Cell membrane was isolated from porcine ventricles and its Ca
2+ binding and ATPase activities were investigated. The cell membrane (heart sarcolemma) exhibits Na
+-K
+activated ATPase and Ca
2+ stimulated. Mg-ATPase activities which suggest the presence of “Ca-pump”system.
Ca
2+ binding to the cell membrane was dependent on Ca
2+ concentration and analysis of binding curve revealed that the existence of two kinds of binding sites (high and low affinity sites). Mg
2+ increased Ca
2+ binding at the high affinity site whereas Na
+ decreased it. These actions of Mg
2+ and Na
+ may be physiologically important for the maintenance and transient change of intracellular Ca
2+ concentration at rest and excitation of the membrane
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