巻号一覧
29 巻 (2004)
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29 巻, 2 号
選択された号の論文の7件中1～7を表示しています
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中嶋 光敏2004 年 29 巻 2 号 p. 80-89
発行日: 2004/03/01
公開日: 2011/03/04
ジャーナル フリーMicrochannel (MC) arrays with various shapes and sizes were micro-fabricated using a single-crystal silicon substrate. An emulsion is prepared by simply forcing dispersed phase fluid into continuous phase fluid through the MC. Real-time optical observations of the emulsification process can be realized with a microscope video system. The grooved type MC and module were used to characterize MC emulsification, and straight-through MC and module were developed for higher productivity. O/W-emulsions can be obtained due to its hydrophilic surface property after MC oxidation. Hydrophobic MC modified by silanization can be also applicable to W/O-emulsions. Both monodisperse O/W-and W/O-emulsions with average droplets sizes ranging 3-90 p m and coefficients of variations lower than 10% have been successfully obtained. Emulsification was significantly affected by MC sizes and structures. Analysis by high-speed camera revealed that the structure of terrace is so important for instability at the outlet, and spontaneous droplet formation caused by interfacial tension. An oblong straight-through MC was highly applicable to emulsification. MC emulsification was also applied to productions of solid-lipid and polymeric microsheres, and microcapsules, showing high availability. The MC emulsification technology has great potential for food, pharmaceutical and chemical industries.抄録全体を表示PDF形式でダウンロード (3332K) -
中島 忠夫2004 年 29 巻 2 号 p. 90-97
発行日: 2004/03/01
公開日: 2011/03/04
ジャーナル フリーW/O/W emulsion is one of the most promising candidates as a drug carrier in drug delivery system. Strict size control of emulsion is very important technology for preparing a stable and safe emulsion drug as well as delivering drug or emulsion particle to target organ. Membrane emulsification was found very suitable technique for these purposes through many clinical applications to the hepatocellular carcinorma. However there are some technical issues yet such as guarantee of long-term stability and quality of hydrophobic surfactants. W/O/W emulsion will be able to contribute to drug delivery system and industries in near future when such technical issues are truly solved out.抄録全体を表示PDF形式でダウンロード (2096K) -
通阪 栄一, 神谷 典穂, 後藤 雅宏2004 年 29 巻 2 号 p. 98-104
発行日: 2004/03/01
公開日: 2011/03/04
ジャーナル フリーA new solid-in-oil-in-water (S/O/W) emulsion consisting of edible surfactants, an edible oil, and a water-soluble drug has been developed for oral administration of peptide/protein drugs. Insulin, which is a hydrophilic macromoleclule, was converted into a lipophilic complex using edible surfactants. As the resultant complex can be directly solubilized in the oil phase, surfactant-insulin complexes can be incorporated into the oil phase of oil-in-water emulsions, allowing the formulation of stable S/O/W emulsions. The capability of S/O/W emulsions in the control of blood glucose was investigated with diabetic model rats. The glucose level in the serum slowly decreased over several hours when the S/O/W emulsions were orally administered, while the aqueous solution of insulin (as the control) showed no significant effect on the blood glucose level. The hypoglycemic effect of surfactant-insulin complex incorporated into the S/O/W emulsions and the stable encapsulation of insulin into dry emulsion formulations will offer one way to oral delivery of biopharmaceuticals.抄録全体を表示PDF形式でダウンロード (1522K) -
油滴はいかなる時に活性充填物質として働くか合谷 祥一2004 年 29 巻 2 号 p. 105-113
発行日: 2004/03/01
公開日: 2011/03/04
ジャーナル フリーThe dispersed oil droplets in emulsion gel contribute to the rheological properties of emulsion gels either as active or inactive filler particles. For gels based on proteins, the oil droplets act as an active filler. For the case of polysaccharide gels, the oil droplets act as an inactive filler. The storage modulus G' of an acid-induced sodium caseinate emulsion gel dramatically decreases in the presence of the Tween 20, with the character of the surface of the emulsion droplets changing from active filler to inactive filler on addition of the surfactant. The effect of Span 20 is different from that of Tween 20, remarkably.抄録全体を表示PDF形式でダウンロード (1535K) -
Naftal Kilenga, Masahiko Kato, Sumie Manno, Akira Kaneko, Yuichi Takak ...2004 年 29 巻 2 号 p. 114-122
発行日: 2004/03/01
公開日: 2011/03/04
ジャーナル フリーRed blood cell (RBC) membranes are stable and deformable due to its mechanical properties maintained by the spectrin-based submembranous skeletal network. Therefore, RBC appears not to accept Plasmodium falciparum (Pf) invasion easily. However, Pf does invade RBC probably due to change in membrane mechanical properties at the area of Pf contact.
