Urinary leukotriene E
4 (LTE
4) levels in healthy volunteers were determined using liquid chromatography/tandem mass spectrometry (LC/MS-MS). LTE
4 and its internal standard were extracted by simple solid-phase extraction and analyzed using LC/MS-MS in the selected reaction monitoring (SRM) mode by monitoring the transitions between
m/z 438 and
m/z 333 for LTE
4 and between
m/z 441 and
m/z 336 for LTE
4-
d3. We detected LTE
4 (64.7±8.3 pg/mg creatinine,
n=13) in healthy human urine. Then, we determined the intra- (within one day) and inter-day (for two weeks) variation of the excretion rates of LTE
4 in healthy volunteers (
n=5). No significant variations in urinary LTE
4 excretion rates were observed in both examinations. In conclusion, LC/MS-MS is a rapid, accurate and sensitive method for determination of urinary LTE
4. The present results about urinary LTE
4 levels and variation of LTE
4 excretion in healthy volunteers can be helpful in evaluating the role of cysteinyl LTs in pathophysiological conditions.
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