Milk Science
Online ISSN : 2188-0700
Print ISSN : 1343-0289
ISSN-L : 1343-0289
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Volume 60, Issue 3
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Original Paper
  • Comparison of selectivity of MRS agar and modified LBS agar for isolation of lactobacilli
    Hideki Kinoshita, Shun Imoto, Yoshihito Suda, Mitsuharu Ishida
    2011 Volume 60 Issue 3 Pages 171-176
    Published: 2011
    Released on J-STAGE: March 15, 2014
    DOIhttps://doi.org/10.11465/milk.60.171
    JOURNAL FREE ACCESS
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     Members of the genus Lactobacillus are good candidates for probiotics. Methods are needed to select for these microorganisms. In this study, we isolated lactobacilli using two selective media, MRS agar and modified LBS (mLBS) agar; and compared their selectivity for lactobacilli. The detection rate for lactobacilli was different comparing MRS agar and mLBS agar, 59.4% and 75.0%, respectively. A growth test showed Lb. plantarum, Lb. coryniformis, Lb. rhamnosus, Lb. gasseri, Lb. brevis, Lb. fermentum, Lb. alimentarius, Lb. paracasei and Lb. casei strains grew on mLBS agar; whereas the growth of Lb. sakei was slight and no growth for Lb. curvatus and Lb. rossiae. All strains isolated using mLBS agar could grow on MRS agar. The results suggest selectivity of mLBS agar was attributed to its acidity.
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