Serological variations were examined among 12 type or reference strains and 91 oral isolates of vitamin B6-dependent
Abiotrophia and Granulicatella spp. Rabbits were immunized with whole cells of 12 selected strains and 10 typing antisera were obtained, which were unreactive with the Lancefield group A to G antigen preparations. The reactivity of the antisera and autoclaved cell surface antigen extracts was tested by double diffusion in agar gel and a capillary precipitin test. These typing antisera categorized all
Abiotrophia defectiva strains, all except one
Granulicatella elegans strain, three-quarters of the
Granulicatella adiacens, and half of the
Granulicatella paraadiacens into 8 serotypes and 2 subserotypes. The
Granulicatella balaenopterae type strain was unserotypable. All
A. defectiva strains were serotype I, some of which were divided into subserotype I-1 and/or I-5. The
G. adiacens strains generally belonged to serotype II or III, and the
G. paraadiacens strains to serotype IV, V or VI. All
G. adiacens or
G. paraadiacens serotype II strains were also subserotype I-5. The
G. elegans strains were serotype VII or VIII. These
Abiotrophia and
Granulicatella serotypes were undetectable among 33 strains of the other 11 species including the bacteriolytic enzyme-producing but vitamin B6-independent strains of
Streptococcus,
Enterococcus,
Dolosigranulum and
Aerococcus. The proposed serotyping system for
Abiotrophia and
Granulicatella spp. would be helpful in the identification and classification of these unique coccal isolates in ecological and epidemiological studies.
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