巻号一覧
1998 巻 (1998)
- 47 号 p. 1-
- 46 号 p. 1-
1998 巻, 46 号
選択された号の論文の7件中1～7を表示しています
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佐藤 洋一, 鈴木 理之, 根本 清光, 杉浦 義紹, 上野 芳夫1998 年 1998 巻 46 号 p. 1-8
発行日: 1998/01/31
公開日: 2009/08/04
ジャーナル フリーA trial for chemotaxonomic analysis of toxigenic Fusarium species was performed by an introduction of restriction fragment length polymorphisms based on the selected DNA probes of Fusarium sp. Fn 2B strain, a well-known producer of trichothecene mycotoxins nivalenol and fusarenon-X. A total DNA of F. Fn 2B was prepared from the protoplast suspension of mycelia, digested with BamHI, and ligated into BamHI site of plasmid vector pUC19. Two clones harboring inserts in sizes of approximately 1.1 and 1.7 kb were randomly selected for probes. Hybridization patterns of 32P-labeled 1.1 and 1.7 kb fragments of Fn 2B with BamHI, EcoRI or HindIII-digested DNAs from F, sporotrichioides (4 isolates), F, graminearum (6 isolates), F, tricinctum (4 isolates), F. poae (4 isolates), F. crookwellense (3 isolates) and others, were compared by Southern blots. The data obtained with the 1.1 kb fragment have revealed a similar pattern of restriction fragment profiles among the isolates of same Fusarium species such as F. sporotrichioides, F, tricinctum or F. graminearum. While, the fragment profile of Fn 2B strain did not correspond to that of all the other Fusarium spp. tested. This suggested that F. sp. Fn 2B, originally reported as F. nivale by Dr. Tsunoda and later cited as F. tricinctum at NRRL by J. J. Ellis and to F. sporotrichioides at the Medical Research Council Collection (MRC, Tygerberg, South Africa) by Marasas et al., may be unidentified Fusarium species.抄録全体を表示PDF形式でダウンロード (3450K) -
伊藤 陽子, ステフェン・ダブリュ ピーターソン, 後藤 哲久1998 年 1998 巻 46 号 p. 9-15
発行日: 1998/01/31
公開日: 2009/08/04
ジャーナル フリーAspergillus nomius, an aflatoxigenic species in Aspergillus section Flavi was first reported by Kurtzman et al. in 1987, but this fungus in Japan has never been reported. Several aflatoxigenic fungi were isolated from Japanese tea-field soil and silkworm excrements that display characteristics consistent with A. nomius. In order to identify these isolates we examined their colony color, micromorphological characters, mycotoxin production and DNA sequences. These fungi have light olive to moss green colony color, the conidia are spherical to subspherical having very rough surface ornamentation and their diameter is about 4.6μm. They produce aflatoxins B1, B2, G1, and G2 but do not produce detectable amounts of cyclopiazonic acid. The DNA sequences from the ITS and large subunit rDNA are identical with the sequence of an ex type strain of A. nomius. On the basis of these data we determined that two of these isolates are A. nomius.抄録全体を表示PDF形式でダウンロード (728K) -
長嶋 等, 後藤 哲久1998 年 1998 巻 46 号 p. 17-22
発行日: 1998/01/31
公開日: 2009/08/04
