“NARINE” is lyophilizated biomass of live cultures Lactobacillus acidophilus Er-2 Strain 317/402 and “Narine” strain has the difference of advantages from those previously known as follows; it possesses clearly expressed adhesive properties, possesses high adaptability and stays long in the intestine (two weeks), possesses high bile-and acid-resistance (PH=3.0), possesses high antibiotic resistance, and possesses high antibacterial, antibiotic and antimycotic activities. Therefore “NARINE” has many useful characters for human as fellows; it is regulator of the intestine microbial biocenosis, it is immunomodulator it stimulates synthesis of vitamins and lactic acid in the organism, it ensures assimilation of calcium, phosphorus, vitamins, proteins, carbohydrates, it neutralizes toxic products of metabolism, it is breast milk substitute, amd acido-lactic bacteria are characterized by their resistance to the action of antibiotics and other chemotherapeutic drugs, as well as by high adaptability in the gastroenteric tract.
 Effects of “Narine” on the clinical course of many fields such that dysbacteriosis of the intestine, the oncologic practice, antibiotic therapy, postnatal mastitis, prophylaxis of infectious diseases of staphylococcic etiology, obstetrics, gynecology, radiation-thermal injury, hypercholesterolemia, Periodic disease, gastroesophagial reflux, chronic pancreatitis, treatment of Helicobacter pylori-associated pathologies, cosmetology, parodontosis has been studied during more than 40 years in Armenia and Russian. We report several parts of their academic achievements by review.

View full abstract

 Micellar calcium phosphate (MCP) was prepared as a complex with phosphopeptide (CPP) from rennet casein using the following procedures. Rennet casein was suspended in water, hydrolyzed by trypsin, and the resulting suspension was heated to stop the enzymatic reaction. The pH was adjusted to 4.6, and the suspension was centrifuged. The supernatant was adjusted to pH 9.0, concentrated by ultrafiltration with a membrane (cut off 30 kDa), and then lyophilized. The dried MCP-CPP complex was easily dissolved in water and contained 63.1% peptide, 9.2% calcium, 3.5% inorganic phosphorus and 1.8% organic phosphorus based on the dry basis. The X-ray diffraction pattern revealed the absence of hydroxyapatite in the MCP-CPP complex. It was confirmed by gel permeation HPLC that MCP cross-linked CPP in the solution of the MCP-CPP complex as that in the casein micelles. Although ion exchange HPLC showed that the prepared MCP-CPP complex contained peptides other than CPP, almost no bitter taste was detectable. Five kilograms of rennet casein was suspended in 80 L of water and then treated by a method similar to the one above, to prepare 1.12 kg of the MCP-CPP complex. Although the MCP-CPP complex prepared in the present study contained somewhat less calcium and CPP than those in the previous one, it was prepared without the use of ethanol, suggesting that it can be prepared at a lower cost.

View full abstract

 To elucidate the effect of yogurt containing Bifidobacterium lactis BB-12 (4×10⁹ cfu/100 g, BB-12 yogurt) on the defecation for intestinal microflora,a placebo control cross-over trial was conducted. Twenty nine female adults (average age 22.0 years) were divided into two groups, and either of BB-12 yogurt or placebo yogurt without Bifidobacterium lactis BB-12 was administered at 100 g/day for 14 days. The defecation frequency (days) obviously increased during BB-12 yogurt period compared to placebo yogurt period in the subjects with mild constipation. The fecal microflora of 20 adults selected from 29 adults were studied. Increase of the number of Bifidobacterium and ratio of Bifidobacterium to total bacteria, decrease of the number of Bacteroidaceae and ratio of Bacteroidaceae to total bacteria, decrease of ratio of Enterobacteriaceae to total bacteria, improvement of fecal shape were obviously observed during BB-12 yogurt period compared to placebo yogurt period in the subjects with mild constipation. These results suggest that the yogurt containing Bifidobacterium lactis BB-12 shows to improve the defecation, intestinal microflora and intestinal environment.

View full abstract