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Volume 54 (2006)
- Issue 6 Pages 227-
- Issue 5 Pages 183-
- Issue 4 Pages 127-
- Issue 3 Pages 75-
- Issue 2 Pages 39-
- Issue 1 Pages 1-
Volume 54, Issue 4
Displaying 1-6 of 6 articles from this issue
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REGULAR PAPERS
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Tadashi ARII, Yasuko OISHI, Shuichi HASHIMOTO2006 Volume 54 Issue 4 Pages 127-132
Published: 2006
Released on J-STAGE: August 01, 2006
JOURNAL FREE ACCESSThermal desorption for an aromatic guest molecule of phenanthrene adsorbed in Y and X zeolites exchanged with cations such as Na+, Tl+ and K+ has been studied successfully by means of TG-DTA and temperature programmed desorption (TPD)-MS. The thermal desorption of phenanthrene from the zeolites took place at temperatures between 300°C and 800°C. The temperature of thermal desorption depended critically on the cations in the order of the temperature of Na+<Tl+<K+. On the contrary, the activation energy for the desorption of phenanthrene estimated in terms of the Ozawa method, was almost the same for the three cation-exchanged Y zeolites with the approximate values of 120 kJ mol-1. On the other hand, the activation energy for the desorption of phenanthrene from the cation-exchanged X zeolites was the larger than that obtained from the Y zeolites, and was estimated as approximately 140 kJ mol-1. It is concluded that the activation energy for the desorption of phenanthrene from the zeolites does not directly correlate with the charge-compensating cation in the zeolites and depends strongly on the framework structures of the zeolites.View full abstractDownload PDF (411K) -
Shuichi SHIMMA, Masaru FURUTA, Katsuhiko ICHIMURA, Yoshikazu YOSHIDA, ...2006 Volume 54 Issue 4 Pages 133-140
Published: 2006
Released on J-STAGE: August 01, 2006
JOURNAL FREE ACCESSProteomics data obtained by mass spectrometry are now being combined with spatial information. This report outlines a digestion procedure for tissue sections on polyvinylidenfluoride membrane and results of a direct tandem mass spectrometry of mouse brain sections in situ. We succeeded in sequencing the digested peptides such as myelin basic protein, histon H2A, and tubulin β4 directly from tissue sections and transferred membrane. Protein blotting method can provide protein denaturation during the transfer process with electrode heat and with detergent contained in a transfer buffer. In addition to denaturing proteins, chemical inkjet printing technology and a highly sensitive matrix-assisted laser desorption/ionization quadrupole-ion-trap time-of-flight tandem mass spectrometer enabled us to print solutions of enzyme in micro-scale areas on the membranes and obtain high quality data in mass spectrometry/mass spectrometry. This analytical methodology will provide an innovative approach to in situ proteome analysis.View full abstractDownload PDF (1261K)
INTEGRATED PAPERS
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Tohru YAMAGAKI, Hiroaki SUZUKI, Kazuo TACHIBANA2006 Volume 54 Issue 4 Pages 141-149
Published: 2006
Released on J-STAGE: August 01, 2006
JOURNAL FREE ACCESSNegative ions from neutral oligosaccharides were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The deprotonated molecule [M−H]- were decomposed in negative-ion MALDI using nor-harmane as a matrix, indicated that the in-source decay took place at the laser irradiation. The chloride ion adducts [M+Cl]- were stable enough to be detected by reflectron-mode, and postsource decay (PSD) spectra were measured from those ions. The PSD ions were detected as the deprotonated ion form rather than the chlorinated one, indicated that the deprotonated molecules were very labile and a reaction intermediate in the PSD. The in-source decay ions depended on the linkage types of oligosaccharides and their spectra could be useful to identify the linkage types. The PSD spectra were also useful.View full abstractDownload PDF (1070K) -
Motohiro SHIZUMA2006 Volume 54 Issue 4 Pages 151-165
Published: 2006
Released on J-STAGE: August 01, 2006
JOURNAL FREE ACCESSThe chiral discrimination abilities of various permethylated oligosaccharides were evaluated toward ammonium ions of esters of amino acids using the fast atom bombardment (FAB) mass spectrometry (MS)/enantiomer labeled (EL) guest method. The complexation properties of the selected permethylated oligosaccharides were examined by other spectroscopic methods such as 1H-nuclear magnetic resonance, and the structures of their complexes were discussed. The artificial chiral hosts were synthesized on the basis of the obtained information, and then one of them was applied to evaluation of optical purity of chiral ammonium salts using FAB mass spectrometry.View full abstractDownload PDF (2453K)
COMMENTARY
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Hiroshi MIYANO2006 Volume 54 Issue 4 Pages 167-173
Published: 2006
Released on J-STAGE: August 01, 2006
JOURNAL FREE ACCESSHigh-performance liquid chromatography (HPLC) is one of the most useful methods for both qualitative and quantitative analysis of individual compounds that may be present in a sample. Researchers can select an appropriate method in various combinations of stationary phases with mobile phases depending on an objective compound and other matrix in a sample. This review aims to provide the outline of separation analysis and HPLC, including the history of chromatography, HPLC device configuration, typical separation modes, i.e., reversed-phase, normal-phase, ion-exchanged, ion-pair reverse-phase, and size exclusion HPLC, typical methods for amino acid analysis, key parameters of chromatography performance, typical procedure for optimum separation condition, usefulness of column downsizing, and method robustness, to the beginners of HPLC operations.View full abstractDownload PDF (1002K)
Letter from the Editor-in-Chief
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Ken-ichi YOSHINO2006 Volume 54 Issue 4 Pages 175-182
Published: 2006
Released on J-STAGE: August 01, 2006
JOURNAL FREE ACCESSDownload PDF (736K)
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