It is necessary to establish a standard method in evaluating an immature simuliid density. This paper describes the results of the comparison of various artificial substrates (i. e., bricks, polyvinyl chloride (PVC) sheets, PVC or silicone tubes) with unit time (10min) collection in the fields in Japan and Guatemala. In Tanzawa, Kanagawa Prefecture, Japan, PVC sheets and normal bricks were compared with unit time collection monthly. Mean numbers of immature simuliids captured by bricks, PVC sheets and unit time collection were 3.6,33.8 and 65.8,respectively (Table 1). Thus, bricks were not recommendable as the artificial substrate. The seasonal occurrence was observed by PVC sheets. Simulium japonicum were dominant (96%) and occurred throughout the year. S. suzuki and S. bidentatum occurred during May to September abundantly, while S. subcostatum January to March. Prosimulium hirtipes group was shown March to April (Table 2). In Mt. Tsukuba, Ibaraki Prefecture, Japan, PVC sheets and tubes were compared with unit time collection monthly. Total number of S. japonicum, dominant species (69%) in the tributary No. 8 of the Yamaguchi River, captured by PVC tubes, sheets and unit time collection were 1,507,875 and 669,respectively (Table 4). In 4 stations from the headwaters to the main stream, the species composition and its seasonal change were observed. S. subcostatum was abundant from the head to the middle reaches; S. japonicum was so at the middle courses where the water runs rapidly; and S. aokii was found in the lower courses of the tributary and the main stream also (Table 3). Each species showed a specific occurrence pattern (Figs. 4-7). In the River Los Lavaderos and the River Guachipilin in the pilot area for research and control project of onchocerciasis, Municipality of San Vicente Pacaya, Department of Escuintla, Guatemala, the silicone tubes were compared with unit time collection fortnightly. In the River Los Lavaderos, the number of larvae and that of species were not different widely between both methods (Table 5), while in the River Guachipilin, unit time collection varied widely according to the skill of collectors (Table 6,Fig. 8). As a conclusion, the author recommends that the use of unit time collection together with the artificial substrates (PVC sheets and/or silicone tubes according to the condition of the stream) is preferable for the evaluation of the immature simuliid density.
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