衛生動物
Online ISSN : 2185-5609
Print ISSN : 0424-7086
ISSN-L : 0424-7086
33 巻, 1 号
選択された号の論文の16件中1~16を表示しています
  • 原稿種別: 表紙
    1982 年 33 巻 1 号 p. Cover2-
    発行日: 1982/03/15
    公開日: 2016/09/03
    ジャーナル フリー
  • 下村 浩, 石井 明, 高岡 正敏, 加納 六郎
    原稿種別: 本文
    1982 年 33 巻 1 号 p. 1-7
    発行日: 1982/03/15
    公開日: 2016/09/03
    ジャーナル フリー
    An experiment was carried out to isolate each stage of house dust mites [Dermatophagoides farinae Hughes, 1961,and D. pteronyssinus (Trouessart, 1897)] from mass culture with sieves of different mesh sizes, and further, to separate eggs from culture medium with a saccharose sedimentation technique. The fluctuation in number and age composition of a cultured population originated from approximately 1,000 eggs, which were obtained with the present method, was traced for 6 months. Culture medium (100g) was composed of equal amounts of dried yeast and mouse food powder. 1. The mite was separated roughly into each stage, and egg stage was purely collected by sieving. 2. More than 94% recovery was obtained at a saccharose concentration of 30% for D. farinae and 40% for D. pteronyssinus with adequate separation from culture medium. 3. The number of live mite increased up to (10)^4 mites at the 3rd month after beginning of the culture and began to decrease at the 5.5th month. On the other hand, the number of dead mites continued to increase gradually, and reached the same number to that of live mites at the 4th month and the 5th month in D. farinae and D. pteronyssinus, respectively. 4. The population size reached the peak at the 5th, 3rd, 4th and the 3.5th month for the female, male, trito-and protonymphs and for the larva, respectively, in a culture of D. farinae.
  • 藤崎 幸蔵, 北岡 茂男
    原稿種別: 本文
    1982 年 33 巻 1 号 p. 9-15
    発行日: 1982/03/15
    公開日: 2016/09/03
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    The tick, Haemaphysalis longicornis (parthenogenetic Okayama strain), was cultured for two generations using wholly the rabbits (IR) aquired resistance to the ticks by successive infestations of females. The ticks (IT) fed on IR was significantly lighter in weight at all the developmental states observed except unfed larvae than ticks (NT) fed on nonimmunized rabbits (NR). The precipitating antibody titer rose in all the IR after larval or nymphal infestations. The resistance of rabbits to female H. longicornis appeared equally to the immature stages of this tick, and the immuno-diffusional antigens were found to be common with larva, nymph and adult. No significant differences were observed between the weight and size of egg and fresh larva of IT and NT. This showed that some biological characters of the egg and larva were keeping more rigid innate characteristics not affected by immunological state of hosts comparing to the subsequent developmental stages. The IT females engorged on NR were significantly lighter than NT females engorged on NR, which evidence might be interpreted as the long term effects of IR on ticks. Although the total amount of excreta discharged by IT female was less than 10% of that by NT female, no significant differences were observed between content of nitrogen or iron in excreta of IT and NT. This evidence suggested that the activity of digestion and absorption in IT might be normal.
  • 山西 浩
    原稿種別: 本文
    1982 年 33 巻 1 号 p. 17-20
    発行日: 1982/03/15
    公開日: 2016/09/03
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    Reaction of 50 female mosquitoes to mouse wrapped cylindrically with filter paper in a cage was observed. The mosquitoes showing probing behavior were counted at an interval of 3min. More than 20 individuals showed probing behavior within 15min and the number decreased thereafter. Few individuals reacted after 45min to the filter. Using this device, diurnal and daily changes of the probing behavior after emergence and difference of the behavior between Aedes albopictus and Aedes aegypti were investigated.
