Both second stage larvae of M. incognita hatched in soil (SHL) and in water (WHL) were stored at 10°C and 25°C in a volcanic ash soil with a moisture content of 65%. After given periods, those larvae were recovered by applying the double-layer centrifu-gal-flotation method. The larval suspensions recovered were divided into two equal parts and half of each part was placed on filters made of a thin layer of absorbent cotton with a sheet of Japanese paper set in Baermann funnels for 72 hr at room temperature to count the numbers of living larvae. The other halves were observed under a compound micro-scope at magnifications of 60-150 and the amount of food reserves was estimated from the area of the dark parts in the intestines.
Under storage at 25°C, the number of living larvae in SHL gradually decreased and 44% of the initial number remained until 90 days. Food reserves were not consumed appreciably. On the other hand, both numbers of larvae and food reserves in WHL showed a rapid decrease during the first 10 day period, but a slow one thereafter. Numbers of larvae after 10 and 90 days were 40% and 10% of the initial values, respectively. Under storage at 10°C, numbers of larvae and food reserves decreased slightly regardless of the hatching conditions.
Based on the results obtained at 25°C, it is considered that some factors related to hatching may influence the survival of the nematode larvae, or that the existence of some microhabitats in soil allows for a longer survival.
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