The chloramphenicol resistance gene (pp-
cat) was cloned from a transferable R plasmid of
Pasteurella piscicida, pSP9351, and the sequence of the gene was determined. Subcloning and deletion analysis localized the resistance gene, pp-
cat, to within a 2.3kb
HincII-
BamHI fragment. The fragment as a probe hybridized with the type I chloramphenicol acetyltransferase (CAT) gene and did not hybridize to CAT types II, III, and CAT-VA. The fragment hybridized to transferable R plasmids encoded with resistance to chloramphenicol, which were detected from
P. piscicida isolated in different years. Nucleotide sequences of the coding and flanking regions of pp-
cat (2031 bp) identified an open reading frame coding type I CAT of a molecular mass of about 25, 000Da. Comparison analysis of the sequences outside the
cat open reading frame showed also that pp-
cat has homology, in part, with the gene that coding for the endonuclease
EcoRII and those that flank the
cat gene derived from the
Acinetobacter baumannii chromosome.
抄録全体を表示