes, the expressions of HTR1D, proenkephalin B, and ACHE gene were up-regulated, and COMT was down-regulated in the presence of phenol red. Concerning the transcription activators, the expressions of relB and TEF1 were reinforced by phenol red, while BARD1 and IRF2 gene were contrarily repressed. These changes in gene expression, especially in the cellular signaling and transcriptional regulator appear to be one of the cause of the malignant exacerbation of the cancer.View full abstractDownload PDF (802K) -
Hikari KAWAMATA, Shoichi AWA, Akira HOSAKI, Tadashi WARAGAI, Naoyuki W ...2007 Volume 38 Issue 2+3 Pages 51-60
Published: September 30, 2007
Released on J-STAGE: August 13, 2008
JOURNAL FREE ACCESSInhaled nitric oxide (NO) is the vasodilator to produce selective pulmonary vasodilation, Pulmonary hypertension (PH) is an important determinant of morbidity and mortality after surgery for congenital heart disease (CHD), CHD with left-to-right shunt may produce pulmonary artery smooth muscle hypertrophy and hyperplasia and pulmonary vasoconstriction, We compared pulmonary hemodynamicus of these CHDs under NO with the use of Windkessel model,
We categorized them into 3 groups, one with normal pulmonary artery pressure (PAP) and pulmonary flow (Qp)(Group A), one with normal PAP and increased Qp (Group B of ASD), and still another with PH and increased Qp (Group C),
Inhaled NO for high flow induced pulmonary hypertension can revert to normal the pathologic state of pulmonary circulation in terms of Windkessel parameters, Inhaled NO for pediatric cardiac patients before surgery can help estimate and predict the postoperative evolution of pulmonary vascular behavior,View full abstractDownload PDF (654K) -
Rafiqul ISLAM, Naohiko ANZAI, Sophapun EKARATANAWONG, Sunena KUNDU-SRI ...2007 Volume 38 Issue 2+3 Pages 61-68
Published: September 30, 2007
Released on J-STAGE: August 13, 2008
JOURNAL FREE ACCESSOrganic anion transporters play an essential role in the elimination of numerous endogenous and exogenous organic anions from the body. In the present study, we investigated the transport property of human organic anion transporter 4 (hOAT4) as a p-aminohippurate (PAH) transporter. For this purpose, we established and utilized cells derived from the second segment of mice proximal tubule stably expressing hOAT4 (S2 hOAT4). RT-PCR result shows the expression of hOAT4 in S2 hOAT4. [3H] estrone sulfate (ES) and [14C] PAH uptake via hOAT4 were increased with time and concentration dependent manner. Km of ES and PAH were 9.9μM and 2.2 mM, respectively. ES uptake via hOAT4 was inhibited by PAH with competetive manner (Ki = 4.3 mM). hOAT4-mediated PAH uptake was strongly inhibited by sulfate conjugates, non-steroidal anti-inflammatory drugs, bile acids, penicillin G, BSP, probenecid, ouabain and quinidine and moderately inhibited by cAMP, cGMP and cimetidine. No inhibitory effect by tetraethylammonium and β-estradiol was observed. The present study may provide the molecular basis of drug efflux pathway in human kidneys.View full abstractDownload PDF (367K) -
Ayako TONARI, Junko KUSUDA, Yasunobu NAKO, Yasushi MARUYAMA, Ikuo IKED ...2007 Volume 38 Issue 2+3 Pages 69-74
Published: September 30, 2007
Released on J-STAGE: August 13, 2008
JOURNAL FREE ACCESSPurpose: We reviewed the treatment outcome of 41 early-stage esophageal cancer (34 mucosa layer cases, 7 sub mucosa stage cases) for three treatment methods focusing on the effectiveness of brachytherapy for cases treated from January 1991 to December 2005.
Materials and Methods: The age of the patients ranged from 38 to 84 years old (median age 68 years) with 37 male patients and 4 female patients. The treatment methods reviewed were (1) External beam radiation therapy (EBRT) group: 5 cases, 60∼66 Gy (one case radiotherapy only, 3 cases with chemotherapy and 1 case with endoscopic therapy). (2) High dose rate remote after load system (RALS) endoesophageal brachyterapy group (BT) group: 9 cases (3 cases radiotherapy only, none case with chemotherapy, 6 cases with endoscopic therapy). Irradiation was 6∼20 Gy in 2 to 3 fractions. (3) Combined external beam radiation therapy and brachytherapy (EBRT + BT) group: 27 cases, after external beam radiation of 30∼66 Gy, patients were treated with 12∼18 Gy in 2 or 3 fractions by brachytherapy (17 cases radiotherapy only, 8 cases with chemotherapy, 2 cases with endoscopic therapy).
Results: There was not a significant difference in the cumulative survival rate between the BT and EBRT + BT groups. Sixteen cases of local recurrence or lymph node metastasis were observed for the 41 cases (39.0%) with a median time of 95 months (3∼186 months). Complications occurred for 12 of 41 cases (29.2%) with a median time of 20 months (3∼62 months) after the end of treatment. (Complication symptoms included mucosal ulceration, severe esophagitis, esophageal perforation, stenosis, fibrosis of mediastinal space and esophago-bronchial fistula.
Conclusions: For early stage esophageal cancer, external beam radiotherapy of about 60 Gy followed by brachytherapy appears to reduce both recurrence rate and complication rate.View full abstractDownload PDF (108K)
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