DNA typing methods based on the polymerase chain reaction(PCR)technology have been rapidly developed to determine the HLA alleles, and these procedures have been spread in place of serological method. Any PCR-based typing methods require a few additional procedures after the PCR amplification. We developed a new DNA typing method(TaqMan PCR-SSP)that can directly determine the HLA-DRB alleles using the PCR-SSP technique with dual-labeled fluorogenic probes called as TaqMan probe, without any complicated operations after the amplification. Two TaqMan probes and a commercially available primer kit were used in this typing system. Twenty-nine samples in which the HLA alleles had already been proven were tested in this study. The typing results were correct, and the experimental results were clear-cut and neither met false positive nor false negative reaction. The TaqMan PCR-SSP method may be easily applicable to HLA typing as a routine test in clinical laboratory, because there were no complicated and additional operations, so that artificial mistakes and experimental time could reduced.