巻号一覧
9 巻 (1984)
- 6 号 p. 308-
- 5 号 p. 246-
- 4 号 p. 184-
- 3 号 p. 126-
- 2 号 p. 62-
- 1 号 p. 3-
9 巻, 6 号
選択された号の論文の7件中1～7を表示しています
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飯塚 哲太郎, 三谷 芙美子, 石村 巽1984 年 9 巻 6 号 p. 308-318
発行日: 1984/11/01
公開日: 2011/03/04
ジャーナル フリーFlash photolysis technique is one of the dynamical methods to determine kinetic parameters or to assign the reaction intermediate with short half life. Compared with other tachniques such as stopped flow and temperature jump methods, it has advantages in probing non-invasively the photosensitive pigments in tissues, cells, organella, and so on. This technique has been applied to studies on photosynthesis, visual pigments, hemoproteins, and etc. In this article we reviewed the application of this method to the analyses of membrane-bound hemoproteins such as cytochrome oxidase and cytochrome P450.抄録全体を表示PDF形式でダウンロード (1537K) -
吉澤 透, 小栗栖 太郎1984 年 9 巻 6 号 p. 319-328
発行日: 1984/11/01
公開日: 2011/03/04
ジャーナル フリーSpectroscopic, photochemical and biochemical properties of halorhodopsin are summarized. Halorhodopsin is present in the plasma membrane of Halobacterium halobium and acts as a light-driven Cl--pump. Halorhodopsin has an all-trans retinal as its chromophore which binds to apoprotein, halo-opsin through a protonated Schiff base. The absorption spectrum of halorhodopsin and its photoreaction depend on the chloride concentration in the medium. The λmax of halorhodopsin in the presence and absence of NaCl are 576 and 567 nm, respectively. The Cl--dependency of the absorption spectrum suggests the existence of two sites for binding Cl-; one is a “Cl--binding site” specific for Cl- and the other is an “anionbinding site” non-specific for Cl-. The Cl--binding site is responsible for the wavelength regulation of the absorption spectrum, while the anion-binding site is not. On the basis of these two binding sites, a light-driven Cl--pump mechanism has been suggested as follows : During the photoreaction of halorhodopsin, a Cl- is released from the Cl--binding site to the inside of the cell, then another Cl- in the anionbinding site shifts to the Cl--binding site and finally a Cl- in the medium outside of the cell is bound in the anion-binding site. Thus one Cl- in the medium can be transported into the inside of the cell by the aid of the photoreaction of one halorhodopsin molecule.抄録全体を表示PDF形式でダウンロード (1321K) -
米山 宏1984 年 9 巻 6 号 p. 329-340
発行日: 1984/11/01
公開日: 2011/03/04
ジャーナル フリーThe effects of coating of semiconductor photoelectrodes with thin conductive films have recently been investigated with the purpose of electrode stabilization in aqueous solutions. This article summarizes recent research activities in this area. The materials used in such coating are classified into three groups; noble metals such as Pt and Au, semiconducting oxides such as SnO2 and TiO2, and polymers such as polypyrrole and polymerized electrcactive reagents. The functions of the coated films and their roles in photoelectrochemical properties of semiconductors are discussed for the most simple case of metal film coating, in reference to metal/semiconductor Shottoky barrier photovolatic cells. Characteristic features of the film-coated electrodes are described for the above-mentioned three kinds of the coated films.抄録全体を表示PDF形式でダウンロード (1604K) -
相澤 益男1984 年 9 巻 6 号 p. 341-348
発行日: 1984/11/01
公開日: 2011/03/04
ジャーナル フリーMembrane materials are designed for molecular recognition, which are exemplified by glass thin membranes, macrocyclic compound liquid membranes, and biomolecule immobilized membranes. Uphill transport of glucose is demonstrated with a multi enzyme membrane. A novel enzyme membrane is synthesized to simulate the glucose responsive insulin release of pancrea. A lectin membrane is applied to make a biosensor for peroxidase. A bioaffinity sensor is made using the difference in affinity between ligand and analogue compounds to binding proteins. A pre-synaptic membrane model is synthesized to release a neurotransmitter on electrically stimulated call.抄録全体を表示PDF形式でダウンロード (934K) -
滝沢 章, 木下 隆利, 辻田 義治, 伊藤 貞典1984 年 9 巻 6 号 p. 349-350
発行日: 1984/11/01
公開日: 2011/03/04
ジャーナル フリーPDF形式でダウンロード (261K) -
関根 克尚, 花井 哲也, 小泉 直一1984 年 9 巻 6 号 p. 351-355
発行日: 1984/11/01
公開日: 2011/03/04
ジャーナル フリーDielectric observations were carried out on poly (methyl methacrylate) films immersed in aqueous KCl solutions of different concentrations. Relative permittivity and electrical conductivity of the film and those of the KCl solution phase were evaluated on the basis of a theory of interfacial polarization for a series combination model composed of a film and a KCl solution phase. Frequency dependence of the permittivity and the conductivity of the underwater film was approximated by a dielectric relaxation of the m-th power type. The permittivity and the conductivity of the underwater film were larger than those of a dry film measured in air. The permittivity of the underwater film was unaffected by the KCl concentration in the solution phase. The dc conductivity of the underwater films varied in proportion to the conductivity of the solution phase, being smaller than the conductivity of the solution phase by a factor of 2 × 10-5.抄録全体を表示PDF形式でダウンロード (543K) -
山下 京子, 堀内 道夫, 若林 隆, 桃田 道彦1984 年 9 巻 6 号 p. 357-364
発行日: 1984/11/01
公開日: 2011/03/04
ジャーナル フリーMitochondria in hepatocytes obtained from rats placed on a 1% hydrazine-diet for 7 days became gigantic, while those of rats treated with hydrazine for 3 days were slightly enlarged and sometimes elongated. Differential scanning calorimetry revealed that thermotropic phase transitions of liver mitochondria and the inner and outer membrane fractions obtained from hydrazine-treated rats for 3 days shifted to higher temperatures. Changes were most distinct in the outer membrane fractions with the increment in the enthalpy values. Increase in the ratio of acidic phospholipids, especially of phosphatidylserine, to the total phospholipids in the outer membranes and that in the amount of Ca2+ in mitochondria were also detected in hydrazine-fed rats for 3 days. All the changes, described above, were far less distinct in hydrazine-fed rats for 7 days suggesting that mitochohdrial membranes became more fluid by hydrazinetreatment and that once megamitochondria were formed membranes were stabilized.抄録全体を表示PDF形式でダウンロード (2018K)
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