  • 武衛 和雄, 伊藤 寿美代
    原稿種別: 本文
    1982 年 33 巻 1 号 p. 21-25
    発行日: 1982/03/15
    公開日: 2016/09/03
    ジャーナル フリー
    日本脳炎ウイルスをとりこんだコガタアカイエカが伝播可能となるまで, どのくらいの割合で生存しているかを推定することは, その地域での伝播の度合を知るうえに必要なことである。大阪府下のある地域で採集したコガタアカイエカ個体群について, 1968∿1973年の6年間, 年令構成の季節的, 年次的に変動する様相を観察した。その結果, 未経産蚊率の変動は蚊の発生数の変化によって生ずること, 8月の平均経産率(18.1%)は7月のそれ(15.4%)に比べてわずかに高いことを示した。さきに報告したごとく, 本蚊の野外におけるgonotrophic cycleは2.9日であったので, これらから日あたりの生存率が推定された。すなわち, 7月は0.525,8月は0.555であった。これらの値の年による変動はさほど大きくはないように思われる。
  • 岡本 雅子, 松本 克彦, 和田 芳武, 中野 寿夫
    原稿種別: 本文
    1982 年 33 巻 1 号 p. 27-31
    発行日: 1982/03/15
    公開日: 2016/09/03
    ジャーナル フリー
    In this study we investigated the antifungal effect of gaseous citral on various fungi by "the modified gas method". The 22 species of fungi were used in this experiment (Table 1). Many of them are often observed in these stored products and some of them are pathogenic to men and animals. The concentrations of citral hexane solution were 1,5,10 and 20mg/ml and 0.05ml of them was impregnated in a paper disc. The cultures were incubated at 28℃ and the radial growth was measured every 24hr for 4 or 5 days. The growth inhibitory rate of citral to various fungi was calculated from the square measure of the colony after 72hr of incubation and was shown in Fig. 2. In Trichoderma sp. the inhibitory activity of citral at each concentration was observed after 48hr incubation, but after 72hr only 20mg/ml of citral hexane solution inhibited the radial growth of fungi compared with that of control. And in Rhizopus nigricans, which grew most rapidly in the 22 species of fungi, the antifungal effect of citral was observed after 24hr incubation, but it could not be observed at all after 48hr. Because the mycelium of that fungi expanded too quickly and covered all the medium even with 20mg/ml of citral. Though the antifungal activity of citral is different by the species of the fungi, it is obvious that the gaseous citral has antifungal effect on all the 22 different species of fungi investigated. So it is supposed that, in the environment of the grain mites, citral released by the mite inhibits the growth of fungi and is indispensable to the survival of mites.
  • 田口 五弘, 真喜 屋清
    原稿種別: 本文
    1982 年 33 巻 1 号 p. 33-39
    発行日: 1982/03/15
    公開日: 2016/09/03
    ジャーナル フリー
    ゲジThereuonema hilgendorfi Verhoeffが蚊の捕食者としてどの程度天敵の役割を果たしているかを知るために, いくつかの室内実験を行って, その活動時間およびいろいろな密度条件における捕食量を推定すると同時に, 野外で採集したものについて胃の内容物を調べた。すす紙についた足跡の数から見て, ゲジは夜間, とくに前半夜に活発に動きまわることがわかった。ゲジ(捕食者)にアカイエカCulex pipiens pallensを餌として与えた場合, 捕食される蚊の数(n)は経過日数(t)の指数関数n=N[1-exp(-a・t)]として表され, 餌密度(N)と捕食効率(a)によって捕食蚊数が変化することがわかった。捕食者と餌の密度をいろいろと変えて実験を行った結果, 捕食される蚊の数は捕食者の密度が同じ場合は餌密度が高いときに, また餌密度が同じならば捕食者密度が高いときに, それぞれ多いものと考えられ, 捕食蚊数は平均温度17.5∿25.0℃下でゲジ1匹1日あたり0.3∿1.3匹と推定された。11月に越冬アカイエカとゲジが棲息する防空壕からゲジを採集し胃の内容物を調べたところ, ほとんどのゲジから蚊の断片が見つかり, 平均2匹ほどのアカイエカが1匹のゲジによって捕食されていることがわかった。
  • 池庄司 敏明
    原稿種別: 本文
    1982 年 33 巻 1 号 p. 41-49
    発行日: 1982/03/15
    公開日: 2016/09/03
    ジャーナル フリー
    metepaを処理したビーカーにスピーカーをセットし, 蚊の羽音を混合したり, 周波数, 強度を変調させ, 雄蚊に対する誘引性を, 卵のふ化率の低下で調べた。雌蚊の周波数なら単一の純音で充分に誘引した。音の強度の変調では, Ae. aegyptiに対して, 466Hz, 98dB SPLの音が, またCx. p. molestusに対しては, 370Hz, 124dBの音が最も卵のふ化率を低下させた。さらに466Hz, 124dBの純音は, 100mの遠距離まで到達し, Ae. aegyptiを不妊化させ得ると推論した。卵のふ化率は, 不妊剤の処理薬量に反比例し, Ae. aegyptiでは660μg/(cm)^2で0%, 20μg/(cm)^2で9.7%であった。以上, 自動不妊化法は雄蚊の大量誘殺より, はるかに優れた蚊の駆除法になることを示した。
  • 上村 清, 渡辺 護
    原稿種別: 本文
    1982 年 33 巻 1 号 p. 51-54
    発行日: 1982/03/15
    公開日: 2016/09/03
    ジャーナル フリー