ジャーナル フリーTo test the hypothesis that the toxicity of rubratoxin B is a consequence of apoptosis, we investigated fragmentation of nuclei and internucleosomal fragmentation (DNA ladder). Exposure to rubratoxin B caused HL-60 cells to condense their chromatin and fragment their nuclei, showing that rubratoxin B induces apoptosis. We observed DNA ladder in HL-60 cells treated with rubratoxin B for 24 hr. Cycloheximide did not affect rubratoxin B-induced DNA ladder. Therefore, rubratoxin B-induced apoptosis is considered not to require protein synthe-sis. Twenty four-hr treatment of rubratoxin B or ethylene glycol-bis (β-aminoethyl ether)-tetraacetic acid (EGTA) alone resulted in DNA ladder, but no DNA ladder treated with both chemicals for 24 hr. Eight-hr treatment of rubratoxin B and EGTA resulted in DNA ladder, suggesting that long exposure to both chemicals led to post-apoptotic DNA degradation. Since the addition of Ca2+ inhibited the effect of EGTA, the reduction of extracellular Ca2+ concentra-tion by EGTA appears to cause an enhancement of rubratoxin B-induced DNA degradation.抄録全体を表示PDF形式でダウンロード (4077K) -
深田 恒夫, 岡本 美奈子, 笹井 和美, 馬場 栄一郎1998 年 1998 巻 46 号 p. 23-28
発行日: 1998/01/31
公開日: 2009/08/04
ジャーナル フリーThe purpose of this study was to investigate the enhancement of Salmonella typhimurium colonization and changes in the intestinal tract in chickens administered with ochratoxin A. Eleven-day old chickens received 108 colony-forming units S. typhimurium orally for 2 consecutive days. The 13-old-day chickens were administered a dose of ochratoxin A (2.0mg/kg/bird) orally. The number of S. typhimurium in both the duodenal and cecal contents of chickens administered with this dose of ochratoxin A increased significantly when compared with control birds. There were no significant differences in chickens administered doses of ochratoxin A below this level. There were significant differences in pH value, oxidation-reduction potential, and amount of acetic acid in duodenal and cecal contents between ochratoxin A-administered chickens and control ones. Number of feces were counted and decreased in chickens administered with ochratoxin A. Feed intake decreased by administration of ochratoxin A. Experi-mental evidence indicated that S. typhimurium colonization enhanced in the duodena and ceca of ochratoxin A-administered chickens and that fecal counts of chickens administrated with the ochratoxin A decreased.抄録全体を表示PDF形式でダウンロード (557K) -
藤本 治宏, 山本 一美, 有沢 光弘, 高橋 幸子, 田中 由紀子, 山崎 幹夫1998 年 1998 巻 46 号 p. 29-34
発行日: 1998/01/31
公開日: 2009/08/04
ジャーナル フリーA new metabolite named emecorrugatin A, which caused lethal paralysis in mice, and its analogue named emecorrugatin B were isolated from an Ascomycete, Emericella corrugata, together with two known toxic metabolites, sterigmatocystin and norsolorinic acid.抄録全体を表示PDF形式でダウンロード (630K) -
長嶋 等, 石崎 泰樹, 西田 昌道, 森田 育男, 室田 誠逸, 後藤 哲久1998 年 1998 巻 46 号 p. 35-37
発行日: 1998/01/31
公開日: 2009/08/04
ジャーナル フリーExposure to rubratoxin B caused homozygous null p53 cells to shrink, condense their chromo-somes and fragment their nuclei, showing that rubratoxin B induces apoptosis in p53-null cells抄録全体を表示PDF形式でダウンロード (1513K) -
鶴田 理, 上村 尚, 高橋 治男1998 年 1998 巻 46 号 p. 39-49
発行日: 1998/01/31
公開日: 2009/08/04
ジャーナル フリーマイコトキシン研究会は発足以来ほぼ20年を数える.この間農産物・医薬品素材などを汚染するカビと,それらのカビが産出するマイコトキシンに関して多面的な研究を積み重ね,1988年には我が国でマイコトキシンの国際会議を開催している.会員は医学,薬学,理・化学,農学等の広い専門分野よりなり,国内外で汚染実態の究明や防止対策,マイコトキシンの化学・生体への作用機作などに取り組み,研究成果等を公表して意見交換を積極的に行い,食品・飼料・生薬などの安全供給に貢献してきている. マイコトキシン研究会はマイコトキシン研究への理解をより多くの人に深めること,かつ研究会会員のもつ知識ならびに技術を現場に伝達することを目的に平成6年からセミナーを開催することを企画した.本項は平成6年6月4日第1回セミナーから平成9年3月13～14日第4回セミナーについてセミナー開催世話人から寄せられた資料を収録したものである.抄録全体を表示PDF形式でダウンロード (7213K)
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