Major Histocompatibility Complex
Online ISSN : 2187-4239
Print ISSN : 2186-9995
ISSN-L : 2186-9995
Volume 24, Issue 1
Displaying 1-3 of 3 articles from this issue
Review
  • Kimiaki Ogawa
    2017 Volume 24 Issue 1 Pages 38-45
    Published: 2017
    Released on J-STAGE: April 28, 2017
    JOURNAL FREE ACCESS

    There are two laboratory testing methods for HLA. One is so-called HLA typing that had been previously done by serological detection of HLA antigens expressed on the cell surface of lymphocytes by using anti-sera produced against non-self HLA antigen, and has now been done as so-called HLA-DNA typing. The other HLA testing method is to detect the anti-HLA-antisera generated against non-self HLA antigens found in sensitized subjects. There are several different laboratory procedures for HLA-DNA typing and detection of anti-HLA-antisera, for which commercially available kits have been developed. In this review, principle and management of procedures for HLA testing including requirement of check-points at the timing of lot change for commercial kits.

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  • Yuko Ohnuki, Shigeaki Suzuki, Atsuko Shigenari, Shingo Suzuki, Norihir ...
    2017 Volume 24 Issue 1 Pages 46-53
    Published: 2017
    Released on J-STAGE: April 28, 2017
    JOURNAL FREE ACCESS

    Immune-mediated necrotizing myopathy (IMNM) is a relatively newly recognized subgroup of idiopathic inflammatory myopathies. Although it has similar symptoms to polymyositis clinically, IMNM is distinguished from the other inflammatory myopathies by the absence of prominent inflammatory infiltrates histologically. IMNM has been known to be associated with myositis-specific autoantibodies such as anti-SRP and anti-HMGCR antibodies. It also may be associated with statin, malignancy and connective tissue diseases. This review provides an overview of IMNM and describes our study analyzed alleles of HLA-A, B, C, DRB1 in IMNM patients.

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  • Mihoko Shimada-Sugimoto, Katsushi Tokunaga
    2017 Volume 24 Issue 1 Pages 54-64
    Published: 2017
    Released on J-STAGE: April 28, 2017
    JOURNAL FREE ACCESS

    Panic disorder (PD) is an anxiety disorder characterized by panic attacks and anticipatory anxiety. To date, few genetic and environmental factors were found to be involved in PD and pathogenesis of PD is remained to be elucidated. Single nucleotide polymorphisms (SNPs) in TMEM132D and COMT, are only a few genetic factors of PD that were replicated in several studies in European population, but not in Japanese population. We previously performed a genome-wide association study (GWAS), however, there seemed to be polymorphisms which did not reach genome-wide significance threshold due to their low allele frequencies and odds ratios, although they were truly associated with PD. We then performed pathway analyses to overcome the limitations of a conventional single-marker analysis. The pathway analyses identified the associations of immune pathways with PD. Based on the results of pathway analyses, we especially focused on and investigated HLA-B and HLA-DRB1. As a result, a frequency of HLA-DRB1*13:02 was significantly higher in PD patients than in control (P=2.62×10−5, odds ratio=1.70). We further examined sub-group analyses of GWAS, taking effects of HLA alleles into account. The SNP genotype data were subdivided into two datasets: those of HLA-DRB1*13:02-positive subjects (cases: N=103; controls: N=198) and those of HLA-DRB1*13:02-negative subjects (cases: N=438; controls: N=1,341). As a result, one SNP in MCPH1 showed a genome-wide significant association with PD and several SNPs in TMEM132D showed suggestive associations with PD in subjects without HLA-DRB1*13:02.

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