This is the first record of Sergentomyia squamirostris in Tokyo, Japan. Sand flies were collected from the western part of Tokyo, in August and September 2016, one male and seven females from Nishitama District and two females from Hachioji city. Morphological examination of the cibarium, pharynx, and spermathecae in female, and the genitalia, coxite and style in male revealed that all these specimens belonged to S. squamirostris.