Antigenic analyses of 5 species of the genus Saccharomyces were carried out by the slide agglutination method using monospecific or various absorbed antisera. Saccharomyces pastori possesses thermostable antigens 1, 3, 4, 5, 17, 24 and 25, but no thermolabile antigen. It should be transferred to the genus Pichia. The antigenic structure of S. elegans or S. veronae is thermostable antigens 1, 4, 5, 8, 10, 28, 32, and 1, 2, 8, 10, 14, 28, 32, respectively. The antigenic structures of S. fructuum and S. microellipsodes are identical, thermostable antigens 1, 2, 8, 10, 14, 28 and 31. All of the species do not have thermolabile antigen.
Comparative studies showed that the antigenic structures of several strains of various Saccharomyces species corresponded with the antigenic structures of each species used for antigenic analyses, except for mislabelled strains. Therefore, the antigenic structures of the species in the genus Saccharomyces can serve in the classi-fication of the genus. It is the hope of the authors that serological characteristics of yeasts will be given more prominence in yeast classification than at present.
Pichia krusei was found to be a new species, and is considered the perfect form of C. krusei.

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Growth of Leptospira icterohaemorrhagiae after replacing rabbit serum with dead cells of Reiter treponeme was nearly equivalent to that obtained by the medium containing 10 per cent rabbit serum. With disintegrated cells of Reiter treponeme, proliferation of L. icterohaemorrhagiae was also excellent, and the growth was not inhibited even when it was used in high concentration, contrary to the growth observed when using disrupted cells of mycobacteria. The growth factors within cells of Reiter treponeme were thermostable on heating at 121 C.

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