In the present study we examined whether increase in membrane rigidity may prevent Pf invasion into RBC. Wheat germ agglutinin (WGA) was utilized to increase membrane rigidity and double fluorescence assay with a flow cytometer was established; the biotin-WGA-treated RBC (B-WGA-treated RBC) was mixed with Pf-infected RBC followed by staining with streptavidin-FITC and propidium iodide (PI) to detect B-WGA-treated RBC and Pf-infected RBC, respectively.
Having confirmed that membrane rigidity of B-WGA-treated RBC increased significantly using an ektacytometer, we could show that these cells were not infected by Pf using double fluorescence assay. The target molecule of B-WGA was also identified to be band 3 by pull-down assay. These results enabled us to suggest that B-WGA binding to band 3 molecule may induce conformational change in band 3-ankyrin-spectrin, sequentially, resulting in increase in membrane rigidity and thus prevention of Pf invasion into RBC.抄録全体を表示PDF形式でダウンロード (1275K) -
松宮 紀文, 松藤 茂雄, 岡部 和弘, 真野 弘, 寺本 正明2004 年 29 巻 2 号 p. 123-130
発行日: 2004/03/01
公開日: 2011/03/04
ジャーナル フリーA novel carrier, 2, 3-diaminopropionic acid (DAPA), was proposed for the facilitated transport of CO2, and experiments on the separation of CO2 from a mixture of 10% CO2 and 90% N2 were performed using supported liquid membranes to evaluate the performance of this carrier. DAPA exists in four kinds of ionic forms in the aqueous solution depending on its pH. It was found that the membranes containing DAPA (C), which has one free amino group and the membrane containing DAPA (D), which has two free amino groups, showed much higher CO2 permeance Qco2 and selectivity of CO2 from N2 than the membranes containing monoethanolamine or Cs2CO3 as CO2 carriers. It was deduced from the observed solubility of CO2 in aqueous DAPA solutions that one mole of CO2 reacts with two moles of DAPA (C) and also that CO2 reacts with one mole of DAPA (D). Both Qco2 and selectivity increased with increasing temperature. The values of Qco2 and selectivity observed with the DAPA (C) membrane at 25°C were 1.00×10-4cm3 (STP) / (cm2s cmHg) and 650, respectively, and the corresponding values at 70°C were 1.79×10-4cm3 (STP) / (cm2s cmHg) and 1240, respectively. The membrane was table at high temperatures probably because the loss of DAPA from the membrane by its evaporation was very small due to its very low vapor pressure. These results suggest that DAPA is promising as a carrier for CO2.抄録全体を表示PDF形式でダウンロード (891K) -
-PMMA膜, ポリスルホン膜-和田 茂久, 菅谷 博之2004 年 29 巻 2 号 p. 131-136
発行日: 2004/03/01
公開日: 2011/03/04
ジャーナル フリーToray Industries have manufactured two types of high performance hollow fiber type membranes for artificial kidney. One is PMMA membrane (BK, BG series) and another is polysulfone membrane (BS series). In recent years, many dialysis patients' life came to be saved by progress of dialysis technologies. The number of long-term dialysis patients has increased and the frequent chronic complications of long-term dialysis patients have increased. β2-microglobulin was identified as a major constituent protein of amyloidosis which is one of the complications. PMMA membrane (BK, BG series) and polysulfone membrane (BS series) are designed to remove β2-microglobulin. PMMA membrane (BK, BG series) can remove β2- microglobulin by a high β2-microglobulin adsorption property. Polysulfone membrane (BS series) can remove β2-microglobulin by a high /β2-microglobulin penetration property. We design the form of artificial kidney suitable to the characteristics of PMMA membrane and polysulfone membrane.抄録全体を表示PDF形式でダウンロード (815K)
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