膜
Online ISSN : 1884-6440
Print ISSN : 0385-1036
ISSN-L : 0385-1036
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35 巻, 2 号
選択された号の論文の8件中1～8を表示しています
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特集：膜と膜機能の可視化
総説
原著
  • Homology Modeling of an Algal Membrane Protein, Heterosigma Akashiwo Na+–ATPase
    Taeho Jo, Mariko Shono, Masato Wada, Sayaka Ito, Junko Nomoto, Yukichi ...
    2010 年 35 巻 2 号 p. 80-85
    発行日: 2010年
    公開日: 2015/06/14
    DOIhttps://doi.org/10.5360/membrane.35.80
    ジャーナル オープンアクセス
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    The three–dimensional structure of Heterosigma akashiwo Na+–ATPase (HANA) was predicted by means of homology modeling based on the crystal structure of the K+–bound form of shark Na+/K+–ATPase (PDB ID: 2ZXE). The overall structure of HANA appears to be similar to that of shark Na+/K+–ATPase. Both contain three characteristic cytoplasmic domains, A, N and P, which are unique to P–type ATPases. HANA has a long TM7–8 junction as a large extracellular domain, in place of the β–subunit of shark Na+/K+–ATPase. Two putative K+–binding sites in the transmembrane domain of HANA were identified by means of valence mapping based on the constructed structure. The presence of K+–binding sites and the reported ion requirements for ATPase activity and EP formation indicate that HANA may transport K+ ions in the same manner as animal Na+/K+–ATPases.
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  • 細孔表面に固定したカルボキシベタイン基による 多孔性膜へのタンパク質の吸着の抑制
    松野 伸哉, 岩撫 暁生, 梅野 太輔, 斎藤 恭一, 伊藤 一, 坂本 雅司
    2010 年 35 巻 2 号 p. 86-92
    発行日: 2010年
    公開日: 2015/06/14
    DOIhttps://doi.org/10.5360/membrane.35.86
    ジャーナル オープンアクセス
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    N, N–dimethyl–γ–aminobutyric acid (DMGABA) as a carboxybetaine was introduced into the polymer chain grafted onto the pore surface of a polyethylene-made porous hollow-fiber membrane. The DMGABA–immobilized porous hollow-fiber membrane (DMGABA fiber) was prepared by the radiation–induced graft polymerization of glycidyl methacrylate (GMA) and the subsequent addition of DMGABA to the epoxy group of the grafted poly–GMA chain. The dose of the electron beam, the degree of GMA grafting (dg), and the molar conversion of the epoxy group into the DMGABA group were optimized to minimize the amount of protein adsorbed. As a result, a dose of 200 kGy, a dg of 140%, and a molar conversion above 3% were selected from the viewpoints of the protein binding capacity of the DMGABA fiber. The binding capacity of the DMGABA fiber for lysozyme in carbonate buffer (pH 9.0) was reduced to less than 10% that of the original polyethylene membrane. The lysozyme binding capacity of the DMGABA fiber in Britton–Robinson universal buffer (pH 7.0) was 17 ng per cm2 of the pore surface.
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