We are unable to confirm the evidence of the cross-reaction between staphylo-cocci and M. tuberculosis reported by Maillard, Rothbard and others.

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Comparing 15 strains of Salmonella pullorum and 11 strains of Salmonella gallinarum in nutritional requirements the following results were obtained.
1. Three strains of S. pullorum and 6 strains of S. gallinarum did not require any growth factors, but of the other strains, a combination of 3 vitamins-niacin, thiamine and riboflavin-was necessary for growth.
2. By an elimination test a medium containing 7 amino acids-glycine, asparagine, glutamic acid, proline, cystine, arginine and tryptophan-was found to support a moderate growth of all tested strains.
3. S. pullorum is more exacting than S. gallinarum in both growth factor and amino acid requirement (proline, glutamic acid and cystine).

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1. H. pertussis Tohama variant as well as strain No. 29 demonstrate positive capsule staining similar to that of Phase I strain, while Phase III strain does nottake the stain.
2. Electronmicroscopic studies show no significant differences of structure among these strains of H pertussis.
3. All these variants and the Phase I strain produce sensitization to histamine in mice.

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A new method for counting cells by determining their DNA content is reported.
Because relatively small specimens are required, this method can be used for counting the cells contained in abdominal fluid or exudates of animals such as mice.

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The streptolysin forming system, reported by Bernheimer, containing resting streptococcal cells, RNA, maltose, KH₂PO₄ and MgSO₄ was reexamined. It was found that the optimum concentration of potassium ions for streptolysin formation was much higher than that of phosphate ion, which led to the improvement of the streptolysin-forming system by the use of a mixture of KCl and NaH₂PO₄ in substitution for KH₂PO₄.
The process of streptolysin formation occurring in the modified system with a higher concentration of KCl proved to consist of three distinguishable phase: initial inhibition phase, production phase, and reduction phase. The manifestation and duration of these phases were influenced by the concentration of potassium ion and the pH of the reaction medium, the influences exerted by these being dependent on each other.
The mechanism of potassium-and pH-effect was discussed.

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1) With the skin test antigen extracted from pertussis cells by heating in acid(pH 1.8) and through ammonium sulfate and picric acid precipitation, an absorption test was performed on pertussal capsular (K) and somatic (O) antigen to investigate the identity or differences between these antigens.
2) The skin test antigen completely absorbed K antibody in H. pertussis antiserum, but when heated at 100ª for 30 minutes, it lost the absorbing power.
3) The skin test antigen did not absorb O antibody in the above mentioned antiserum, proving non-relation between the two.

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Cultures of B. polymyxa containing sucrose as the substrate for growth produce increased quantities of 2, 3-butanediol if 0.01gm. of ZnSO₄ is present in the culture medium. This increase of 2, 3-butanediol formation has been observed up to 54 days. Cultures containing high concentrations of zinc ions show greater formation of 2, 3-butanediol up to 36 days of fermentation but afterwards the yield of the glycol decreases with the increasing period of fermentation.

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