Interactions of AFB
1 and DHBV, both of which are known to be carcinogenic to the livers, were analyzed in vitro. Primary hepatocyte cultures were performed using a duckling infected with DHBV. AFB
1 was added continuously to the culture media at a concentration of 0.1 or 1.0 ng/ml, and the cells were cultured for a month. Spot hybridization of the culture media did not reveal any significant difference in the amount of DHBV-DNA between AFB1 administra-tion groups and control group. However, AFB
1 administration increased numbers of particles and antigen of DHBV within the cultured hepatocytes. It was suggested that AFB
1 gave some influence on replication and expression of DHBV.
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