    The distribution patterns of larvae of Anopheles sinensis and Culex orientalis were studied in a paddy field in Toyama Prefecture. The numbers of mosquito larvae were counted, by 6 different experimenters, from 12 sections (6 peripheral and 6 inner) in the paddy field, scooping 10 times each in every section. The analysis of variance revealed that, within any one section, little difference was observed in larval counts between the 2 species as well as among different experimenters. The average number of larvae of both species was smaller in 3 peripheral sections where water was flowing, in comparison to the other 3 peripheral or 6 inner sections where water was still. Younger larvae were found more abundantly in the peripheral sections than in the inner ones, while older larvae were abundant in the latter sections.
  • 浦辺 研一, 関島 安隆, 池本 孝哉, 会田 忠次郎
    原稿種別: 本文
    1982 年 33 巻 1 号 p. 55-60
    発行日: 1982/03/15
    公開日: 2016/09/03
    ジャーナル フリー
    The precipitin test using the electrosyneresis (ES method) was evaluated for the purpose of detecting the mosquito larvae (An. sinensis) from the gut of the dragonfly nymphs (Sympetrum frequens), in the course of studies for their prey-predator relationships in rice fields. Antiserum was obtained from rabbits immunized by intramuscular injections of saline extracts of An. sinensis (4th instar larvae). This antiserum showed the strong cross-reaction between An. sinensis and Cx. tritaeniorhynchus, and Chironomus sp. which might be fed by the dragonfly nymphs in the census fields. The specific antiserum to An. sinensis larvae was prepared by absorbing the antiserum with the saline extracts of Cx. tritaeniorhynchus. Using the specific antiserum on ES method, it was experimentally confirmed that the 4th instar larva of An. sinensis in the gut of the 9th instar nymphs of Sympetrum frequens could be detected with confidence up to 33hr after feeding in all cases of trials and up to 54hr in one case. It was concluded that the ES method could be useful and appliable to the quantitative study of their prey-predator relationships.
  • 江下 優樹, 栗原 毅, 緒方 隆幸, 大谷 明
    原稿種別: 本文
    1982 年 33 巻 1 号 p. 61-64
    発行日: 1982/03/15
    公開日: 2016/09/03
    ジャーナル フリー
    Females of 15 species of Japanese mosquitoes were studied to determine if they were susceptible to mouse-adapted dengue-2 virus (New Guinea C strain) by oral administration of the virus mixed with rabbit blood and sucrose. The following species were tested : Aedes (Stegomyia) albopictus, Ae. (Stg.) flavopictus, Ae. (Stg.) riversi, Ae. (Ochlerotatus) dorsalis, Ae. (Aedimorphus) vexans nipponii, Ae. (Finlaya) japonicus, Ae. (Fin.) togoi, Armigeres (Armigeres) subalbatus, Anopheles (Anopheles) sinensis, Culex (Culex) orientalis, Cx. (Cux.) pipiens molestus, Cx. (Cux.) pipiens pallens, Cx. (Cux.) pipiens quinquefasciatus, Cx. (Cux.) tritaeniorhynchus and Tripteroides (Tripteroides) bambusa. Several hundred mosquitoes were used for each species. They were kept at 30℃ after the infection, and were stored at -80℃ after the incubation of various time length from 0 to 30 days. The detection and titration of the virus were made by the intracranial injection of a suspension pool of 10 mosquitoes in suckling mice (ddY strain). The virus was detected at varying titers from all the mosquito species immediately after the infection, but was detected after 20 days of infection only from the four species, i. e., Ae. (Stg.) albopictus, Ae. (Stg.) flavopictus, Ae. (Stg.) riversi and Ae. (Och.) dorsalis, ranging from "trace" to≧(10)^<4.6> LD_<50> per pool. In more detailed studies with the positive mosquito species, the virus was found to multiply after an eclipse period of 7 to 10 days and to reach a peak titer after 20 to 30 days.
