A combination of a direct liquid extraction using a small volume solvent and the electrospray ionization, is distinguished by its ability to rapid measurement of complex chemical components in biological samples and to visualize their distribution in a tissue section. In this review, the development of techniques and their application to biological research, which has been emerged since the early 2000s, is presented. In addition, an overview of ESI, the ion suppression of the mixture, and the acceleration of the specific chemical reaction in the charged droplet are presented. The future expectation for visualizing the multimolecular environment in biological systems is discussed.
Compounds included in the inner coating of canned coffee were analyzed with liquid chromatography/mass spectrometry. The mass spectrometer was a hybrid quadrupole and time-of-flight type and run data-dependent acquisition mode that acquired fragment ions for features with sufficient abundance. Interpretation of the data revealed the adsorption of food-derived compounds in the inner coating, as well as byproducts and additives of polymer. In addition to the presence of ethylene terephthalate cyclic oligomers, the presence of antioxidants such as 2,2′-methylenebis[6-(1-methylcyclohexyl)-p-cresol] was confirmed. The finding can be valuable for feature health risk assessment of food contact materials.