Journal of the Mass Spectrometry Society of Japan
Online ISSN : 1880-4225
Print ISSN : 1340-8097
ISSN-L : 1340-8097
58 巻 , 1 号
  • Minoru UCHIYAMA, Minoru OGUCHI, Naotoshi YAMAMURA, Hiroko KODA, Thomas ...
    2010 年 58 巻 1 号 p. 1-11
    発行日: 2010/02/01
    公開日: 2010/02/15
    ジャーナル フリー
    The in vitro metabolism of rosiglitazone (RGZ) was studied in freshly isolated human, rat, and monkey hepatocytes. The metabolites of [14C]RGZ produced by incubation with hepatocytes were detected by radioactivity detection high-performance liquid chromatography. Seven metabolites (M1-M7) of RGZ were detected in human hepatocytes. The structures of the metabolites were elucidated by liquid chromatography/tandem mass spectrometry using electrospray ionization. The structural analysis demonstrated that M1, M2, and M4 were novel metabolites of RGZ. M1 was identified as a 2,4-thiazolidinedione (TZD) ring-opened N-glucuronide. M2 was identified as a TZD ring N-glucuronide. M4 was proposed to be a TZD ring-opened methylmercapto amide. Similarly, these metabolites were also detected in rat and monkey hepatocytes. To our knowledge, this is the first report on N-glucuronidation of TZD rings. Based on the structures of the metabolites, we propose the following novel in vitro metabolic pathways for RGZ: 1) N-glucuronidation of the TZD ring of RGZ to form M2 followed by hydrolysis to the TZD ring-opened N-glucuronide M1; and 2) methylation of the mercapto group of the TZD ring-opened mercapto amide to form M4.
  • 第5回 内殻励起反応
    和田 真一, 田中 健一郎
    2010 年 58 巻 1 号 p. 17-27
    発行日: 2010/02/01
    公開日: 2010/02/15
    ジャーナル フリー
    Core electron excitation possesses an attractive potential of inducing selective chemical bond breaking. Compared to valence excitation, core excitation takes place within a small area because of its spatial localization and atomic selectivity. Therefore, a specific atom in a molecule can be excited selectively. The core-excited state is quite unstable with an extremely high energy, so consecutive decay processes—Auger decay and ionic dissociation—can occur in the vicinity of the excited atom. From this concept, site-selective ionic fragmentation has been widely and successfully investigated in various core-excited molecular systems. This paper briefly describes the characteristics of core excitation and experimental methods, and then reviews representative recent studies about site-selective chemical bond breaking induced by core excitation.
Letter from the Editor