Bifidobacterium lactis BB-12 株（BB-12）を 4×10⁷ cfu/g 含有するヨーグルト（BB-12 ヨーグルト）の便性および糞便内細菌叢の改善効果に対する BB-12 ヨーグルト摂取量の影響を検討した。健常成人15名（平均年齢39.8歳）を 2 群に分け，BB-12 ヨーグルトを 1 日80 g または150 g を14日間摂取させた。150 g 摂取群では排便量の増加が認められ，一方80 g 摂取群および150 g 摂取群では，糞便内細菌叢改善および糞便アンモニア含量の低下が見られた。これらから，1 日80 g から150 g の間では，整腸効果は摂取量依存的に誘導されると考えられた。
 また，過剰量摂取時の安全性を明らかにするため，1 日450 g で，14日間の摂取試験を実施した。試験期間中，異常所見は認められず，過剰量摂取時の安全性が確認された。

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 β-Lactoglobulin (β-LG) is a major allergen protein in cow's milk. The antigenic activity in soluble fractions of bread supplemented with dried skim milk was investigated by ELISA competitive inhibition and immunoblotting analyses using a rabbit anti β-LG. The bread added the dried skim milk dosage to be 0.1, 0.5 and 1 for the flour weight was prepared. Almost no antigenic activity of β-LG was detected in the salt solution extracts of bread with the addition of 0.5 skim milk dosage or less. β-LG was detected in 1% SDS containing 10% 2-mercaptoethanol (2-ME) extract from the bread but not with SDS only. It is suggested that the decrease in antigenic activity of β-LG was based on heat-induced polymerization through intermolecular disulfide bonds among β-LG and wheat proteins. The bread was hydrolyzed with pepsin until 180 min. β-LG was also not detected in the supernatant of pepsin digest of the bread supplemented with 0.5 dosage skim milk, whereas the precipitate of the pepsin digest included insoluble β-LG. Thus, no detectable level of antigenic activity of β-LG was recovered in the soluble fractions of the pepsin digest of the bread added skim milk.

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