Two cholera cases were diagnosed using an enzyme-labeled oligonucleotide probe (ELONP) hybridization test for detection of cholera toxin gene (
ctx) in a clinical laboratory at Osaka Airport Quarantine Station. The ELONP test with suspicious colonies of
Vibrio cholerae O1 grown on TCBS or Vibrio agar plates gave positive result for
ctx within 3hr. We also tried to apply the ELONP test for direct detection of
ctx in their stool and their non-selective culture. Specimens from Case #1, which contained 5.9×10
5CFU/g of
V. cholerae O1 in the stool, cultured for 7-8hr or longer in alkaline peptone water or Marine broth at 37C, became positive for
ctx. On the other hand, specimens from Case #2, which contained 8.7×10
8CFU/ml (of
V. cholerae O1 in the stool), gave positive result in this stool itself without any further culture. These data suggest that the ELONP test provides successfully a more rapid and accurate means of identifying “toxigenic”
V.
cholerae O1 in a clinical laboratory.
抄録全体を表示