Members of the genus
Lactobacillus are good candidates for probiotics. Methods are needed to select for these microorganisms. In this study, we isolated lactobacilli using two selective media, MRS agar and modified LBS (mLBS) agar; and compared their selectivity for lactobacilli. The detection rate for lactobacilli was different comparing MRS agar and mLBS agar, 59.4% and 75.0%, respectively. A growth test showed
Lb. plantarum,
Lb. coryniformis,
Lb. rhamnosus,
Lb. gasseri,
Lb. brevis,
Lb. fermentum,
Lb. alimentarius,
Lb. paracasei and
Lb. casei strains grew on mLBS agar; whereas the growth of
Lb. sakei was slight and no growth for
Lb. curvatus and
Lb. rossiae. All strains isolated using mLBS agar could grow on MRS agar. The results suggest selectivity of mLBS agar was attributed to its acidity.
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