  • 江下 優樹, 栗原 毅, 緒方 隆幸, 大谷 明
    原稿種別: 本文
    1982 年 33 巻 1 号 p. 65-70
    発行日: 1982/03/15
    公開日: 2016/09/03
    ジャーナル フリー
    The purpose of this study was to examine methods of improving the mass rearing techniques of a non-biting mosquito Toxorhynchites amboinensis (Doleschall), which is an efficient laboratory host for dengue virus when the virus is injected intrathoracically. Experiments were directed at (1) the establishment of individual rearing techniques thus preventing larvae cannibalism; (2) the development of a suitable food for the larvae; and (3) the definition of the optimum physical conditions for larvae growth. 1. The larvae were reared individually in 3ml disposable trays containing 2ml of water or in 200ml plastic cups containing 50ml of water with Aedes albopictus (Skuse) larvae provided as food. The larval period at 27℃ was 18.1 days with a pupation rate of 89.1% in the disposable trays and 18.6 days and 94.1% in the plastic cups. In group rearing of 50 larvae in plastic trays (27×35×8cm) containing 3 liters of water, the larval period was 18.1 days and the pupation rate was 29.9%. It was shown that the individual method gives higher pupation rates and that the use of larger containers give slightly better results. 2. The larvae were reared using the following feed : Tetramine^[○!R], Vita-shrimp^[○!R], liver powder, dried water fleas, dried blood worms, and drone powder. The average larval periods were 47.7,45.5,56.0,53.7,43.7 and 52.0 days, respectively. The pupation rates were 70,70,50,80,40 and 10%, respectively. Larvae reared with a combination of a living diet of newly hatched brine shrimp Artemia salina (Linnaeus) for the young larval stage and the blood worm Limnodrilus hoffmeisteri Claparede for the third and fourth larval stages pupated after an average of 23.2 days at 25℃ with a pupation rate of 100%. These results were almost the same or slightly better than those obtained with group rearing by the conventional method providing Aedes albopictus larvae in which the larval period was 24.1 days and the pupation rate 90%. 3. The larvae were reared by providing a combined living diet at the temperature levels of 20,25,27 and 30℃. No larval growth was seen at 20℃, and the average preadult periods and pupation rates at the higher temperatures were 32.2,29.4 and 27.4 days, and 100,90 and 80%, respectively. At 30℃ the death rate of the blood worms was high enough to cause fouling of the water.
  • 高岡 宏行
    原稿種別: 本文
    1982 年 33 巻 1 号 p. 71-72
    発行日: 1982/03/15
    公開日: 2016/09/03
    ジャーナル フリー
    1980年9,10,11月および1981年の7月に, 大分県の祖母山山系を流れる奥岳川の岸床凹みの水たまりに棲息するヤマトマブカAedes japonicusの幼虫集団に未記録のmicrosporidanによる感染が見いだされたので本邦産蚊類における初めての例として報告した。
  • 早川 博文, 米山 陽太郎
    原稿種別: 本文
    1982 年 33 巻 1 号 p. 73-75
    発行日: 1982/03/15
    公開日: 2016/09/03
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    Egg-batches of Chrysops japonicus Wiedemann, 1828 were collected in a paddy field at Nishine, Iwate-ken during from late June to middle July in 1980. The eggs were laid on the stems of Scirpus triqueter L. and on the undersides of leaves of 8 other plants (Phragmites communis Trinius was most frequently laid on) at the average height of 31cm above the surface of the water. The egg-batch was greyish yellow to dark brown in color with darker tints on the upper end of each egg, and a little swollen in shape with 2 to 3 layers of eggs. Average number of eggs per batch was 373.
  • 江下 優樹
    原稿種別: 本文
    1982 年 33 巻 1 号 p. 77-94
    発行日: 1982/03/15
    公開日: 2016/09/03
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  • 原稿種別: 表紙
    1982 年 33 巻 1 号 p. Cover4-
    発行日: 1982/03/15
    公開日: 2016/09/03
    ジャーナル フリー
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